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APC elisa kit :: Human Activated protein C ELISA Kit

Scan QR to view Datasheet Catalog #    MBS730738
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Activated protein C (APC), ELISA Kit

 Also Known As   

Human Activated protein C ELISA Kit

 Product Gene Name   

APC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613659
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of APC. No significant cross-reactivity or interference between APC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APC and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    100-2500pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This APC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human APC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APC purchase    MBS730738 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activated protein C (APC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100-2500pg/mL in biological research samples containing APC, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS730738 kit is based on APC antibody-APC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APC elisa kit

   Principle of the assay: APC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APC. Standards or samples are then added to the microtiter plate wells and APC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for APC are added to each well to “sandwich” the APC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APC concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for APC elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of APC elisa kit    APC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS730738. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APC. It may not necessarily be applicable to this product.
 NCBI GI #    53759122
 NCBI GeneID    324
 NCBI Accession #    NP_000029.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000038.5 [Other Products]
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 UniProt Secondary Accession #    Q15162; Q15163; Q93042; D3DT03 [Other Products]
 UniProt Related Accession #    P25054 [Other Products]
 Molecular Weight    300,439 Da
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 NCBI Official Full Name    adenomatous polyposis coli protein isoform b
 NCBI Official Synonym Full Names    adenomatous polyposis coli
 NCBI Official Symbol    APC [Similar Products]
 NCBI Official Synonym Symbols   
GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
[Similar Products]
 NCBI Protein Information    adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
 UniProt Protein Name    Adenomatous polyposis coli protein
 UniProt Synonym Protein Names   
Deleted in polyposis 2.5
 Protein Family    Adenomatous polyposis coli
 UniProt Gene Name    APC [Similar Products]
 UniProt Synonym Gene Names    DP2.5; Protein APC [Similar Products]
 UniProt Entry Name    APC_HUMAN
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 NCBI Summary for APC    This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APC    APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; beta-catenin destruction complex; cytosol; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; somatic stem cell maintenance; positive regulation of apoptosis; apoptosis; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; kidney development; cell cycle arrest; cell adhesion; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; dorsal/ventral pattern formation; axonogenesis; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma
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 Research Articles on APC    1. Colorectal tumors from APC*I1307K carriers principally harbor somatic APC mutations outside the A8 tract
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APC elisa kitPathways associated with APC elisa kit
 Reference Product  PubMed Publications
 CTNNB1 elisa kit  >1774 publications with APC and CTNNB1
 AXIN1 elisa kit  >83 publications with APC and AXIN1
 AXIN2 elisa kit  >79 publications with APC and AXIN2
 MAPRE1 elisa kit  >13 publications with APC and MAPRE1
 BTRC elisa kit  >9 publications with APC and BTRC
 ARHGEF4 elisa kit  >9 publications with APC and ARHGEF4
 CTBP1 elisa kit  >4 publications with APC and CTBP1
 GSK3B elisa kit  >4 publications with APC and GSK3B
 CSNK1E elisa kit  >1 publications with APC and CSNK1E
 Products by Pathway  Pathway Diagram
 AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies  AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram
 APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway antibodies  APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway Diagram
 APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies  APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram
 AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies  AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram
 AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway antibodies  AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
Diseases associated with APC elisa kitOrgans/Tissues associated with APC elisa kit
 Disease Name  Pubmed Publications
 Gastrointestinal Neoplasms Antibodies  >3843 publications with APC and Gastrointestinal Neoplasms
 Intestinal Neoplasms Antibodies  >3590 publications with APC and Intestinal Neoplasms
 Colorectal Neoplasms Antibodies  >3382 publications with APC and Colorectal Neoplasms
 Adenomatous Polyposis Coli Antibodies  >3347 publications with APC and Adenomatous Polyposis Coli
 Colonic Neoplasms Antibodies  >2282 publications with APC and Colonic Neoplasms
 Adenoma Antibodies  >2191 publications with APC and Adenoma
 Adenomatous Polyps Antibodies  >1734 publications with APC and Adenomatous Polyps
 Inflammation Antibodies  >994 publications with APC and Inflammation
 Adenocarcinoma Antibodies  >894 publications with APC and Adenocarcinoma
 Necrosis Antibodies  >562 publications with APC and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7058 publications with APC and Blood
 Intestine Antibodies  >1084 publications with APC and Intestine
 Bone Antibodies  >808 publications with APC and Bone
 Spleen Antibodies  >765 publications with APC and Spleen
 Vascular Antibodies  >724 publications with APC and Vascular
 Lymph Node Antibodies  >700 publications with APC and Lymph Node
 Liver Antibodies  >693 publications with APC and Liver
 Lung Antibodies  >639 publications with APC and Lung
 Brain Antibodies  >620 publications with APC and Brain
 Skin Antibodies  >514 publications with APC and Skin
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