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ALP elisa kit :: Human Alkaline Phosphatase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721477
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alkaline Phosphatase (ALP), ELISA Kit

★Popular Item★
 Also Known As   

Human Alkaline Phosphatase ELISA Kit

 Product Gene Name   

ALP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    241510
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ALP. No significant cross-reactivity or interference between ALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    5.0ng/mL 100ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALP purchase    MBS721477 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline Phosphatase (ALP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0ng/mL 100ng/mL in biological research samples containing ALP, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS721477 kit is based on ALP antibody-ALP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALP elisa kit

   Intended Uses: This ALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ALP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALP. Standards or samples are then added to the microtiter plate wells and ALP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALP are added to each well to "sandwich" the ALP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALP concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ALP elisa kit    ALP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721477. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALP. It may not necessarily be applicable to this product.
 NCBI GI #    178462
 NCBI GeneID    249
 NCBI Accession #    AAB59378.1 [Other Products]
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 UniProt Secondary Accession #    O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6 [Other Products]
 UniProt Related Accession #    P05186 [Other Products]
 Molecular Weight    51,045 Da
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 NCBI Official Full Name    alkaline phosphatase
 NCBI Official Synonym Full Names    alkaline phosphatase, liver/bone/kidney
 NCBI Official Symbol    ALPL [Similar Products]
 NCBI Official Synonym Symbols   
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
[Similar Products]
 NCBI Protein Information    alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
 UniProt Protein Name    Alkaline phosphatase, tissue-nonspecific isozyme
 UniProt Synonym Protein Names   
Alkaline phosphatase liver/bone/kidney isozyme
 Protein Family    Alkaline proteinase
 UniProt Gene Name    ALPL [Similar Products]
 UniProt Synonym Gene Names    AP-TNAP; TNSALP [Similar Products]
 UniProt Entry Name    PPBT_HUMAN
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 NCBI Summary for ALP    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
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 UniProt Comments for ALP    ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - folate biosynthesis; Motility/polarity/chemotaxis; EC 3.1.3.1; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular matrix; extracellular space; membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding

Biological Process: response to antibiotic; osteoblast differentiation; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification

Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood
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 Research Articles on ALP    1. ABO locus is a major determinant for serum ALP levels in Chinese Han population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ALP elisa kitDiseases associated with ALP elisa kit
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >12 publications with ALP and Kidney Diseases
 Seizures Antibodies  >10 publications with ALP and Seizures
 Cardiovascular Diseases Antibodies  >9 publications with ALP and Cardiovascular Diseases
 Inflammation Antibodies  >8 publications with ALP and Inflammation
 Pain Antibodies  >7 publications with ALP and Pain
 Calcinosis Antibodies  >5 publications with ALP and Calcinosis
 Liver Diseases Antibodies  >5 publications with ALP and Liver Diseases
 Necrosis Antibodies  >4 publications with ALP and Necrosis
 Hypertension Antibodies  >4 publications with ALP and Hypertension
 Hypertrophy Antibodies  >4 publications with ALP and Hypertrophy
Organs/Tissues associated with ALP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >166 publications with ALP and Bone
 Blood Antibodies  >52 publications with ALP and Blood
 Kidney Antibodies  >41 publications with ALP and Kidney
 Liver Antibodies  >39 publications with ALP and Liver
 Connective Tissue Antibodies  >35 publications with ALP and Connective Tissue
 Mouth Antibodies  >14 publications with ALP and Mouth
 Adipose Tissue Antibodies  >14 publications with ALP and Adipose Tissue
 Brain Antibodies  >14 publications with ALP and Brain
 Skin Antibodies  >7 publications with ALP and Skin
 Embryonic Tissue Antibodies  >7 publications with ALP and Embryonic Tissue
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