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ABCB11 elisa kit :: Human ATP-binding cassette, sub-family B (MDR/TAP) , member 11 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS946530
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ATP-binding cassette, sub-family B (MDR/TAP) , member 11 (ABCB11), ELISA Kit

 Also Known As   

Human Bile salt export pump, ABCB11 ELISA Kit

 Product Synonym Names    Human Bile salt export pump (ABCB11) ELISA kit; ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP; ABC member 16; MDR/TAP subfamily; bile salt export pump; progressive familial intrahepatic cholestasis 2; sister p-glycoprotein; ATP-binding cassette; sub-family B (MDR/TAP); member 11
 Product Gene Name   

ABCB11 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS946530 Testing Data    Testing Data PDF
 OMIM    gene 605479
 3D Structure    ModBase 3D Structure for O95342
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ABCB11. No significant cross-reactivity or interference between human ABCB11 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of human ABCB11 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ABCB11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ABCB11 purchase    MBS946530 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP-binding cassette, sub-family B (MDR/TAP), member 11 (ABCB11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing ABCB11, with an estimated sensitivity of The minimum detectable dose of human ABCB11 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS946530 kit is based on ABCB11 antibody-ABCB11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABCB11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABCB11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ABCB11 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABCB11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCB11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB11 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ABCB11 elisa kit    ABCB11 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS946530. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ABCB11. It may not necessarily be applicable to this product.
 NCBI GI #    21536378
 NCBI GeneID    8647
 NCBI Accession #    NP_003733.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003742.2 [Other Products]
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 UniProt Primary Accession #    O95342 [Other Products]
 UniProt Secondary Accession #    Q53TL2; Q9UNB2 [Other Products]
 UniProt Related Accession #    O95342 [Other Products]
 Molecular Weight    146,407 Da
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 NCBI Official Full Name    bile salt export pump
 NCBI Official Synonym Full Names    ATP-binding cassette, sub-family B (MDR/TAP), member 11
 NCBI Official Symbol    ABCB11 [Similar Products]
 NCBI Official Synonym Symbols   
BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
[Similar Products]
 NCBI Protein Information    bile salt export pump; sister p-glycoprotein; ABC member 16, MDR/TAP subfamily; ATP-binding cassette sub-family B member 11; progressive familial intrahepatic cholestasis 2
 UniProt Protein Name    Bile salt export pump
 UniProt Synonym Protein Names   
ATP-binding cassette sub-family B member 11
 Protein Family    ABC transporter B family
 UniProt Gene Name    ABCB11 [Similar Products]
 UniProt Synonym Gene Names    BSEP [Similar Products]
 UniProt Entry Name    ABCBB_HUMAN
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 NCBI Summary for ABCB11    The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ABCB11    ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Transporter, ABC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: intercellular canaliculus; membrane; apical part of cell; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: transporter activity; ATPase activity, coupled to transmembrane movement of substances; canalicular bile acid transmembrane transporter activity; bile acid-exporting ATPase activity; sodium-exporting ATPase activity, phosphorylative mechanism; ATP binding

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; transport; bile acid metabolic process; canalicular bile acid transport; transmembrane transport

Disease: Cholestasis, Progressive Familial Intrahepatic, 2; Cholestasis, Benign Recurrent Intrahepatic, 2
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 Research Articles on ABCB11    1. Patients carrying the C allele in the ABCB11 1331T>C polymorphism are at increased risk of developing hepatocellular type of DILI, when taking drugs containing a carbocyclic system with aromatic rings
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ABCB11 elisa kitPathways associated with ABCB11 elisa kit
 Reference Product  PubMed Publications
 HAX1 elisa kit  >1 publications with ABCB11 and HAX1
 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 Recycling Of Bile Acids And Salts Pathway antibodies  Recycling Of Bile Acids And Salts Pathway Diagram
 Synthesis Of Bile Acids And Bile Salts Pathway antibodies  Synthesis Of Bile Acids And Bile Salts Pathway Diagram
Diseases associated with ABCB11 elisa kitOrgans/Tissues associated with ABCB11 elisa kit
 Disease Name  Pubmed Publications
 Cholestasis Antibodies  >198 publications with ABCB11 and Cholestasis
 Liver Diseases Antibodies  >154 publications with ABCB11 and Liver Diseases
 Cholestasis, Intrahepatic Antibodies  >139 publications with ABCB11 and Cholestasis, Intrahepatic
 Liver Cirrhosis Antibodies  >23 publications with ABCB11 and Liver Cirrhosis
 Gallstones Antibodies  >20 publications with ABCB11 and Gallstones
 Cholelithiasis Antibodies  >18 publications with ABCB11 and Cholelithiasis
 Inflammation Antibodies  >18 publications with ABCB11 and Inflammation
 Pruritus Antibodies  >18 publications with ABCB11 and Pruritus
 Neoplasms Antibodies  >17 publications with ABCB11 and Neoplasms
 Liver Failure Antibodies  >17 publications with ABCB11 and Liver Failure
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >277 publications with ABCB11 and Liver
 Lung Antibodies  >2 publications with ABCB11 and Lung
 Testis Antibodies  >1 publications with ABCB11 and Testis
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