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AChR elisa kit :: Human Acetylcholine Receptor Antibody (AChR) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729942
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acetylcholine Receptor Antibody (AChR), ELISA Kit

 Also Known As   

Human Anti-Acetylcholine Receptor Antibody (Anti-AChR) ELISA Kit

 Product Synonym Names    Human Anti-Acetylcholine Receptor Antibody (Anti-AChR) ELISA Kit; Anti-Acetylcholine Receptor Antibody (Anti-AChR); Anti-Acetylcholine Receptor Antibody ELISA Kit (Anti-AChR) (Human)
 Product Gene Name   

AChR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    PDF Manual
 MBS729942 COA    COA PDF
 MBS729942 MSDS    MSDS PDF
 Chromosome Location    Chromosome: 2; NC_000002.11 (233390870..233401375). Location: 2q37.1
 OMIM    100720
 3D Structure    ModBase 3D Structure for Q07001
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AchRAb. No significant cross-reactivity or interference between AchRAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AchRAb and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of AChR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AChR purchase    MBS729942 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholine Receptor Antibody (AChR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing AChR, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS729942 kit is based on AChR antibody-AChR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AChR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AChR. Appropriate sample types include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AChR elisa kit

   Intended Uses||This AchRAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AchRAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: AchRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti Acetylcholine Receptor antigen and an AchRAb-HRP conjugate. The assay sample and buffer are incubated together with AchRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AchRAb concentration since AchRAb from samples and AchRAb-HRP conjugate compete for the Anti Acetylcholine Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by AchRAb from the sample, fewer sites are left to bind AchRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AchRAb concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AChR elisa kit    Human ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of AChR elisa kit    AChR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for AChR. It may not necessarily be applicable to this product.
 NCBI GI #    297402
 NCBI GeneID    1144
 NCBI Accession #    CAA38759.1 [Other Products]
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 UniProt Primary Accession #    Q07001 [Other Products]
 UniProt Secondary Accession #    Q52LH4; A8K661; B4DT92 [Other Products]
 UniProt Related Accession #    Q07001 [Other Products]
 Molecular Weight    58,895 Da
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 NCBI Official Full Name    acetylcholine receptor delta subunit
 NCBI Official Synonym Full Names    cholinergic receptor, nicotinic, delta (muscle)
 NCBI Official Symbol    CHRND [Similar Products]
 NCBI Official Synonym Symbols   

ACHRD; CMS2A; FCCMS; SCCMS

[Similar Products]
 NCBI Protein Information    acetylcholine receptor subunit delta; acetylcholine receptor, nicotinic, delta (muscle); cholinergic receptor, nicotinic, delta polypeptide
 UniProt Protein Name    Acetylcholine receptor subunit delta
 UniProt Gene Name    CHRND [Similar Products]
 UniProt Synonym Gene Names    ACHRD [Similar Products]
 UniProt Entry Name    ACHD_HUMAN
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 NCBI Summary for AChR    The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL), congenital myasthenic syndrome slow-channel type (SCCMS), and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Feb 2012]
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 UniProt Comments for AChR    nAChRD: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRND are a cause of multiple pterygium syndrome lethal type (MUPSL). Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent. Defects in CHRND are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRND are a cause of congenital myasthenic syndrome fast-channel type (FCCMS). FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Delta/CHRND sub-subfamily.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; cell junction

Molecular Function: nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: skeletal muscle contraction; synaptic transmission; regulation of membrane potential; muscle contraction; transport; neuromuscular process; skeletal muscle growth; signal transduction; musculoskeletal movement

Disease: Myasthenic Syndrome, Congenital, 3a, Slow-channel; Myasthenic Syndrome, Congenital, 3c, Associated With Acetylcholine Receptor Deficiency; Multiple Pterygium Syndrome, Lethal Type; Myasthenic Syndrome, Congenital, 3b, Fast-channel
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 Research Articles on AChR    1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AChR elisa kitPathways associated with AChR elisa kit
 Reference Product  PubMed Publications
 CHRNA1 elisa kit  >10 publications with AChR and CHRNA1
 CHRNB1 elisa kit  >7 publications with AChR and CHRNB1
 CHRNE elisa kit  >6 publications with AChR and CHRNE
 CHRNA3 elisa kit  >4 publications with AChR and CHRNA3
 CHRNA5 elisa kit  >4 publications with AChR and CHRNA5
 CHRNB3 elisa kit  >4 publications with AChR and CHRNB3
 CHRNA4 elisa kit  >3 publications with AChR and CHRNA4
 CHRNA6 elisa kit  >3 publications with AChR and CHRNA6
 CHRNA2 elisa kit  >1 publications with AChR and CHRNA2
 ALPI elisa kit  >1 publications with AChR and ALPI
 Products by Pathway  Pathway Diagram
 Acetylcholine Binding And Downstream Events Pathway antibodies  Acetylcholine Binding And Downstream Events Pathway Diagram
 Activation Of Nicotinic Acetylcholine Receptors Pathway antibodies  Activation Of Nicotinic Acetylcholine Receptors Pathway Diagram
 Highly Sodium Permeable Acetylcholine Nicotinic Receptors Pathway antibodies  Highly Sodium Permeable Acetylcholine Nicotinic Receptors Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Postsynaptic Nicotinic Acetylcholine Receptors Pathway antibodies  Postsynaptic Nicotinic Acetylcholine Receptors Pathway Diagram
 Presynaptic Nicotinic Acetylcholine Receptors Pathway antibodies  Presynaptic Nicotinic Acetylcholine Receptors Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram
Diseases associated with AChR elisa kitOrgans/Tissues associated with AChR elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >10 publications with AChR and Nervous System Diseases
 MULTIPLE PTERYGIUM SYNDROME, LETHAL TYPE Antibodies  >4 publications with AChR and MULTIPLE PTERYGIUM SYNDROME, LETHAL TYPE
 Musculoskeletal Abnormalities Antibodies  >3 publications with AChR and Musculoskeletal Abnormalities
 Brain Diseases Antibodies  >1 publications with AChR and Brain Diseases
 Myasthenic Syndrome, Congenital, Fast-Channel Antibodies  >1 publications with AChR and Myasthenic Syndrome, Congenital, Fast-Channel
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with AChR and Muscle
 Lung Antibodies  >1 publications with AChR and Lung
 Heart Antibodies  >1 publications with AChR and Heart
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