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ADORA2a elisa kit :: Human Adenosine A2a Receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533431
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Adenosine A2a Receptor (ADORA2a), ELISA Kit

★Popular Item★
 Also Known As   

Human ADORA2a (Adenosine A2a Receptor) ELISA Kit

 Product Gene Name   

ADORA2a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2533431 COA    COA PDF
 OMIM    102776
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanADORA2a. No significant cross-reactivity or interference between HumanADORA2a and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADORA2a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADORA2a purchase    MBS2533431 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine A2a Receptor (ADORA2a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing ADORA2a, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2533431 kit is based on ADORA2a antibody-ADORA2a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADORA2a elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanADORA2aconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADORA2a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADORA2aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADORA2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADORA2a.You can calculate the concentration of ADORA2ain the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADORA2a elisa kit    ADORA2a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2533431. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADORA2a. It may not necessarily be applicable to this product.
 NCBI GI #    119580062
 NCBI GeneID    135
 NCBI Accession #    EAW59658.1 [Other Products]
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 UniProt Secondary Accession #    B2R7E0 [Other Products]
 UniProt Related Accession #    P29274 [Other Products]
 Molecular Weight    44,707 Da
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 NCBI Official Full Name    adenosine A2a receptor
 NCBI Official Synonym Full Names    adenosine A2a receptor
 NCBI Official Symbol    ADORA2A [Similar Products]
 NCBI Official Synonym Symbols   
A2aR; RDC8; ADORA2
[Similar Products]
 NCBI Protein Information    adenosine receptor A2a
 UniProt Protein Name    Adenosine receptor A2a
 Protein Family    Adenosine receptor
 UniProt Gene Name    ADORA2A [Similar Products]
 UniProt Synonym Gene Names    ADORA2 [Similar Products]
 UniProt Entry Name    AA2AR_HUMAN
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 NCBI Summary for ADORA2a    This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]
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 UniProt Comments for ADORA2a    ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: integral to plasma membrane; membrane; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled; enzyme binding; identical protein binding; protein binding

Biological Process: adenosine receptor signaling pathway; adenylate cyclase activation; apoptosis; blood circulation; blood coagulation; cAMP biosynthetic process; cell-cell signaling; cellular defense response; cellular protein metabolic process; central nervous system development; G-protein signaling, coupled to cAMP nucleotide second messenger; inflammatory response; nerve growth factor receptor signaling pathway; phagocytosis; sensory perception; transmembrane receptor protein tyrosine kinase signaling pathway
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 Research Articles on ADORA2a    1. This study found a relation between polymorphisms of adenosine A2A and the caffeine effects on the attentional domains of Orienting and Executive control.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADORA2a elisa kitPathways associated with ADORA2a elisa kit
 Reference Product  PubMed Publications
 ADCY9 elisa kit  >1 publications with ADORA2a and ADCY9
 Products by Pathway  Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with ADORA2a elisa kitOrgans/Tissues associated with ADORA2a elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >21 publications with ADORA2a and Inflammation
 Genetic Predisposition to Disease Antibodies  >19 publications with ADORA2a and Genetic Predisposition to Disease
 Disease Models, Animal Antibodies  >12 publications with ADORA2a and Disease Models, Animal
 Anxiety Disorders Antibodies  >11 publications with ADORA2a and Anxiety Disorders
 Movement Disorders Antibodies  >9 publications with ADORA2a and Movement Disorders
 Panic Disorder Antibodies  >7 publications with ADORA2a and Panic Disorder
 Arthritis, Rheumatoid Antibodies  >4 publications with ADORA2a and Arthritis, Rheumatoid
 Neurobehavioral Manifestations Antibodies  >3 publications with ADORA2a and Neurobehavioral Manifestations
 Heart Diseases Antibodies  >3 publications with ADORA2a and Heart Diseases
 Poisoning Antibodies  >3 publications with ADORA2a and Poisoning
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >34 publications with ADORA2a and Brain
 Blood Antibodies  >26 publications with ADORA2a and Blood
 Vascular Antibodies  >11 publications with ADORA2a and Vascular
 Lung Antibodies  >5 publications with ADORA2a and Lung
 Eye Antibodies  >4 publications with ADORA2a and Eye
 Heart Antibodies  >3 publications with ADORA2a and Heart
 Liver Antibodies  >3 publications with ADORA2a and Liver
 Embryonic Tissue Antibodies  >2 publications with ADORA2a and Embryonic Tissue
 Intestine Antibodies  >1 publications with ADORA2a and Intestine
 Stomach Antibodies  >1 publications with ADORA2a and Stomach
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