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ATG12 elisa kit :: Human ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS944212
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $510 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,565 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,800 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ATG12 autophagy related 12 homolog (S. cerevisiae), ELISA Kit

 Also Known As   

Human Ubiquitin-like protein ATG12, ATG12 ELISA Kit

 Product Synonym Names    Human Ubiquitin-like protein ATG12 (ATG12) ELISA kit; APG12; APG12L; FBR93; HAPG12; APG12 autophagy 12-like; Apg12 (autophagy; yeast) homolog; ATG12 autophagy related 12 homolog (S. cerevisiae)
 Product Gene Name   

ATG12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS944212 Testing Data    Testing Data PDF
 OMIM    609608
 3D Structure    ModBase 3D Structure for O94817
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ATG12. No significant cross-reactivity or interference between human ATG12 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human ATG12 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of ATG12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ATG12 purchase    MBS944212 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing ATG12, with an estimated sensitivity of The minimum detectable dose of human ATG12 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS944212 kit is based on ATG12 antibody-ATG12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATG12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATG12. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ATG12 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ATG12 elisa kit    ATG12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS944212
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NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.
 NCBI GI #    480540330
 NCBI GeneID    9140
 NCBI Accession #    NP_001264712.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001277783.1 [Other Products]
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 UniProt Primary Accession #    O94817 [Other Products]
 UniProt Secondary Accession #    Q6PJV2 [Other Products]
 UniProt Related Accession #    O94817 [Other Products]
 Molecular Weight    15,113 Da
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 NCBI Official Full Name    ubiquitin-like protein ATG12 isoform 2
 NCBI Official Synonym Full Names    autophagy related 12
 NCBI Official Symbol    ATG12 [Similar Products]
 NCBI Official Synonym Symbols   
APG12; FBR93; APG12L; HAPG12
[Similar Products]
 NCBI Protein Information    ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog
 UniProt Protein Name    Ubiquitin-like protein ATG12
 UniProt Synonym Protein Names   
Autophagy-related protein 12; APG12-like
 UniProt Gene Name    ATG12 [Similar Products]
 UniProt Synonym Gene Names    APG12; APG12L; APG12-like [Similar Products]
 UniProt Entry Name    ATG12_HUMAN
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 NCBI Summary for ATG12    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for ATG12    ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; autophagic vacuole formation; cellular response to nitrogen starvation
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 Research Articles on ATG12    1. Cav-1 competitively interacts with the ATG12-ATG5 system to suppress the formation and function of the latter in lung epithelial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ATG12 elisa kitPathways associated with ATG12 elisa kit
 Reference Product  PubMed Publications
 ATG5 elisa kit  >108 publications with ATG12 and ATG5
 ATG7 elisa kit  >40 publications with ATG12 and ATG7
 ATG3 elisa kit  >19 publications with ATG12 and ATG3
 ATG10 elisa kit  >19 publications with ATG12 and ATG10
 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
Diseases associated with ATG12 elisa kitOrgans/Tissues associated with ATG12 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with ATG12 and Nervous System Diseases
 Neoplasms Antibodies  >19 publications with ATG12 and Neoplasms
 Necrosis Antibodies  >7 publications with ATG12 and Necrosis
 Drug Toxicity Antibodies  >5 publications with ATG12 and Drug Toxicity
 Cardiovascular Diseases Antibodies  >4 publications with ATG12 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with ATG12 and Heart Diseases
 Muscular Diseases Antibodies  >2 publications with ATG12 and Muscular Diseases
 Lung Neoplasms Antibodies  >2 publications with ATG12 and Lung Neoplasms
 Cardiomyopathies Antibodies  >2 publications with ATG12 and Cardiomyopathies
 Ataxia Antibodies  >2 publications with ATG12 and Ataxia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with ATG12 and Brain
 Muscle Antibodies  >12 publications with ATG12 and Muscle
 Liver Antibodies  >7 publications with ATG12 and Liver
 Nerve Antibodies  >7 publications with ATG12 and Nerve
 Blood Antibodies  >5 publications with ATG12 and Blood
 Heart Antibodies  >4 publications with ATG12 and Heart
 Stomach Antibodies  >4 publications with ATG12 and Stomach
 Lung Antibodies  >4 publications with ATG12 and Lung
 Prostate Antibodies  >2 publications with ATG12 and Prostate
 Vascular Antibodies  >2 publications with ATG12 and Vascular
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