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ACAT1 elisa kit :: Human Acetyl-CoA acetyltransferase, mitochondrial ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762332
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1), ELISA Kit

 Also Known As   

Human Acetyl-CoA acetyltransferase, mitochondrial ELISA Kit

 Product Synonym Names    ACAT1/AACAT/MAT/Acetyl-CoA acetyltransferase; mitochondrial/Acetoacetyl-CoA thiolase/T2
 Product Gene Name   

ACAT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    203750
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 Species Reactivity    Human
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    7.8-500pg/ml
 Sensitivity    4.688pg/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACAT1 purchase    MBS762332 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8-500pg/ml in biological research samples containing ACAT1, with an estimated sensitivity of 4.688pg/ml. The ELISA analytical biochemical technique of the MBS762332 kit is based on ACAT1 antibody-ACAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACAT1 elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ACAT1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ACAT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ACAT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman ACAT1can be calculated.
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 Typical Testing Data of ACAT1 elisa kit    ACAT1 elisa kit Typical Testing Data image
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 Typical Standard Curve of ACAT1 elisa kit    ACAT1 elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS762332. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.
 NCBI GI #    4557237
 NCBI GeneID    38
 NCBI Accession #    NP_000010.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000019.3 [Other Products]
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 UniProt Secondary Accession #    Q96FG8; B2R6H1; G3XAB4 [Other Products]
 UniProt Related Accession #    P24752 [Other Products]
 Molecular Weight    17,175 Da
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 NCBI Official Full Name    acetyl-CoA acetyltransferase, mitochondrial
 NCBI Official Synonym Full Names    acetyl-CoA acetyltransferase 1
 NCBI Official Symbol    ACAT1 [Similar Products]
 NCBI Official Synonym Symbols   
T2; MAT; ACAT; THIL
[Similar Products]
 NCBI Protein Information    acetyl-CoA acetyltransferase, mitochondrial
 UniProt Protein Name    Acetyl-CoA acetyltransferase, mitochondrial
 UniProt Synonym Protein Names   
Acetoacetyl-CoA thiolase; T2
 UniProt Gene Name    ACAT1 [Similar Products]
 UniProt Synonym Gene Names    ACAT; MAT [Similar Products]
 UniProt Entry Name    THIL_HUMAN
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 NCBI Summary for ACAT1    This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
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 UniProt Comments for ACAT1    ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Lipid Metabolism - fatty acid; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Mitochondrial; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - lysine degradation; EC 2.3.1.9; Amino Acid Metabolism - tryptophan; Acetyltransferase; Lipid Metabolism - synthesis and degradation of ketone bodies

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: acetyl-CoA C-acetyltransferase activity; coenzyme binding; enzyme binding; metal ion binding; protein homodimerization activity

Biological Process: brain development; branched chain family amino acid catabolic process; cellular lipid metabolic process; ketone body biosynthetic process; ketone body catabolic process; ketone body metabolic process; liver development; protein homooligomerization; response to hormone stimulus; response to organic cyclic substance; response to starvation

Disease: Alpha-methylacetoacetic Aciduria
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 Research Articles on ACAT1    1. Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACAT1 elisa kitPathways associated with ACAT1 elisa kit
 Reference Product  PubMed Publications
 HMGCS2 elisa kit  >6 publications with ACAT1 and HMGCS2
 OXCT1 elisa kit  >5 publications with ACAT1 and OXCT1
 EHHADH elisa kit  >2 publications with ACAT1 and EHHADH
 ACACB elisa kit  >1 publications with ACAT1 and ACACB
 ACACA elisa kit  >1 publications with ACAT1 and ACACA
 Products by Pathway  Pathway Diagram
 Branched-chain Amino Acid Catabolism Pathway antibodies  Branched-chain Amino Acid Catabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
Diseases associated with ACAT1 elisa kitOrgans/Tissues associated with ACAT1 elisa kit
 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >81 publications with ACAT1 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >57 publications with ACAT1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >21 publications with ACAT1 and Nervous System Diseases
 Brain Diseases Antibodies  >18 publications with ACAT1 and Brain Diseases
 Dyslipidemias Antibodies  >15 publications with ACAT1 and Dyslipidemias
 Fatty Liver Antibodies  >14 publications with ACAT1 and Fatty Liver
 Hyperlipidemias Antibodies  >14 publications with ACAT1 and Hyperlipidemias
 Liver Diseases Antibodies  >13 publications with ACAT1 and Liver Diseases
 Inflammation Antibodies  >11 publications with ACAT1 and Inflammation
 Necrosis Antibodies  >10 publications with ACAT1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >51 publications with ACAT1 and Blood
 Liver Antibodies  >50 publications with ACAT1 and Liver
 Intestine Antibodies  >22 publications with ACAT1 and Intestine
 Brain Antibodies  >16 publications with ACAT1 and Brain
 Muscle Antibodies  >15 publications with ACAT1 and Muscle
 Bone Antibodies  >12 publications with ACAT1 and Bone
 Heart Antibodies  >8 publications with ACAT1 and Heart
 Ovary Antibodies  >7 publications with ACAT1 and Ovary
 Adipose Tissue Antibodies  >7 publications with ACAT1 and Adipose Tissue
 Kidney Antibodies  >6 publications with ACAT1 and Kidney
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