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alpha1ACT elisa kit :: Human Alpha-1-Antichymotrypsin (alpha1ACT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267255
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
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 Product Name   

Alpha-1-Antichymotrypsin (alpha1ACT), ELISA Kit

★Popular Item★
 Also Known As   

Human Alpha-1-Antichymotrypsin (alpha1ACT) ELISA Kit

 Product Synonym Names    Human Alpha-1-Antichymotrypsin (a1ACT) ELISA Kit
 Product Gene Name   

alpha1ACT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    107280
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha1ACT concentration
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78 ng/ml
 Sensitivity    0.08 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of alpha1ACT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for alpha1ACT purchase    MBS267255 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-1-Antichymotrypsin (alpha1ACT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78 ng/ml in biological research samples containing alpha1ACT, with an estimated sensitivity of 0.08 ng/ml. The ELISA analytical biochemical technique of the MBS267255 kit is based on alpha1ACT antibody-alpha1ACT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha1ACT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha1ACT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha1ACT concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for alpha1ACT elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human alpha1ACT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of alpha1ACT elisa kit    alpha1ACT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS267255. Click to request current manual
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NCBI/Uniprot data below describe general gene information for alpha1ACT. It may not necessarily be applicable to this product.
 NCBI GI #    50659080
 NCBI GeneID    12
 NCBI Accession #    NP_001076.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001085.4 [Other Products]
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 UniProt Secondary Accession #    Q13703; Q2TU87; Q2TU88; Q59GP9; Q6LBY8; Q6LDT7; Q6NSC9; Q8N177; Q96DW8; Q9UC47; B3KVQ7 [Other Products]
 UniProt Related Accession #    P01011 [Other Products]
 Molecular Weight    10,717 Da
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 NCBI Official Full Name    alpha-1-antichymotrypsin
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
 NCBI Official Symbol    SERPINA3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    alpha-1-antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3
 UniProt Protein Name    Alpha-1-antichymotrypsin
 UniProt Synonym Protein Names   
Cell growth-inhibiting gene 24/25 protein; Serpin A3Cleaved into the following chain:Alpha-1-antichymotrypsin His-Pro-less
 UniProt Gene Name    SERPINA3 [Similar Products]
 UniProt Synonym Gene Names    AACT; GIG24; GIG25; ACT [Similar Products]
 UniProt Entry Name    AACT_HUMAN
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 NCBI Summary for alpha1ACT    The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for alpha1ACT    SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space; extracellular region; intracellular; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; DNA binding

Biological Process: regulation of lipid metabolic process; maintenance of gastrointestinal epithelium; acute-phase response; inflammatory response
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 Research Articles on alpha1ACT    1. ACT-17 variation presents a risk factor for Alzheimer's disease in the worldwide population, especially in the Caucasian population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with alpha1ACT elisa kitDiseases associated with alpha1ACT elisa kit
 Products by Pathway  Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with alpha1ACT and Neoplasms
 Inflammation Antibodies  >13 publications with alpha1ACT and Inflammation
 Cardiovascular Diseases Antibodies  >12 publications with alpha1ACT and Cardiovascular Diseases
 Mental Disorders Antibodies  >5 publications with alpha1ACT and Mental Disorders
 Hypertension Antibodies  >4 publications with alpha1ACT and Hypertension
 Necrosis Antibodies  >4 publications with alpha1ACT and Necrosis
 Liver Diseases Antibodies  >4 publications with alpha1ACT and Liver Diseases
 Heart Diseases Antibodies  >3 publications with alpha1ACT and Heart Diseases
 Heart Failure Antibodies  >1 publications with alpha1ACT and Heart Failure
 Seizures Antibodies  >1 publications with alpha1ACT and Seizures
Organs/Tissues associated with alpha1ACT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with alpha1ACT and Brain
 Liver Antibodies  >7 publications with alpha1ACT and Liver
 Uterus Antibodies  >4 publications with alpha1ACT and Uterus
 Lung Antibodies  >2 publications with alpha1ACT and Lung
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