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ADORA2b elisa kit :: Human Adenosine A2b Receptor (ADORA2b) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS452726
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Adenosine A2b Receptor (ADORA2b), ELISA Kit

★Popular Item★
 Also Known As   

Human Adenosine A2b Receptor (ADORA2b) ELISA Kit

 Product Gene Name   

ADORA2b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    M97759 mRNA
 3D Structure    ModBase 3D Structure for P29275
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADORA2b. No significant cross-reactivity or interference between ADORA2b and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.061ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADORA2b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADORA2b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADORA2b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADORA2b purchase    MBS452726 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine A2b Receptor (ADORA2b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ADORA2b, with an estimated sensitivity of 0.061ng/mL. The ELISA analytical biochemical technique of the MBS452726 kit is based on ADORA2b antibody-ADORA2b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ADORA2b elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADORA2b in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADORA2b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADORA2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADORA2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADORA2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADORA2b elisa kit    ADORA2b elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS452726. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADORA2b. It may not necessarily be applicable to this product.
 NCBI GI #    4501951
 NCBI GeneID    136
 NCBI Accession #    NP_000667.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000676.2 [Other Products]
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 UniProt Primary Accession #    P29275 [Other Products]
 UniProt Related Accession #    P29275 [Other Products]
 Molecular Weight    36,333 Da
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 NCBI Official Full Name    adenosine receptor A2b
 NCBI Official Synonym Full Names    adenosine A2b receptor
 NCBI Official Symbol    ADORA2B [Similar Products]
 NCBI Official Synonym Symbols   
ADORA2
[Similar Products]
 NCBI Protein Information    adenosine receptor A2b
 UniProt Protein Name    Adenosine receptor A2b
 Protein Family    Adenosine receptor
 UniProt Gene Name    ADORA2B [Similar Products]
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 NCBI Summary for ADORA2b    This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADORA2b    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
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 Research Articles on ADORA2b    1. erythrocyte AMP-activated protein kinase was activated in humans at high altitude. It is a key protein functioning downstream of the A2B adenosine receptor, phosphorylating and activating BPG mutase and thus inducing 2,3-BPG production and O2 release from erythrocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADORA2b elisa kitPathways associated with ADORA2b elisa kit
 Reference Product  PubMed Publications
 ADCY5 elisa kit  >1 publications with ADORA2b and ADCY5
 ADCY6 elisa kit  >1 publications with ADORA2b and ADCY6
 ADCY9 elisa kit  >1 publications with ADORA2b and ADCY9
 Products by Pathway  Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with ADORA2b elisa kitOrgans/Tissues associated with ADORA2b elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >22 publications with ADORA2b and Inflammation
 Cardiovascular Diseases Antibodies  >15 publications with ADORA2b and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >13 publications with ADORA2b and Disease Models, Animal
 Heart Diseases Antibodies  >8 publications with ADORA2b and Heart Diseases
 Nervous System Diseases Antibodies  >7 publications with ADORA2b and Nervous System Diseases
 Hypertension Antibodies  >7 publications with ADORA2b and Hypertension
 Lung Injury Antibodies  >6 publications with ADORA2b and Lung Injury
 Kidney Diseases Antibodies  >5 publications with ADORA2b and Kidney Diseases
 Edema Antibodies  >3 publications with ADORA2b and Edema
 Neoplasm Metastasis Antibodies  >3 publications with ADORA2b and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with ADORA2b and Blood
 Lung Antibodies  >10 publications with ADORA2b and Lung
 Heart Antibodies  >9 publications with ADORA2b and Heart
 Brain Antibodies  >6 publications with ADORA2b and Brain
 Intestine Antibodies  >4 publications with ADORA2b and Intestine
 Eye Antibodies  >3 publications with ADORA2b and Eye
 Stomach Antibodies  >2 publications with ADORA2b and Stomach
 Placenta Antibodies  >2 publications with ADORA2b and Placenta
 Mouth Antibodies  >2 publications with ADORA2b and Mouth
 Ascites Antibodies  >1 publications with ADORA2b and Ascites
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