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ADH1B elisa kit :: Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7233294
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alcohol dehydrogenase 1B (ADH1B), ELISA Kit

★Popular Item★
 Also Known As   

Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit

 Product Gene Name   

ADH1B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADH1B. No significant cross-reactivity or interference between ADH1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADH1B and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This ADH1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADH1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADH1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADH1B purchase    MBS7233294 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alcohol dehydrogenase 1B (ADH1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing ADH1B, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS7233294 kit is based on ADH1B antibody-ADH1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADH1B elisa kit

   Principle of the assay: ADH1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADH1B antibody and an ADH1B-HRP conjugate. The assay sample and buffer are incubated together with ADH1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH1B concentration since ADH1B from samples and ADH1B-HRP conjugate compete for the anti-ADH1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH1B from the sample, fewer sites are left to bind ADH1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH1B concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADH1B elisa kit    ADH1B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7233294. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADH1B. It may not necessarily be applicable to this product.
 NCBI GI #    77683189
 NCBI GeneID    461396
 NCBI Accession #    NP_001029330.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001034158.1 [Other Products]
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 UniProt Related Accession #    Q5R1W2 [Other Products]
 Molecular Weight    39,826 Da
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 NCBI Official Full Name    alcohol dehydrogenase 1B
 NCBI Official Symbol    ADH1B [Similar Products]
 NCBI Protein Information    alcohol dehydrogenase 1B; aldehyde reductase; alcohol dehydrogenase subunit beta; alcohol dehydrogenase IB (class I), beta polypeptide
 UniProt Protein Name    Alcohol dehydrogenase 1B
 UniProt Synonym Protein Names   
Alcohol dehydrogenase subunit beta
 UniProt Gene Name    ADH1B [Similar Products]
 UniProt Entry Name    ADH1B_PANTR
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADH1B elisa kitDiseases associated with ADH1B elisa kit
 Products by Pathway  Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies  Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
 Disease Name  Pubmed Publications
 Alcoholism Antibodies  >132 publications with ADH1B and Alcoholism
 Genetic Predisposition to Disease Antibodies  >76 publications with ADH1B and Genetic Predisposition to Disease
 Esophageal Neoplasms Antibodies  >35 publications with ADH1B and Esophageal Neoplasms
 Liver Diseases Antibodies  >25 publications with ADH1B and Liver Diseases
 Esophageal Squamous Cell Carcinoma Antibodies  >19 publications with ADH1B and Esophageal Squamous Cell Carcinoma
 Nervous System Diseases Antibodies  >14 publications with ADH1B and Nervous System Diseases
 Colorectal Neoplasms Antibodies  >9 publications with ADH1B and Colorectal Neoplasms
 Central Nervous System Diseases Antibodies  >8 publications with ADH1B and Central Nervous System Diseases
 Poisoning Antibodies  >7 publications with ADH1B and Poisoning
 Brain Diseases Antibodies  >6 publications with ADH1B and Brain Diseases
Organs/Tissues associated with ADH1B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with ADH1B and Skin
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