View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Alpha GAL elisa kit :: Human Alpha Galactosidase ELISA Kit Catalog # MBS724596 Unit / Price Please Inquire Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Alpha Galactosidase, ELISA Kit Full Product Name Human Alpha Galactosidase ELISA Kit Product Gene Name Alpha GAL elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS724596 COA COA PDF Species Reactivity Human Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Competitive Sensitivity 1.0 ng/mL. Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Alpha GAL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Alpha GAL purchase MBS724596 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha Galactosidase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Alpha GAL. The ELISA analytical biochemical technique of the MBS724596 kit is based on Alpha GAL antibody-Alpha GAL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Alpha GAL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Alpha GAL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Alpha GAL elisa kit Intended Uses: This alpha-Galactosidase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alpha-Galactosidase. This ELISA kit for research use only!Principle of the Assay||Alpha-Galactosidase ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-alpha-Galactosidase antibody and an alpha-Galactosidase-HRP conjugate. The assay sample and buffer are incubated together with alpha-Galactosidase-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-Galactosidase concentration since alpha-Galactosidase from samples and alpha-Galactosidase-HRP conjugate compete for the anti-alpha-Galactosidase antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha-Galactosidase from the sample, fewer sites are left to bind alpha-Galactosidase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-Galactosidase concentration in each sample is interpolated from this standard curve. Typical Testing Data/Standard Curve of Alpha GAL elisa kit TOP Sample Manual Insert of MBS724596. NCBI/Uniprot data below describe general gene information for Alpha GAL. It may not necessarily be applicable to this product. NCBI GI # 93204535 TOP NCBI Official Full Name Alpha-galactosidase TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP