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AchE elisa kit :: Human Acetylcholinesterase (AchE) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024347 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acetylcholinesterase (AchE), ELISA Kit

★Popular Item★
 Also Known As   

Acetylcholinesterase (AchE) ELISA Kit

 Product Synonym Names    ARAChE; N-AChE; YT
 Product Gene Name   

AchE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    100740
 3D Structure    ModBase 3D Structure for P22303
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of acetylcholinesterase. No significant cross-reactivity or interference between acetylcholinesterase and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    3.12-200ng/mL
 Sensitivity    <1.34ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level acetylcholinesterase were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level acetylcholinesterase were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AchE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AchE purchase    MBS2024347 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholinesterase (AchE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12-200ng/mL in biological research samples containing AchE, with an estimated sensitivity of <1.34ng/mL. The ELISA analytical biochemical technique of the MBS2024347 kit is based on AchE antibody-AchE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AchE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AchE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AchE elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of acetylcholinesterase in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to acetylcholinesterase. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to acetylcholinesterase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain acetylcholinesterase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of acetylcholinesterase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for AchE elisa kit    Signal transduction; Enzyme & Kinase; Metabolic pathway; Hematology; Neuroscience
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  Typical Testing Data/Standard Curve (for reference only) of AchE elisa kit    AchE elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024347. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AchE. It may not necessarily be applicable to this product.
 NCBI GI #    545225
 NCBI GeneID    43
 NCBI Accession #    AAC60618.1 [Other Products]
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 UniProt Primary Accession #    P22303 [Other Products]
 UniProt Secondary Accession #    Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7 [Other Products]
 UniProt Related Accession #    P22303 [Other Products]
 Molecular Weight    58,352 Da
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 NCBI Official Full Name    acetylcholinesterase, partial
 NCBI Official Synonym Full Names    acetylcholinesterase (Yt blood group)
 NCBI Official Symbol    ACHE [Similar Products]
 NCBI Official Synonym Symbols   
YT; ACEE; ARACHE; N-ACHE
[Similar Products]
 NCBI Protein Information    acetylcholinesterase
 UniProt Protein Name    Acetylcholinesterase
 Protein Family    Acetylcholinesterase
 UniProt Gene Name    ACHE [Similar Products]
 UniProt Synonym Gene Names    AChE [Similar Products]
 UniProt Entry Name    ACES_HUMAN
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 NCBI Summary for AchE    Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AchE    ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Hydrolase; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; basal lamina; synapse; neuromuscular junction; cell junction; nucleus

Molecular Function: collagen binding; protein binding; serine hydrolase activity; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: nervous system development; negative regulation of synaptic transmission, cholinergic; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; synaptic transmission; cell proliferation; synaptogenesis; positive regulation of protein secretion; amyloid precursor protein metabolic process; retina development in camera-type eye; neurotransmitter receptor biosynthetic process; receptor internalization; phospholipid metabolic process; phosphatidylcholine biosynthetic process; response to wounding; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen
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 Research Articles on AchE    1. Data suggest that natural antisense RNA may play an important role in acetylcholinesterase (AChE) regulation via affecting the epigenetic modification in the AChE promoter region.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AchE elisa kitDiseases associated with AchE elisa kit
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >235805 publications with AchE and Nervous System Diseases
 Brain Diseases Antibodies  >30313 publications with AchE and Brain Diseases
 Inflammation Antibodies  >23801 publications with AchE and Inflammation
 Necrosis Antibodies  >12683 publications with AchE and Necrosis
 Hypertension Antibodies  >11649 publications with AchE and Hypertension
 Kidney Diseases Antibodies  >10945 publications with AchE and Kidney Diseases
 Edema Antibodies  >10839 publications with AchE and Edema
 Liver Diseases Antibodies  >8919 publications with AchE and Liver Diseases
 Weight Loss Antibodies  >7099 publications with AchE and Weight Loss
 Neurobehavioral Manifestations Antibodies  >6429 publications with AchE and Neurobehavioral Manifestations
Organs/Tissues associated with AchE elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >79273 publications with AchE and Blood
 Nerve Antibodies  >46806 publications with AchE and Nerve
 Muscle Antibodies  >42294 publications with AchE and Muscle
 Heart Antibodies  >41298 publications with AchE and Heart
 Brain Antibodies  >36724 publications with AchE and Brain
 Liver Antibodies  >13975 publications with AchE and Liver
 Kidney Antibodies  >13168 publications with AchE and Kidney
 Intestine Antibodies  >11981 publications with AchE and Intestine
 Lung Antibodies  >11863 publications with AchE and Lung
 Mouth Antibodies  >10177 publications with AchE and Mouth
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