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AKR1C3 elisa kit :: Human Aldo keto reductase family 1 member C3 (AKR1C3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7220102
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aldo keto reductase family 1 member C3 (AKR1C3), ELISA Kit

 Also Known As   

Human Aldo keto reductase family 1 member C3 (AKR1C3) ELISA Kit

 Product Gene Name   

AKR1C3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    603966
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AKR1C3. No significant cross-reactivity or interference between AKR1C3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR1C3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This AKR1C3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AKR1C3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of AKR1C3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AKR1C3 purchase    MBS7220102 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldo keto reductase family 1 member C3 (AKR1C3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKR1C3, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS7220102 kit is based on AKR1C3 antibody-AKR1C3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKR1C3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKR1C3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AKR1C3 elisa kit

   Principle of the Assay: AKR1C3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR1C3 antibody and an AKR1C3-HRP conjugate. The assay sample and buffer are incubated together with AKR1C3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1C3 concentration since AKR1C3 from samples and AKR1C3-HRP conjugate compete for the anti-AKR1C3 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1C3 from the sample, fewer sites are left to bind AKR1C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1C3 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AKR1C3 elisa kit   
AKR1C3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7220102
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NCBI/Uniprot data below describe general gene information for AKR1C3. It may not necessarily be applicable to this product.
 NCBI GI #    24497583
 NCBI GeneID    8644
 NCBI Accession #    NP_003730.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_003739.5 [Other Products]
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 UniProt Secondary Accession #    Q5T2L1; Q96DJ1; Q96KI8; Q99530; Q9UCX1; Q9UII3; Q9UKL9; A8K2V0; B4DL37 [Other Products]
 UniProt Related Accession #    P42330 [Other Products]
 Molecular Weight    23,364 Da
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 NCBI Official Full Name    aldo-keto reductase family 1 member C3 isoform 1
 NCBI Official Synonym Full Names    aldo-keto reductase family 1, member C3
 NCBI Official Symbol    AKR1C3 [Similar Products]
 NCBI Official Synonym Symbols   
DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
[Similar Products]
 NCBI Protein Information    aldo-keto reductase family 1 member C3; indanol dehydrogenase; prostaglandin F synthase; 3-alpha-HSD type II, brain; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; chlordecone reductase homolog HAKRb; testosterone 17-beta-dehydrogenase 5; 3-alpha hydroxysteroid dehydrogenase, type II; type IIb 3-alpha hydroxysteroid dehydrogenase; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
 UniProt Protein Name    Aldo-keto reductase family 1 member C3
 UniProt Synonym Protein Names   
17-beta-hydroxysteroid dehydrogenase type 5; 17-beta-HSD 5; 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2 (EC:1.1.1.357); 3-alpha-HSD type 2; Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3; DD-3; DD3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase (EC:1.1.1.112); Prostaglandin F synthase (EC:1.1.1.188); PGFS; Testosterone 17-beta-dehydrogenase 5 (EC:1.1.1.239, EC:1.1.1.64); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)
 Protein Family    Aldo-keto reductase family
 UniProt Gene Name    AKR1C3 [Similar Products]
 UniProt Synonym Gene Names    DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS [Similar Products]
 UniProt Entry Name    AK1C3_HUMAN
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 NCBI Summary for AKR1C3    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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 UniProt Comments for AKR1C3    AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; EC 1.1.1.149; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm; intracellular; nucleus; cytosol

Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; retinol dehydrogenase activity; phenanthrene 9,10-monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; testosterone 17-beta-dehydrogenase (NADP+) activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; male gonad development; cyclooxygenase pathway; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; multicellular organismal macromolecule metabolic process; farnesol catabolic process; retinoid metabolic process; regulation of retinoic acid receptor signaling pathway; response to nutrient
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 Research Articles on AKR1C3    1. Data suggest that reduction of daunorubicin/idarubicin is catalyzed by AKR1C3 and contributes to resistance of carcinoma cells to these anthracyclines; expression of AKR1C3 is induced in carcinoma cells following exposure to daunorubicin/idarubicin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AKR1C3 elisa kitDiseases associated with AKR1C3 elisa kit
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Benzo(a)pyrene Metabolism Pathway antibodies  Benzo(a)pyrene Metabolism Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >42 publications with AKR1C3 and Prostatic Neoplasms
 Breast Neoplasms Antibodies  >28 publications with AKR1C3 and Breast Neoplasms
 Adenocarcinoma Antibodies  >15 publications with AKR1C3 and Adenocarcinoma
 Obesity Antibodies  >10 publications with AKR1C3 and Obesity
 Neoplasms, Experimental Antibodies  >8 publications with AKR1C3 and Neoplasms, Experimental
 Endometriosis Antibodies  >7 publications with AKR1C3 and Endometriosis
 Hyperplasia Antibodies  >7 publications with AKR1C3 and Hyperplasia
 Polycystic Ovary Syndrome Antibodies  >6 publications with AKR1C3 and Polycystic Ovary Syndrome
 Carcinoma, Squamous Cell Antibodies  >6 publications with AKR1C3 and Carcinoma, Squamous Cell
 Endometrial Neoplasms Antibodies  >6 publications with AKR1C3 and Endometrial Neoplasms
Organs/Tissues associated with AKR1C3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >85 publications with AKR1C3 and Prostate
 Blood Antibodies  >30 publications with AKR1C3 and Blood
 Liver Antibodies  >26 publications with AKR1C3 and Liver
 Lung Antibodies  >23 publications with AKR1C3 and Lung
 Ovary Antibodies  >17 publications with AKR1C3 and Ovary
 Adipose Tissue Antibodies  >15 publications with AKR1C3 and Adipose Tissue
 Uterus Antibodies  >14 publications with AKR1C3 and Uterus
 Adrenal Gland Antibodies  >12 publications with AKR1C3 and Adrenal Gland
 Connective Tissue Antibodies  >12 publications with AKR1C3 and Connective Tissue
 Kidney Antibodies  >11 publications with AKR1C3 and Kidney
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