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alpha2 MG elisa kit :: Human Alpha-2-Macroglobulin (alpha2 MG) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265387
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
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 Product Name   

Alpha-2-Macroglobulin (alpha2 MG), ELISA Kit

★Popular Item★
 Also Known As   

Human Alpha-2-Macroglobulin (alpha2 MG) ELISA Kit

 Product Synonym Names    Human Alpha-2-Macroglobulin (a2 MG)  ELISA Kit
 Product Gene Name   

alpha2 MG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    103950
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha2 MG concentration
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78 ng/ml
 Sensitivity    0.1 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of alpha2 MG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for alpha2 MG purchase    MBS265387 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-2-Macroglobulin (alpha2 MG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78 ng/ml in biological research samples containing alpha2 MG, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS265387 kit is based on alpha2 MG antibody-alpha2 MG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha2 MG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha2 MG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alpha2 MG concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for alpha2 MG elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human alpha2 MG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of alpha2 MG elisa kit    alpha2 MG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS265387. Click to request current manual
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NCBI/Uniprot data below describe general gene information for alpha2 MG. It may not necessarily be applicable to this product.
 NCBI GI #    66932947
 NCBI GeneID    2
 NCBI Accession #    NP_000005.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000014.4 [Other Products]
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 UniProt Secondary Accession #    Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97 [Other Products]
 UniProt Related Accession #    P01023 [Other Products]
 Molecular Weight    163,291 Da
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 NCBI Official Full Name    alpha-2-macroglobulin
 NCBI Official Synonym Full Names    alpha-2-macroglobulin
 NCBI Official Symbol    A2M [Similar Products]
 NCBI Official Synonym Symbols   
A2MD; CPAMD5; FWP007; S863-7
[Similar Products]
 NCBI Protein Information    alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 UniProt Protein Name    Alpha-2-macroglobulin
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 UniProt Gene Name    A2M [Similar Products]
 UniProt Synonym Gene Names    CPAMD5; FWP007; Alpha-2-M [Similar Products]
 UniProt Entry Name    A2MG_HUMAN
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 NCBI Summary for alpha2 MG    Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
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 UniProt Comments for alpha2 MG    A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
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 Research Articles on alpha2 MG    1. genetic variation within the A2M gene influences CSF Ab42 levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with alpha2 MG elisa kitPathways associated with alpha2 MG elisa kit
 Reference Product  PubMed Publications
 APP elisa kit  >15 publications with alpha2 MG and APP
 LRP1 elisa kit  >11 publications with alpha2 MG and LRP1
 ALB elisa kit  >6 publications with alpha2 MG and ALB
 TGFB1 elisa kit  >2 publications with alpha2 MG and TGFB1
 FN1 elisa kit  >1 publications with alpha2 MG and FN1
 PLG elisa kit  >1 publications with alpha2 MG and PLG
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
Diseases associated with alpha2 MG elisa kitOrgans/Tissues associated with alpha2 MG elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >89 publications with alpha2 MG and Nervous System Diseases
 Brain Diseases Antibodies  >85 publications with alpha2 MG and Brain Diseases
 Mental Disorders Antibodies  >79 publications with alpha2 MG and Mental Disorders
 Alzheimer Disease Antibodies  >76 publications with alpha2 MG and Alzheimer Disease
 Neoplasms Antibodies  >31 publications with alpha2 MG and Neoplasms
 Inflammation Antibodies  >28 publications with alpha2 MG and Inflammation
 Necrosis Antibodies  >14 publications with alpha2 MG and Necrosis
 Liver Diseases Antibodies  >12 publications with alpha2 MG and Liver Diseases
 Fibrosis Antibodies  >11 publications with alpha2 MG and Fibrosis
 Lung Diseases Antibodies  >11 publications with alpha2 MG and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >119 publications with alpha2 MG and Blood
 Liver Antibodies  >35 publications with alpha2 MG and Liver
 Brain Antibodies  >29 publications with alpha2 MG and Brain
 Vascular Antibodies  >13 publications with alpha2 MG and Vascular
 Lung Antibodies  >10 publications with alpha2 MG and Lung
 Prostate Antibodies  >9 publications with alpha2 MG and Prostate
 Heart Antibodies  >8 publications with alpha2 MG and Heart
 Uterus Antibodies  >6 publications with alpha2 MG and Uterus
 Placenta Antibodies  >5 publications with alpha2 MG and Placenta
 Embryonic Tissue Antibodies  >5 publications with alpha2 MG and Embryonic Tissue
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