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INHBA elisa kit :: Human Activin A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175781
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $380 +1 FREE 8GB USB
 
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 Product Name   

Activin A (INHBA), ELISA Kit

★Popular Item★
 Also Known As   

Human Activin A PicoKine ELISA Kit

 Product Synonym Names    Inhibin beta A chain; inhibin, beta A; Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain
 Product Gene Name   

INHBA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for A4D1W7
 Species Reactivity    Human
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 Specificity    Natural and recombinant human Activin A
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 Samples    Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
 Assay Type    Quantitative Sandwich
 Detection Range    15.6pg/ml- 1000pg/ml
 Sensitivity    <12pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of INHBA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for INHBA purchase    MBS175781 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activin A (INHBA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6pg/ml- 1000pg/ml in biological research samples containing INHBA, with an estimated sensitivity of <12pg/ml. The ELISA analytical biochemical technique of the MBS175781 kit is based on INHBA antibody-INHBA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHBA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHBA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, plasma (heparin, EDTA) and saliva. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for INHBA elisa kit

   Principle of the Assay: The Human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(Expression system for standard: CHO ; Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. Acvidin-Biotin-Peroxidase Compiex was added and unbound conugates were washed away with PBS or TBS buffer HRP substrate TMB was used to visualize HRP enzymatic tion. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Activin A amount of sample captured in plate.

Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of Ire transforming growth factor-beta superfamily, is expressed loca!ly by the mesenchymal component of the hemopoietlc microenvironment. its expression is regulated on the mRNA level by different cytoKines. and the biologilt activity of the protein is... controlled by several inhibitory molecules.' Inhibins and activins are members of ine transforming growth factor beta superfamily and are known to modulate the growth and differentiation of severe I cell types 2 Inhibins and activins inhibit and activate. respectively. the secretion of follitropin by the pituitary glanj. Inhibins/activins are Involved in regulating a number of diverse functions such as hypothalamic and pituitary horlnone secretion, gonadal hormone secretion, germ celi development and maturation. erythroid differentiation. insulin secretion. nerve cell survival embryonic axial development or bone growth depending on their subunit composition. Inhibins appear to oppose the functions of activins.
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 Typical Testing Data/Standard Curve (for reference only) of INHBA elisa kit    Human Activin A PicoKine ELISA Kit standard curve
INHBA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175781. Click to request current manual
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NCBI/Uniprot data below describe general gene information for INHBA. It may not necessarily be applicable to this product.
 NCBI GeneID    3624
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 UniProt Primary Accession #    A4D1W7 [Other Products]
 UniProt Related Accession #    P08476 [Other Products]
 Molecular Weight    47,442 Da
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 NCBI Official Synonym Full Names    inhibin, beta A
 NCBI Official Symbol    INHBA [Similar Products]
 NCBI Official Synonym Symbols   
EDF; FRP
[Similar Products]
 NCBI Protein Information    inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)
 UniProt Protein Name    Inhibin, beta A (Activin A, activin AB alpha polypeptide)
 UniProt Synonym Protein Names   
Inhibin, beta A (Activin A, activin AB alpha polypeptide)
 Protein Family    Inhibin
 UniProt Gene Name    INHBA [Similar Products]
 UniProt Entry Name    A4D1W7_HUMAN
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 NCBI Summary for INHBA    The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]
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 UniProt Comments for INHBA    INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; hair follicle development; cell-cell signaling; ovarian follicle development; erythrocyte differentiation; cell differentiation; cell cycle arrest; negative regulation of B cell differentiation; hemoglobin biosynthetic process; response to drug; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; growth; G1/S transition of mitotic cell cycle
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 Research Articles on INHBA    1. We identified an unreported germline mutation of the INHA gene encoding the alpha-subunit, the partner of the betaA-subunit. This mutation also alters the secreted activin/inhibin ratio, by disrupting both inhibin A and inhibin B biosynthesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with INHBA elisa kitDiseases associated with INHBA elisa kit
 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 Antagonism Of Activin By Follistatin Pathway antibodies  Antagonism Of Activin By Follistatin Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Glycoprotein Hormones Pathway antibodies  Glycoprotein Hormones Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Biosynthesis Pathway antibodies  Peptide Hormone Biosynthesis Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >7 publications with INHBA and Necrosis
 Adenocarcinoma Antibodies  >7 publications with INHBA and Adenocarcinoma
 Kidney Diseases Antibodies  >5 publications with INHBA and Kidney Diseases
 Inflammation Antibodies  >5 publications with INHBA and Inflammation
 Disease Models, Animal Antibodies  >5 publications with INHBA and Disease Models, Animal
 Lung Neoplasms Antibodies  >3 publications with INHBA and Lung Neoplasms
 Ovarian Neoplasms Antibodies  >3 publications with INHBA and Ovarian Neoplasms
 Pre-Eclampsia Antibodies  >3 publications with INHBA and Pre-Eclampsia
 Cardiovascular Diseases Antibodies  >2 publications with INHBA and Cardiovascular Diseases
 Hypertrophy Antibodies  >2 publications with INHBA and Hypertrophy
Organs/Tissues associated with INHBA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >23 publications with INHBA and Ovary
 Blood Antibodies  >16 publications with INHBA and Blood
 Bone Antibodies  >15 publications with INHBA and Bone
 Kidney Antibodies  >7 publications with INHBA and Kidney
 Skin Antibodies  >6 publications with INHBA and Skin
 Vascular Antibodies  >6 publications with INHBA and Vascular
 Stomach Antibodies  >6 publications with INHBA and Stomach
 Lung Antibodies  >6 publications with INHBA and Lung
 Placenta Antibodies  >6 publications with INHBA and Placenta
 Brain Antibodies  >5 publications with INHBA and Brain
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