View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List ACTH elisa kit :: Human Adrenocorticotropic Hormone (ACTH) ELISA Kit Catalog # MBS454423 Unit / Price 48 Tests / $400 +1 FREE 8GB USB 96 Tests / $530 +1 FREE 8GB USB 5x96 Tests / $2,395 +2 FREE 8GB USB 10x96 Tests / $4,675 +5 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Adrenocorticotropic Hormone (ACTH), ELISA Kit Full Product Name Human Adrenocorticotropic Hormone (ACTH) ELISA Kit Product Gene Name ACTH elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of ACTH. No significant cross-reactivity or interference between ACTH and analogues was observed. Samples Serum, plasma and other biological fluids. Assay Type Competitive inhibition Detection Range 15.625-1000pg/mL Sensitivity 5.24pg/mL Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACTH were tested 20 times on one plate, respectively. Intra-Assay: CV<10% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACTH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12% Preparation and Storage For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of ACTH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for ACTH purchase MBS454423 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adrenocorticotropic Hormone (ACTH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTH. The ELISA analytical biochemical technique of the MBS454423 kit is based on ACTH antibody-ACTH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for ACTH elisa kit Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ACTH in serum, plasma and other biological fluids.Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ACTH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ACTH and unlabeled ACTH (Standards or samples) with the pre-coated antibody specific to ACTH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ACTH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ACTH in the sample. Sample Manual Insert of MBS454423. NCBI/Uniprot data below describe general gene information for ACTH. It may not necessarily be applicable to this product. NCBI GI # 191659 NCBI GeneID 18976 UniProt Secondary Accession # P01200; Q544U4[Other Products] UniProt Related Accession # P01193[Other Products] Molecular Weight 26,707 Da TOP NCBI Official Full Name adrenocorticotropic hormone, partial NCBI Official Synonym Full Names pro-opiomelanocortin-alpha NCBI Official Symbol Pomc [Similar Products] NCBI Official Synonym Symbols BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH [Similar Products] NCBI Protein Information pro-opiomelanocortin UniProt Protein Name Pro-opiomelanocortin UniProt Synonym Protein Names Corticotropin-lipotropin UniProt Gene Name Pomc [Similar Products] UniProt Synonym Gene Names Pomc1; POMC; ACTH; CLIP [Similar Products] TOP NCBI Summary for ACTH This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] TOP UniProt Comments for ACTH POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.Protein type: Secreted; Secreted, signal peptideCellular Component: cytoplasm; extracellular region; extracellular space; secretory granuleMolecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor bindingBiological Process: cell-cell signaling; cellular pigmentation; generation of precursor metabolites and energy; glucose homeostasis; negative regulation of tumor necrosis factor production; neuropeptide signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of appetite; regulation of blood pressure; signal transduction Research Articles on ACTH 1. Epo regulates hypothalamus POMC expression via STAT3 activation. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. 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