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Amylin elisa kit :: Human Amylin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721421
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Amylin, ELISA Kit

★Popular Item★
 Also Known As   

Human Amylin ELISA Kit

 Product Gene Name   

Amylin elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147940
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This Amy ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Amy. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Amylin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Amylin purchase    MBS721421 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amylin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing Amylin, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS721421 kit is based on Amylin antibody-Amylin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Amylin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Amylin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Amylin elisa kit

   Principle of the Assay: Amy ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Amy antibody and an Amy-HRP conjugate. The assay sample and buffer are incubated together with Amy-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Amy concentration since Amy from samples and Amy-HRP conjugate compete for the anti-Amy antibody binding site. Since the number of sites is limited, as more sites are occupied by Amy from the sample, fewer sites are left to bind Amy-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Amy concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Amylin elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of Amylin elisa kit    Amylin elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721421. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Amylin. It may not necessarily be applicable to this product.
 NCBI GI #    457131
 NCBI GeneID    3375
 NCBI Accession #    AAA35524.1 [Other Products]
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 UniProt Secondary Accession #    Q0ZD87; Q14598 [Other Products]
 UniProt Related Accession #    P10997 [Other Products]
 Molecular Weight    9,806 Da
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 NCBI Official Full Name    amylin, partial
 NCBI Official Synonym Full Names    islet amyloid polypeptide
 NCBI Official Symbol    IAPP [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin)
 UniProt Protein Name    Islet amyloid polypeptide
 UniProt Synonym Protein Names   
Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide
 UniProt Gene Name    IAPP [Similar Products]
 UniProt Synonym Gene Names    DAP [Similar Products]
 UniProt Entry Name    IAPP_HUMAN
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 NCBI Summary for Amylin    Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]
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 UniProt Comments for Amylin    IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: identical protein binding; hormone activity; receptor binding

Biological Process: negative regulation of cell differentiation; cell-cell signaling; eating behavior; apoptosis; signal transduction; negative regulation of bone resorption; sensory perception of pain; endocrine pancreas development
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 Research Articles on Amylin    1. In the critically ill amylin, concentrations are not elevated and not associated with feed intolerance
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Amylin elisa kitPathways associated with Amylin elisa kit
 Reference Product  PubMed Publications
 INS elisa kit  >40 publications with Amylin and INS
 SCT elisa kit  >30 publications with Amylin and SCT
 RAMP3 elisa kit  >18 publications with Amylin and RAMP3
 TTR elisa kit  >4 publications with Amylin and TTR
 ADM elisa kit  >3 publications with Amylin and ADM
 GCG elisa kit  >3 publications with Amylin and GCG
 CALCR elisa kit  >2 publications with Amylin and CALCR
 CALCA elisa kit  >1 publications with Amylin and CALCA
 Products by Pathway  Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Calcitonin-like Ligand Receptors Pathway antibodies  Calcitonin-like Ligand Receptors Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
Diseases associated with Amylin elisa kitOrgans/Tissues associated with Amylin elisa kit
 Disease Name  Pubmed Publications
 Diabetes Mellitus Antibodies  >900 publications with Amylin and Diabetes Mellitus
 Diabetes Mellitus, Type 2 Antibodies  >586 publications with Amylin and Diabetes Mellitus, Type 2
 Insulin Resistance Antibodies  >211 publications with Amylin and Insulin Resistance
 Hyperglycemia Antibodies  >170 publications with Amylin and Hyperglycemia
 Hyperinsulinism Antibodies  >118 publications with Amylin and Hyperinsulinism
 Disease Models, Animal Antibodies  >113 publications with Amylin and Disease Models, Animal
 Diabetes Mellitus, Experimental Antibodies  >86 publications with Amylin and Diabetes Mellitus, Experimental
 Inflammation Antibodies  >42 publications with Amylin and Inflammation
 Hypertension Antibodies  >34 publications with Amylin and Hypertension
 Kidney Diseases Antibodies  >19 publications with Amylin and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >787 publications with Amylin and Pancreas
 Connective Tissue Antibodies  >41 publications with Amylin and Connective Tissue
 Mixed Antibodies  >36 publications with Amylin and Mixed
 Testis Antibodies  >1 publications with Amylin and Testis
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