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AK7 elisa kit :: Human Adenylate kinase 7 (AK7) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7239878
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Adenylate kinase 7 (AK7), ELISA Kit

★Popular Item★
 Also Known As   

Human Adenylate kinase 7 (AK7) ELISA Kit

 Product Gene Name   

AK7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    615364
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AK7. No significant cross-reactivity or interference between AK7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK7 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AK7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AK7 purchase    MBS7239878 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenylate kinase 7 (AK7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AK7, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7239878 kit is based on AK7 antibody-AK7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AK7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AK7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AK7 elisa kit

   Intended Uses: This AK7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AK7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: AK7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AK7 antibody and an AK7-HRP conjugate. The assay sample and buffer are incubated together with AK7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK7 concentration since AK7 from samples and AK7-HRP conjugate compete for the anti-AK7 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK7 from the sample, fewer sites are left to bind AK7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK7 concentration in each sample is interpolated from this standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of AK7 elisa kit    AK7 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7239878. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AK7. It may not necessarily be applicable to this product.
 NCBI GI #    148727333
 NCBI GeneID    122481
 NCBI Accession #    NP_689540.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_152327.3 [Other Products]
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 UniProt Secondary Accession #    Q8IYP6 [Other Products]
 UniProt Related Accession #    Q96M32 [Other Products]
 Molecular Weight    82,658 Da
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 NCBI Official Full Name    adenylate kinase 7
 NCBI Official Synonym Full Names    adenylate kinase 7
 NCBI Official Symbol    AK7 [Similar Products]
 NCBI Official Synonym Symbols   
AK 7
[Similar Products]
 NCBI Protein Information    adenylate kinase 7; putative adenylate kinase 7; ATP-AMP transphosphorylase 7
 UniProt Protein Name    Adenylate kinase 7
 UniProt Synonym Protein Names   
ATP-AMP transphosphorylase 7
 Protein Family    Adenylate kinase
 UniProt Gene Name    AK7 [Similar Products]
 UniProt Synonym Gene Names    AK 7 [Similar Products]
 UniProt Entry Name    KAD7_HUMAN
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 UniProt Comments for AK7    AK7: Adenylate kinase involved in maintaining ciliary structure and function. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP.

Protein type: EC 2.7.4.3; Nucleotide Metabolism - purine; EC 2.7.4.6; Kinase, other

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; cytidylate kinase activity; ATP binding

Biological Process: inflammatory response to antigenic stimulus; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; spermatogenesis; brain development; axoneme biogenesis
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 Research Articles on AK7    1. AK7 may be involved in the development of Primary ciliary dyskinesia (PCD).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AK7 elisa kitPathways associated with AK7 elisa kit
 Reference Product  PubMed Publications
 NME5 elisa kit  >1 publications with AK7 and NME5
 NME7 elisa kit  >1 publications with AK7 and NME7
 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
Diseases associated with AK7 elisa kitOrgans/Tissues associated with AK7 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >4 publications with AK7 and Cardiovascular Diseases
 Congenital Abnormalities Antibodies  >4 publications with AK7 and Congenital Abnormalities
 Cardiovascular Abnormalities Antibodies  >3 publications with AK7 and Cardiovascular Abnormalities
 Heart Defects, Congenital Antibodies  >3 publications with AK7 and Heart Defects, Congenital
 Nervous System Diseases Antibodies  >2 publications with AK7 and Nervous System Diseases
 Disease Models, Animal Antibodies  >2 publications with AK7 and Disease Models, Animal
 Anemia Antibodies  >1 publications with AK7 and Anemia
 Brain Diseases Antibodies  >1 publications with AK7 and Brain Diseases
 Genital Diseases, Male Antibodies  >1 publications with AK7 and Genital Diseases, Male
 Inflammation Antibodies  >1 publications with AK7 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >3 publications with AK7 and Skin
 Spleen Antibodies  >3 publications with AK7 and Spleen
 Bone Antibodies  >2 publications with AK7 and Bone
 Muscle Antibodies  >2 publications with AK7 and Muscle
 Embryonic Tissue Antibodies  >2 publications with AK7 and Embryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with AK7 and Uncharacterized Tissue
 Brain Antibodies  >1 publications with AK7 and Brain
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