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ACE elisa kit :: Human Angiotensin I Converting Enzyme (ACE) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021446 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $245 +1 FREE 8GB USB
48-Strip-Wells  /  $415 +1 FREE 8GB USB
96-Strip-Wells  /  $555 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,220 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,000 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin I Converting Enzyme (ACE), ELISA Kit

★Popular Item★
 Also Known As   

Angiotensin I Converting Enzyme (ACE) ELISA Kit

 Product Synonym Names    CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
 Product Gene Name   

ACE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    106180
 3D Structure    ModBase 3D Structure for P12821
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE).
No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.39-25ng/mL
 Sensitivity    < 0.16ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACE purchase    MBS2021446 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin I Converting Enzyme (ACE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.39-25ng/mL in biological research samples containing ACE, with an estimated sensitivity of < 0.16ng/mL. The ELISA analytical biochemical technique of the MBS2021446 kit is based on ACE antibody-ACE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ACE elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ACE elisa kit    Enzyme & Kinase; Cardiovascular biology
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 Typical Standard Curve/Testing Data of ACE elisa kit    ACE elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2021446. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ACE. It may not necessarily be applicable to this product.
 NCBI GI #    76057114
 NCBI GeneID    1636
 NCBI Accession #    ABA39229.1 [Other Products]
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 UniProt Primary Accession #    P12821 [Other Products]
 UniProt Secondary Accession #    P22966; Q53YX9; Q59GY8; Q7M4L4; B0LPF0; B4DXI3; E7EU16 [Other Products]
 UniProt Related Accession #    P12821 [Other Products]
 Molecular Weight    78,694 Da
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 NCBI Official Full Name    angiotensin I converting enzyme, partial
 NCBI Official Synonym Full Names    angiotensin I converting enzyme
 NCBI Official Symbol    ACE [Similar Products]
 NCBI Official Synonym Symbols   
DCP; ICH; ACE1; DCP1; CD143; MVCD3
[Similar Products]
 NCBI Protein Information    angiotensin-converting enzyme
 UniProt Protein Name    Angiotensin-converting enzyme
 UniProt Synonym Protein Names   
Dipeptidyl carboxypeptidase I; Kininase II; CD_antigen: CD143
 Protein Family    Acetylcholinesterase
 UniProt Gene Name    ACE [Similar Products]
 UniProt Synonym Gene Names    DCP; DCP1; ACE [Similar Products]
 UniProt Entry Name    ACE_HUMAN
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 NCBI Summary for ACE    This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
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 UniProt Comments for ACE    ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Membrane protein, integral; EC 3.4.15.1

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; lysosome; integral to membrane; extracellular region; plasma membrane; endosome; external side of plasma membrane

Molecular Function: peptidyl-dipeptidase activity; tripeptidyl-peptidase activity; carboxypeptidase activity; metallopeptidase activity; zinc ion binding; mitogen-activated protein kinase kinase binding; drug binding; actin binding; protein binding; endopeptidase activity; bradykinin receptor binding; exopeptidase activity; mitogen-activated protein kinase binding; chloride ion binding

Biological Process: mononuclear cell proliferation; regulation of vasodilation; neutrophil mediated immunity; angiotensin mediated regulation of renal output; regulation of angiotensin metabolic process; arachidonic acid secretion; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; antigen processing and presentation of peptide antigen via MHC class I; regulation of smooth muscle cell migration; cellular protein metabolic process; heart contraction; regulation of vasoconstriction; peptide catabolic process; beta-amyloid metabolic process; regulation of blood pressure; angiotensin catabolic process in blood; hormone catabolic process; blood vessel remodeling; spermatogenesis; kidney development

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 3; Renal Tubular Dysgenesis; Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To
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 Research Articles on ACE    1. ACE (I/D) and FABP2 (Ala54Thr) genes polymorphism and its susceptibility to type 2 diabetes mellitus in the North Indian population, was studied.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ACE elisa kitPathways associated with ACE elisa kit
 Reference Product  PubMed Publications
 ACE2 elisa kit  >496 publications with ACE and ACE2
 AGT elisa kit  >458 publications with ACE and AGT
 SERPINE1 elisa kit  >202 publications with ACE and SERPINE1
 NOS3 elisa kit  >157 publications with ACE and NOS3
 REN elisa kit  >147 publications with ACE and REN
 FOS elisa kit  >42 publications with ACE and FOS
 CTGF elisa kit  >22 publications with ACE and CTGF
 VEGFA elisa kit  >18 publications with ACE and VEGFA
 ATP6AP2 elisa kit  >9 publications with ACE and ATP6AP2
 KNG1 elisa kit  >1 publications with ACE and KNG1
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
Diseases associated with ACE elisa kitOrgans/Tissues associated with ACE elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >13853 publications with ACE and Cardiovascular Diseases
 Hypertension Antibodies  >8959 publications with ACE and Hypertension
 Heart Failure Antibodies  >4664 publications with ACE and Heart Failure
 Kidney Diseases Antibodies  >3498 publications with ACE and Kidney Diseases
 Myocardial Infarction Antibodies  >2582 publications with ACE and Myocardial Infarction
 Coronary Disease Antibodies  >2219 publications with ACE and Coronary Disease
 Stroke Antibodies  >1436 publications with ACE and Stroke
 Renal Insufficiency Antibodies  >1348 publications with ACE and Renal Insufficiency
 Respiratory Tract Diseases Antibodies  >1302 publications with ACE and Respiratory Tract Diseases
 Coronary Artery Disease Antibodies  >1252 publications with ACE and Coronary Artery Disease
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12300 publications with ACE and Blood
 Heart Antibodies  >8369 publications with ACE and Heart
 Vascular Antibodies  >4621 publications with ACE and Vascular
 Kidney Antibodies  >4276 publications with ACE and Kidney
 Muscle Antibodies  >2205 publications with ACE and Muscle
 Lung Antibodies  >1594 publications with ACE and Lung
 Brain Antibodies  >1328 publications with ACE and Brain
 Intestine Antibodies  >277 publications with ACE and Intestine
 Eye Antibodies  >244 publications with ACE and Eye
 Testis Antibodies  >227 publications with ACE and Testis
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