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ANXA13 elisa kit :: Human Annexin A13 (ANXA13) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7208529
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Annexin A13 (ANXA13), ELISA Kit

★Popular Item★
 Also Known As   

Human Annexin A13 (ANXA13) ELISA Kit

 Product Gene Name   

ANXA13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602573
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ANX-A13. No significant cross-reactivity or interference between ANX-A13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANX-A13 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This ANX-A13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANX-A13. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANXA13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANXA13 purchase    MBS7208529 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Annexin A13 (ANXA13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing ANXA13, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS7208529 kit is based on ANXA13 antibody-ANXA13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANXA13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANXA13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANXA13 elisa kit

   Principle of the assay: ANX-A13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANX-A13 antibody and an ANX-A13-HRP conjugate. The assay sample and buffer are incubated together with ANX-A13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANX-A13 concentration since ANX-A13 from samples and ANX-A13-HRP conjugate compete for the anti-ANX-A13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANX-A13 from the sample, fewer sites are left to bind ANX-A13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANX-A13 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ANXA13 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of ANXA13 elisa kit    ANXA13 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7208529. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANXA13. It may not necessarily be applicable to this product.
 NCBI GI #    281185504
 NCBI GeneID    312
 NCBI Accession #    P27216.3 [Other Products]
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 UniProt Secondary Accession #    Q9BQR5 [Other Products]
 UniProt Related Accession #    P27216 [Other Products]
 Molecular Weight    39,744 Da
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 NCBI Official Full Name    Annexin A13
 NCBI Official Synonym Full Names    annexin A13
 NCBI Official Symbol    ANXA13 [Similar Products]
 NCBI Official Synonym Symbols   
ISA; ANX13
[Similar Products]
 NCBI Protein Information    annexin A13; annexin-13; annexin XIII; intestine-specific annexin; annexin, intestine-specific
 UniProt Protein Name    Annexin A13
 UniProt Synonym Protein Names   
Annexin XIII; Annexin-13; Intestine-specific annexin; ISA
 Protein Family    Annexin
 UniProt Gene Name    ANXA13 [Similar Products]
 UniProt Synonym Gene Names    ANX13; ISA [Similar Products]
 UniProt Entry Name    ANX13_HUMAN
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 NCBI Summary for ANXA13    This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The specific function of this gene has not yet been determined; however, it is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ANXA13    ANXA13: a calcium/phospholipid-binding protein associated with the cell membrane. ANXA13 is the probable common ancestor of the annexin family of proteins. Annexins all bind to phospholipids in a calcium-dependent manner. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleoplasm; extracellular space; basolateral plasma membrane; apical plasma membrane; plasma membrane; lipid raft

Molecular Function: calcium-dependent phospholipid binding; phosphatidylserine binding; phosphatidylcholine binding; calcium ion binding

Biological Process: negative regulation of Golgi to plasma membrane protein transport; positive regulation of Golgi to plasma membrane protein transport; cell differentiation
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 Research Articles on ANXA13    1. Ectopic overexpression of Annexin A13 was likewise sufficient to sensitize malignant breast cancer cells to treatment with Rapamycin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANXA13 elisa kitDiseases associated with ANXA13 elisa kit
 Reference Product  PubMed Publications
 EXT1 elisa kit  >2 publications with ANXA13 and EXT1
 TRPS1 elisa kit  >1 publications with ANXA13 and TRPS1
 CSMD3 elisa kit  >1 publications with ANXA13 and CSMD3
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with ANXA13 and Nervous System Diseases
 Inflammation Antibodies  >1 publications with ANXA13 and Inflammation
Organs/Tissues associated with ANXA13 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >1 publications with ANXA13 and Stomach
 Intestine Antibodies  >1 publications with ANXA13 and Intestine
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