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ASCC3 elisa kit :: Human Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024856 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), ELISA Kit

 Also Known As   

Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit

 Product Synonym Names    HELIC1; RNAH; ASC1p200; RNA Helicase Family; Helicase, ATP Binding 1; Trip4 complex subunit p200; ASC-1 complex subunit p200
 Product Gene Name   

ASCC3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3).
No significant cross-reactivity or interference between Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.122ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ASCC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ASCC3 purchase    MBS2024856 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing ASCC3, with an estimated sensitivity of < 0.122ng/mL. The ELISA analytical biochemical technique of the MBS2024856 kit is based on ASCC3 antibody-ASCC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASCC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASCC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ASCC3 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ASCC3 elisa kit    Tumor immunity
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 Typical Testing Data/Standard Curve (for reference only) of ASCC3 elisa kit    ASCC3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024856. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ASCC3. It may not necessarily be applicable to this product.
 NCBI GI #    225703058
 NCBI GeneID    77987
 NCBI Accession #    NP_932124.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_198007.2 [Other Products]
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 UniProt Secondary Accession #    Q6PB36; Q8C1G1; Q8C707; Q8K292 [Other Products]
 UniProt Related Accession #    E9PZJ8 [Other Products]
 Molecular Weight    128,982 Da
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 NCBI Official Full Name    activating signal cointegrator 1 complex subunit 3
 NCBI Official Synonym Full Names    activating signal cointegrator 1 complex subunit 3
 NCBI Official Symbol    Ascc3 [Similar Products]
 NCBI Official Synonym Symbols   
RNAH; Helic1; ASC1p200; BC023451; D630041L21; B630009I04Rik; D430001L07Rik
[Similar Products]
 NCBI Protein Information    activating signal cointegrator 1 complex subunit 3
 UniProt Protein Name    Activating signal cointegrator 1 complex subunit 3
 Protein Family    Activating signal cointegrator 1 complex
 UniProt Gene Name    Ascc3 [Similar Products]
 UniProt Entry Name    ASCC3_MOUSE
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 UniProt Comments for ASCC3    ASCC3: 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3, enabling ALKHB3 to process alkylated N3- methylcytosine (3mC) within double-stranded regions. Enhances NF- kappa-B, SRF and AP1 transactivation. Belongs to the helicase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; Transcription, coactivator/corepressor

Cellular Component: Golgi apparatus; membrane; cytoplasm; intracellular; nucleus

Molecular Function: ATP-dependent 3'-5' DNA helicase activity; nucleic acid binding; hydrolase activity; nucleotide binding; helicase activity; ATP binding

Biological Process: cell proliferation; DNA dealkylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA repair; response to DNA damage stimulus; DNA duplex unwinding
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ASCC3 elisa kitDiseases associated with ASCC3 elisa kit
 Reference Product  PubMed Publications
 Cdc5l elisa kit  >1 publications with ASCC3 and Cdc5l
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >1 publications with ASCC3 and Prostatic Neoplasms
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