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GPT elisa kit :: Human Alanine aminotransferase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2887483
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alanine aminotransferase 1 (GPT), ELISA Kit

★Popular Item★
 Also Known As   

Human Alanine aminotransferase 1 ELISA Kit

 Product Synonym Names    Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT; AAT1; GPT1; 2.6.1.2
 Product Gene Name   

GPT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2887483 Testing Data    Testing Data PDF
 OMIM    138200
 3D Structure    ModBase 3D Structure for P24298
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 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    0.156-10 ng/mL
 Sensitivity    1.56 U/L
 Intra-Assay CV    <=4.3%
 Inter-Assay CV    <=7.6%
 Recovery    99%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPT purchase    MBS2887483 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alanine aminotransferase 1 (GPT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/mL in biological research samples containing GPT, with an estimated sensitivity of 1.56 U/L. The ELISA analytical biochemical technique of the MBS2887483 kit is based on GPT antibody-GPT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPT elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GPT elisa kit    GPT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2887483. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPT. It may not necessarily be applicable to this product.
 NCBI GI #    4885351
 NCBI GeneID    2875
 NCBI Accession #    NP_005300.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005309.2 [Other Products]
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 UniProt Primary Accession #    P24298 [Other Products]
 UniProt Secondary Accession #    P78398; Q93076; B0YJ18; D3DWM7 [Other Products]
 UniProt Related Accession #    P24298 [Other Products]
 Molecular Weight    54,637 Da
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 NCBI Official Full Name    alanine aminotransferase 1
 NCBI Official Synonym Full Names    glutamic-pyruvate transaminase (alanine aminotransferase)
 NCBI Official Symbol    GPT [Similar Products]
 NCBI Official Synonym Symbols   
AAT1; ALT1; GPT1
[Similar Products]
 NCBI Protein Information    alanine aminotransferase 1
 UniProt Protein Name    Alanine aminotransferase 1
 UniProt Synonym Protein Names   
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
 Protein Family    Alanine aminotransferase
 UniProt Gene Name    GPT [Similar Products]
 UniProt Synonym Gene Names    AAT1; GPT1; ALT1; GPT 1 [Similar Products]
 UniProt Entry Name    ALAT1_HUMAN
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 NCBI Summary for GPT    This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
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 UniProt Comments for GPT    GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process
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 Research Articles on GPT    1. ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPT elisa kitPathways associated with GPT elisa kit
 Reference Product  PubMed Publications
 GOT1 elisa kit  >5 publications with GPT and GOT1
 GOT2 elisa kit  >4 publications with GPT and GOT2
 LDHA elisa kit  >1 publications with GPT and LDHA
 LDHB elisa kit  >1 publications with GPT and LDHB
 LDHC elisa kit  >1 publications with GPT and LDHC
 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
Diseases associated with GPT elisa kitOrgans/Tissues associated with GPT elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >937 publications with GPT and Liver Diseases
 Poisoning Antibodies  >383 publications with GPT and Poisoning
 Drug-Induced Liver Injury Antibodies  >361 publications with GPT and Drug-Induced Liver Injury
 Hepatitis Antibodies  >336 publications with GPT and Hepatitis
 Drug-Related Side Effects and Adverse Reactions Antibodies  >295 publications with GPT and Drug-Related Side Effects and Adverse Reactions
 Necrosis Antibodies  >270 publications with GPT and Necrosis
 Nervous System Diseases Antibodies  >216 publications with GPT and Nervous System Diseases
 Liver Neoplasms Antibodies  >174 publications with GPT and Liver Neoplasms
 Immune System Diseases Antibodies  >171 publications with GPT and Immune System Diseases
 Disease Models, Animal Antibodies  >162 publications with GPT and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1816 publications with GPT and Liver
 Kidney Antibodies  >360 publications with GPT and Kidney
 Heart Antibodies  >189 publications with GPT and Heart
 Lung Antibodies  >167 publications with GPT and Lung
 Brain Antibodies  >158 publications with GPT and Brain
 Skin Antibodies  >128 publications with GPT and Skin
 Intestine Antibodies  >89 publications with GPT and Intestine
 Mixed Antibodies  >76 publications with GPT and Mixed
 Stomach Antibodies  >59 publications with GPT and Stomach
 Pancreas Antibodies  >42 publications with GPT and Pancreas
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