Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ACE II rightarrow Human ACE II  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Park7-DJ-1 Antibody
 • ABCA2 Peptide
 • Cyclooxygenase 1 (COX1) ELISA Kit
 • CD209 Antibody
 • CD4 Antibody
 • 14-3-3 protein epsilon (YWHAE) ELISA Kit
 • FGFR4 Antibody
 • Carcinoembryonic antigen-related cell adhesion molecule 6 (C... Recombinant Protein
 • Epstein-Barr Virus (EBV) Nuclear Antigen Antibody
 • Influenza A Virus H3N2 Shangdong 9-93 Native Protein
 • sAGER ELISA Kit
 • MBP Antibody
 • Protein Tyrosine Phosphatase 1B (PTPN1) Recombinant Protein
 • HEMOGLOBIN (HBA2) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ACE II elisa kit :: Human Angiotensin II Converting Enzyme ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723213
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin II Converting Enzyme (ACE II), ELISA Kit

★Popular Item★
 Also Known As   

Human Angiotensin II Converting Enzyme ELISA Kit

 Product Synonym Names    Human Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Human)
 Product Gene Name   

ACE II elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: X; NC_000023.10 (15579156..15620192, complement). Location: Xp22
 OMIM    300335
 3D Structure    ModBase 3D Structure for Q9BYF1
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0 pg/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACE II elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACE II purchase    MBS723213 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin II Converting Enzyme (ACE II) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing ACE II, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS723213 kit is based on ACE II antibody-ACE II antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACE II antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACE II. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ACE II elisa kit

   Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACE2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACE2. Standards or samples are then added to the microtiter plate wells and ACE2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACE2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACE2 are added to each well to "sandwich" the ACE2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACE2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ACE II elisa kit    Human ELISA Kit
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ACE II elisa kit    ACE II elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS723213. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ACE II. It may not necessarily be applicable to this product.
 NCBI GI #    11225609
 NCBI GeneID    59272
 NCBI Accession #    NP_068576.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021804.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9BYF1 [Other Products]
 UniProt Secondary Accession #    Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6 [Other Products]
 UniProt Related Accession #    Q9BYF1 [Other Products]
 Molecular Weight    92,463 Da
Table BarTOPTable Bar
 NCBI Official Full Name    angiotensin-converting enzyme 2
 NCBI Official Synonym Full Names    angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
 NCBI Official Symbol    ACE2 [Similar Products]
 NCBI Official Synonym Symbols   
ACEH; DKFZp434A014
[Similar Products]
 NCBI Protein Information    angiotensin-converting enzyme 2; OTTHUMP00000022963; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog
 UniProt Protein Name    Angiotensin-converting enzyme 2
 UniProt Synonym Protein Names   
ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15
 UniProt Gene Name    ACE2 [Similar Products]
 UniProt Entry Name    ACE2_HUMAN
Section Bar
 NCBI Summary for ACE II    The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]
Section Bar
 UniProt Comments for ACE II    Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Ref.1 Ref.2 Ref.14

Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.

Cofactor: Binds 1 zinc ion per subunit. Ref.13Binds 1 chloride ion per subunit. Ref.13

Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Ref.1 Ref.2 Ref.3 Ref.13

Subunit structure: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. Ref.11 Ref.14 Ref.17 Ref.20 Ref.22

Subcellular location: Processed angiotensin-converting enzyme 2: Secreted Ref.3. Cell membrane; Single-pass type I membrane protein Ref.3.

Tissue specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. Ref.1 Ref.2 Ref.3 Ref.12 Ref.16 Ref.19

Induction: Up-regulated in failing heart. Ref.1 Ref.2 Ref.3 Ref.13 Ref.15 Ref.19

Post-translational modification: N-glycosylation on Asn-90 may limit SARS infectivity.

Sequence similarities: Belongs to the peptidase M2 family.

Biophysicochemical propertiesKinetic parameters:KM=6.9 µM for angiotensin I Ref.13KM=2 µM for angiotensin IIKM=6.8 µM for apelin-13KM=5.5 µM for dynorphin-13pH dependence:Optimum pH is 6.5 in the presence of 1 M NaCl. Active from pH 6 to 9.
Table BarTOPTable Bar
 Research Articles on ACE II    1. These results suggest that the structural features of the hot spot on hACE2 were among the driving forces for the convergent evolution of NL63 and SARS coronaviruses.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ACE II elisa kitPathways associated with ACE II elisa kit
 Reference Product  PubMed Publications
 AGT elisa kit  >15 publications with ACE II and AGT
 NTS elisa kit  >3 publications with ACE II and NTS
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
Diseases associated with ACE II elisa kitOrgans/Tissues associated with ACE II elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >187 publications with ACE II and Cardiovascular Diseases
 Hypertension Antibodies  >183 publications with ACE II and Hypertension
 Common Cold Antibodies  >65 publications with ACE II and Common Cold
 Kidney Diseases Antibodies  >59 publications with ACE II and Kidney Diseases
 Fibrosis Antibodies  >47 publications with ACE II and Fibrosis
 Heart Failure Antibodies  >43 publications with ACE II and Heart Failure
 Lung Diseases Antibodies  >30 publications with ACE II and Lung Diseases
 Ventricular Remodeling Antibodies  >25 publications with ACE II and Ventricular Remodeling
 Cardiomegaly Antibodies  >23 publications with ACE II and Cardiomegaly
 Inflammation Antibodies  >21 publications with ACE II and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >137 publications with ACE II and Kidney
 Muscle Antibodies  >83 publications with ACE II and Muscle
 Brain Antibodies  >40 publications with ACE II and Brain
 Intestine Antibodies  >13 publications with ACE II and Intestine
 Testis Antibodies  >10 publications with ACE II and Testis
 Placenta Antibodies  >7 publications with ACE II and Placenta
 Mixed Antibodies  >6 publications with ACE II and Mixed
 Eye Antibodies  >4 publications with ACE II and Eye
 Stomach Antibodies  >3 publications with ACE II and Stomach
 Mouth Antibodies  >1 publications with ACE II and Mouth
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions