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ANGPT4 elisa kit :: Human ANGPT4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504450
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ANGPT4, ELISA Kit

★Popular Item★
 Also Known As   

Human ANGPT4 (Angiopoietin 4) ELISA Kit

 Product Gene Name   

ANGPT4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603705
 3D Structure    ModBase 3D Structure for Q9Y264
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ANGPT4. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ANGPT4 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANGPT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANGPT4 purchase    MBS2504450 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ANGPT4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ANGPT4, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2504450 kit is based on ANGPT4 antibody-ANGPT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANGPT4 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPT4 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPT4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ANGPT4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPT4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ANGPT4. You can calculate the concentration of Human ANGPT4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ANGPT4 elisa kit    ANGPT4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504450. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANGPT4. It may not necessarily be applicable to this product.
 NCBI GI #    7705276
 NCBI GeneID    51378
 NCBI Accession #    NP_057069.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_015985.2 [Other Products]
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 UniProt Primary Accession #    Q9Y264 [Other Products]
 UniProt Secondary Accession #    Q5TFF4; Q9H4Z4; B4E3J9 [Other Products]
 UniProt Related Accession #    Q9Y264 [Other Products]
 Molecular Weight    46,416 Da
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 NCBI Official Full Name    angiopoietin-4
 NCBI Official Synonym Full Names    angiopoietin 4
 NCBI Official Symbol    ANGPT4 [Similar Products]
 NCBI Official Synonym Symbols   
AGP4; ANG4; ANG-3
[Similar Products]
 NCBI Protein Information    angiopoietin-4; ANG-4; angiopoietin-3; dJ824F16.2 (angiopoietin 4)
 UniProt Protein Name    Angiopoietin-4
 UniProt Synonym Protein Names   
Angiopoietin-3; ANG-3
 Protein Family    Angiopoietin
 UniProt Gene Name    ANGPT4 [Similar Products]
 UniProt Synonym Gene Names    ANG3; ANG4; ANG-4; ANG-3 [Similar Products]
 UniProt Entry Name    ANGP4_HUMAN
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 NCBI Summary for ANGPT4    Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ANGPT4    ANGPT4: Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; receptor tyrosine kinase binding

Biological Process: negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization); endothelial cell proliferation; positive regulation of blood vessel endothelial cell migration; angiogenesis; negative regulation of blood vessel endothelial cell migration; blood coagulation; leukocyte migration; negative regulation of apoptosis
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 Research Articles on ANGPT4    1. acute-phase protein A1AT is a physiological regulator of angptl4, another acute-phase protein.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANGPT4 elisa kitPathways associated with ANGPT4 elisa kit
 Reference Product  PubMed Publications
 ANGPT2 elisa kit  >5 publications with ANGPT4 and ANGPT2
 LPL elisa kit  >1 publications with ANGPT4 and LPL
 TIE1 elisa kit  >1 publications with ANGPT4 and TIE1
 TEK elisa kit  >1 publications with ANGPT4 and TEK
 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram
 Tie2 Signaling Pathway antibodies  Tie2 Signaling Pathway Diagram
Diseases associated with ANGPT4 elisa kitOrgans/Tissues associated with ANGPT4 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with ANGPT4 and Disease Models, Animal
 Nervous System Diseases Antibodies  >1 publications with ANGPT4 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >1 publications with ANGPT4 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1 publications with ANGPT4 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >2 publications with ANGPT4 and Uterus
 Placenta Antibodies  >2 publications with ANGPT4 and Placenta
 Testis Antibodies  >1 publications with ANGPT4 and Testis
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