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Pre Alpha 2 MG elisa kit :: Human Alpha 2 Macroglobulin precursor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS730542
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Alpha 2 Macroglobulin precursor (Pre Alpha 2 MG), ELISA Kit

★Popular Item★
 Also Known As   

Human Alpha 2 Macroglobulin precursor ELISA Kit

 Product Gene Name   

Pre Alpha 2 MG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    103950
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PRE-?2 MG. No significant cross-reactivity or interference between PRE-?2 MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRE-?2 MG and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100 umoL/L
 Sensitivity    1.0umoL/L
 Intended Uses    This PRE-?2 MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRE-?2 MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Pre Alpha 2 MG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Pre Alpha 2 MG purchase    MBS730542 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha 2 Macroglobulin precursor (Pre Alpha 2 MG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100 umoL/L in biological research samples containing Pre Alpha 2 MG, with an estimated sensitivity of 1.0umoL/L. The ELISA analytical biochemical technique of the MBS730542 kit is based on Pre Alpha 2 MG antibody-Pre Alpha 2 MG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pre Alpha 2 MG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pre Alpha 2 MG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Pre Alpha 2 MG elisa kit

   Principle of the assay: PRE-?2 MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRE-?2 MG antibody and an PRE-?2 MG-HRP conjugate. The assay sample and buffer are incubated together with PRE-?2 MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRE-?2 MG concentration since PRE-?2 MG from samples and PRE-?2 MG-HRP conjugate compete for the anti-PRE-?2 MG antibody binding site. Since the number of sites is limited, as more sites are occupied by PRE-?2 MG from the sample, fewer sites are left to bind PRE-?2 MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRE-?2 MG concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Pre Alpha 2 MG elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of Pre Alpha 2 MG elisa kit    Pre Alpha 2 MG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS730542. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Pre Alpha 2 MG. It may not necessarily be applicable to this product.
 NCBI GI #    66932947
 NCBI GeneID    2
 NCBI Accession #    NP_000005.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000014.4 [Other Products]
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 UniProt Secondary Accession #    Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97 [Other Products]
 UniProt Related Accession #    P01023 [Other Products]
 Molecular Weight    163,291 Da
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 NCBI Official Full Name    alpha-2-macroglobulin
 NCBI Official Synonym Full Names    alpha-2-macroglobulin
 NCBI Official Symbol    A2M [Similar Products]
 NCBI Official Synonym Symbols   
A2MD; CPAMD5; FWP007; S863-7
[Similar Products]
 NCBI Protein Information    alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 UniProt Protein Name    Alpha-2-macroglobulin
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 UniProt Gene Name    A2M [Similar Products]
 UniProt Synonym Gene Names    CPAMD5; FWP007; Alpha-2-M [Similar Products]
 UniProt Entry Name    A2MG_HUMAN
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 NCBI Summary for Pre Alpha 2 MG    Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Pre Alpha 2 MG    A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; extracellular matrix disassembly; platelet activation; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
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 Research Articles on Pre Alpha 2 MG    1. genetic variation within the A2M gene influences CSF Ab42 levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Pre Alpha 2 MG elisa kitPathways associated with Pre Alpha 2 MG elisa kit
 Reference Product  PubMed Publications
 APP elisa kit  >17 publications with Pre Alpha 2 MG and APP
 LRP1 elisa kit  >11 publications with Pre Alpha 2 MG and LRP1
 ALB elisa kit  >6 publications with Pre Alpha 2 MG and ALB
 APOA1 elisa kit  >3 publications with Pre Alpha 2 MG and APOA1
 PLG elisa kit  >2 publications with Pre Alpha 2 MG and PLG
 TGFB1 elisa kit  >2 publications with Pre Alpha 2 MG and TGFB1
 SERPINA1 elisa kit  >1 publications with Pre Alpha 2 MG and SERPINA1
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
Diseases associated with Pre Alpha 2 MG elisa kitOrgans/Tissues associated with Pre Alpha 2 MG elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >94 publications with Pre Alpha 2 MG and Nervous System Diseases
 Mental Disorders Antibodies  >82 publications with Pre Alpha 2 MG and Mental Disorders
 Alzheimer Disease Antibodies  >79 publications with Pre Alpha 2 MG and Alzheimer Disease
 Inflammation Antibodies  >31 publications with Pre Alpha 2 MG and Inflammation
 Necrosis Antibodies  >14 publications with Pre Alpha 2 MG and Necrosis
 Liver Diseases Antibodies  >14 publications with Pre Alpha 2 MG and Liver Diseases
 Lung Diseases Antibodies  >12 publications with Pre Alpha 2 MG and Lung Diseases
 Disease Models, Animal Antibodies  >11 publications with Pre Alpha 2 MG and Disease Models, Animal
 Fibrosis Antibodies  >11 publications with Pre Alpha 2 MG and Fibrosis
 Carcinoma Antibodies  >8 publications with Pre Alpha 2 MG and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >124 publications with Pre Alpha 2 MG and Blood
 Liver Antibodies  >37 publications with Pre Alpha 2 MG and Liver
 Brain Antibodies  >30 publications with Pre Alpha 2 MG and Brain
 Vascular Antibodies  >13 publications with Pre Alpha 2 MG and Vascular
 Lung Antibodies  >13 publications with Pre Alpha 2 MG and Lung
 Heart Antibodies  >9 publications with Pre Alpha 2 MG and Heart
 Prostate Antibodies  >9 publications with Pre Alpha 2 MG and Prostate
 Uterus Antibodies  >6 publications with Pre Alpha 2 MG and Uterus
 Embryonic Tissue Antibodies  >6 publications with Pre Alpha 2 MG and Embryonic Tissue
 Placenta Antibodies  >5 publications with Pre Alpha 2 MG and Placenta
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