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ANGPT2 elisa kit :: Human Angiopoietin-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355302
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiopoietin-2 (ANGPT2), ELISA Kit

 Also Known As   

ANGII Human ELISA Kit

 Product Synonym Names    Human Angiopoietin-2
 Product Gene Name   

ANGPT2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 8; NC_000008.10 (6357172..6420784, complement). Location: 8p23.1
 OMIM    601922
 3D Structure    ModBase 3D Structure for O15123
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 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    8 pg/ml-150 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 6 months.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANGPT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANGPT2 purchase    MBS355302 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiopoietin-2 (ANGPT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 8 pg/ml-150 pg/ml in biological research samples containing ANGPT2. The ELISA analytical biochemical technique of the MBS355302 kit is based on ANGPT2 antibody-ANGPT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ANGPT2 elisa kit

   Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-ANG II antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-ANG II antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG II amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG II can be calculated.

Background: Angiopoietins are protein growth factors that promote angiogenesis, the formation of blood vessels from pre-existing blood vessels. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. ANG II is a naturally occurring antagonist of angiopoietin-1 (ANGPT1) that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. ANG II is a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema. It plays a critical role in inducing tumor cell infiltration, and that this invasive phenotype is caused by activation of MMP2.
 Applications Tested/Suitable for ANGPT2 elisa kit   

Quantitative sELISA (EIA)

 Application Notes for ANGPT2 elisa kit    For quantitative detection of ANGII in Human serum, plasma, urine, cell culture supernatant or tissue samples.
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 Typical Testing Data/Standard Curve (for reference only) of ANGPT2 elisa kit    ANGPT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355302. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANGPT2. It may not necessarily be applicable to this product.
 NCBI GI #    50082538
 NCBI GeneID    285
 NCBI Accession #    AAT69979.1 [Other Products]
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 UniProt Primary Accession #    O15123 [Other Products]
 UniProt Secondary Accession #    Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7 [Other Products]
 UniProt Related Accession #    O15123 [Other Products]
 Molecular Weight    56,919 Da [Similar Products]
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 NCBI Official Full Name    angiopoietin-2
 NCBI Official Synonym Full Names    angiopoietin 2
 NCBI Official Symbol    ANGPT2 [Similar Products]
 NCBI Official Synonym Symbols   
ANG2; AGPT2
[Similar Products]
 NCBI Protein Information    angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
 UniProt Protein Name    Angiopoietin-2
 Protein Family    Angiopoietin
 UniProt Gene Name    ANGPT2 [Similar Products]
 UniProt Synonym Gene Names    ANG-2 [Similar Products]
 UniProt Entry Name    ANGP2_HUMAN
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 NCBI Summary for ANGPT2    The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ANGPT2    Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Ref.1 Ref.9 Ref.10 Ref.11

Subunit structure: Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. Ref.1 Ref.8 Ref.9 Ref.11

Subcellular location: Secreted.

Domain: The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.
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 Research Articles on ANGPT2    1. Circulating Ang-2 in myeloma patients significantly correlates to factors of disease burden and prognosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANGPT2 elisa kitPathways associated with ANGPT2 elisa kit
 Reference Product  PubMed Publications
 ANGPT1 elisa kit  >797 publications with ANGPT2 and ANGPT1
 VEGFA elisa kit  >113 publications with ANGPT2 and VEGFA
 TEK elisa kit  >37 publications with ANGPT2 and TEK
 TIE1 elisa kit  >30 publications with ANGPT2 and TIE1
 FOXO1 elisa kit  >7 publications with ANGPT2 and FOXO1
 ETS1 elisa kit  >6 publications with ANGPT2 and ETS1
 CDKN1A elisa kit  >3 publications with ANGPT2 and CDKN1A
 FYN elisa kit  >3 publications with ANGPT2 and FYN
 PIK3CA elisa kit  >1 publications with ANGPT2 and PIK3CA
 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Tie2 Signaling Pathway antibodies  Tie2 Signaling Pathway Diagram
Diseases associated with ANGPT2 elisa kitOrgans/Tissues associated with ANGPT2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >435 publications with ANGPT2 and Neoplasms
 Cardiovascular Diseases Antibodies  >227 publications with ANGPT2 and Cardiovascular Diseases
 Inflammation Antibodies  >189 publications with ANGPT2 and Inflammation
 Disease Models, Animal Antibodies  >141 publications with ANGPT2 and Disease Models, Animal
 Necrosis Antibodies  >96 publications with ANGPT2 and Necrosis
 Neoplasms, Experimental Antibodies  >71 publications with ANGPT2 and Neoplasms, Experimental
 Liver Diseases Antibodies  >69 publications with ANGPT2 and Liver Diseases
 Heart Diseases Antibodies  >69 publications with ANGPT2 and Heart Diseases
 Liver Neoplasms Antibodies  >65 publications with ANGPT2 and Liver Neoplasms
 Hypertension Antibodies  >57 publications with ANGPT2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1179 publications with ANGPT2 and Vascular
 Muscle Antibodies  >162 publications with ANGPT2 and Muscle
 Embryonic Tissue Antibodies  >133 publications with ANGPT2 and Embryonic Tissue
 Brain Antibodies  >122 publications with ANGPT2 and Brain
 Lung Antibodies  >118 publications with ANGPT2 and Lung
 Liver Antibodies  >107 publications with ANGPT2 and Liver
 Kidney Antibodies  >91 publications with ANGPT2 and Kidney
 Eye Antibodies  >85 publications with ANGPT2 and Eye
 Placenta Antibodies  >63 publications with ANGPT2 and Placenta
 Bone Marrow Antibodies  >55 publications with ANGPT2 and Bone Marrow
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