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FTO elisa kit :: Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2887853
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alpha-ketoglutarate-dependent dioxygenase FTO, ELISA Kit

 Also Known As   

Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit

 Product Synonym Names    Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO; KIAA1752; 1.14.11.-
 Product Gene Name   

FTO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601665
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 Species Reactivity    Human
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 Specificity    This assay recognizes recombinant and natural human Alpha-ketoglutarate-dependent dioxygenase FTO. No significant cross-reactivity or interference was observed.
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 Samples    Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
 Detection Range    0.31 ng/mL -20.0 ng/mL.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FTO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FTO purchase    MBS2887853 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-ketoglutarate-dependent dioxygenase FTO, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.31 ng/mL -20.0 ng/mL in biological research samples containing FTO. The ELISA analytical biochemical technique of the MBS2887853 kit is based on FTO antibody-FTO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FTO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FTO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FTO elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Alpha-ketoglutarate-dependent dioxygenase FTO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutarate-dependent dioxygenase FTO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-ketoglutarate-dependent dioxygenase FTO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-ketoglutarate-dependent dioxygenase FTO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Alpha-ketoglutarate-dependent dioxygenase FTO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of FTO elisa kit    FTO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2887853. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FTO. It may not necessarily be applicable to this product.
 NCBI GI #    122937263
 NCBI GeneID    79068
 NCBI Accession #    NP_001073901.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001080432.2 [Other Products]
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 UniProt Secondary Accession #    Q0P676; Q7Z785; A2RUH1; B2RNS0 [Other Products]
 UniProt Related Accession #    Q9C0B1 [Other Products]
 Molecular Weight    12,218 Da
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 NCBI Official Full Name    alpha-ketoglutarate-dependent dioxygenase FTO
 NCBI Official Synonym Full Names    fat mass and obesity associated
 NCBI Official Symbol    FTO [Similar Products]
 NCBI Official Synonym Symbols   
GDFD; ALKBH9; BMIQ14
[Similar Products]
 NCBI Protein Information    alpha-ketoglutarate-dependent dioxygenase FTO
 UniProt Protein Name    Alpha-ketoglutarate-dependent dioxygenase FTO
 UniProt Synonym Protein Names   
Fat mass and obesity-associated protein
 Protein Family    Alpha-ketoglutarate-dependent dioxygenase
 UniProt Gene Name    FTO [Similar Products]
 UniProt Synonym Gene Names    KIAA1752 [Similar Products]
 UniProt Entry Name    FTO_HUMAN
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 NCBI Summary for FTO    This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
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 UniProt Comments for FTO    FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nuclear speck; nucleus

Molecular Function: DNA-N1-methyladenine dioxygenase activity; ferrous iron binding

Biological Process: DNA dealkylation; RNA repair

Disease: Body Mass Index Quantitative Trait Locus 14; Growth Retardation, Developmental Delay, Coarse Facies, And Early Death
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 Research Articles on FTO    1. The effects of exercise combined with dietary intervention on obesity were associated with the FTO rs9939609 polymorphism in Chinese adolescents and children.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FTO elisa kitDiseases associated with FTO elisa kit
 Reference Product  PubMed Publications
 MC4R elisa kit  >194 publications with FTO and MC4R
 TMEM18 elisa kit  >80 publications with FTO and TMEM18
 TCF7L2 elisa kit  >79 publications with FTO and TCF7L2
 CDKAL1 elisa kit  >61 publications with FTO and CDKAL1
 SLC30A8 elisa kit  >52 publications with FTO and SLC30A8
 IGF2BP2 elisa kit  >43 publications with FTO and IGF2BP2
 NPY elisa kit  >9 publications with FTO and NPY
 IL6 elisa kit  >5 publications with FTO and IL6
 UCP1 elisa kit  >4 publications with FTO and UCP1
 Disease Name  Pubmed Publications
 Obesity Antibodies  >970 publications with FTO and Obesity
 Body Weight Antibodies  >716 publications with FTO and Body Weight
 Diabetes Mellitus, Type 2 Antibodies  >182 publications with FTO and Diabetes Mellitus, Type 2
 Weight Gain Antibodies  >90 publications with FTO and Weight Gain
 Weight Loss Antibodies  >65 publications with FTO and Weight Loss
 Pediatric Obesity Antibodies  >44 publications with FTO and Pediatric Obesity
 Birth Weight Antibodies  >44 publications with FTO and Birth Weight
 Inflammation Antibodies  >28 publications with FTO and Inflammation
 Neoplasms, Experimental Antibodies  >27 publications with FTO and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >27 publications with FTO and Nervous System Diseases
Organs/Tissues associated with FTO elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >105 publications with FTO and Brain
 Connective Tissue Antibodies  >97 publications with FTO and Connective Tissue
 Heart Antibodies  >77 publications with FTO and Heart
 Liver Antibodies  >76 publications with FTO and Liver
 Muscle Antibodies  >49 publications with FTO and Muscle
 Ovary Antibodies  >27 publications with FTO and Ovary
 Pancreas Antibodies  >21 publications with FTO and Pancreas
 Kidney Antibodies  >19 publications with FTO and Kidney
 Lung Antibodies  >10 publications with FTO and Lung
 Embryonic Tissue Antibodies  >10 publications with FTO and Embryonic Tissue
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