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ADH elisa kit :: Human Antidiuretic Hormone (ADH) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS264445
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Antidiuretic Hormone (ADH), ELISA Kit

 Also Known As   

Human Antidiuretic Hormone (ADH) ELISA Kit

 Product Gene Name   

ADH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    304800
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 Species Reactivity    Human
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADH concentration
 Assay Type    Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADH purchase    MBS264445 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Antidiuretic Hormone (ADH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing ADH, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS264445 kit is based on ADH antibody-ADH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADH concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADH elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ADH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ADH elisa kit    ADH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS264445. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADH. It may not necessarily be applicable to this product.
 NCBI GI #    532760
 NCBI GeneID    554
 NCBI Accession #    AAC09005.1 [Other Products]
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 UniProt Secondary Accession #    O43192; Q3MJD3; Q9UCV9; C5HF20 [Other Products]
 UniProt Related Accession #    P30518 [Other Products]
 Molecular Weight    33,743 Da
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 NCBI Official Full Name    arginine vasopressin receptor type II, V2 antidiuretic hormone receptor
 NCBI Official Synonym Full Names    arginine vasopressin receptor 2
 NCBI Official Symbol    AVPR2 [Similar Products]
 NCBI Official Synonym Symbols   
DI1; DIR; NDI; V2R; ADHR; DIR3
[Similar Products]
 NCBI Protein Information    vasopressin V2 receptor; AVPR V2; antidiuretic hormone receptor; renal-type arginine vasopressin receptor
 UniProt Protein Name    Vasopressin V2 receptor
 UniProt Synonym Protein Names   
AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
 Protein Family    Alcohol dehydrogenase
 UniProt Gene Name    AVPR2 [Similar Products]
 UniProt Synonym Gene Names    ADHR; DIR; DIR3; V2R; V2R [Similar Products]
 UniProt Entry Name    V2R_HUMAN
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 NCBI Summary for ADH    This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADH    AVPR2: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption. Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD). This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI); also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: Golgi apparatus; endoplasmic reticulum; integral to plasma membrane; integral to membrane; plasma membrane; endosome

Molecular Function: protein binding; peptide binding; vasopressin receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; water transport; adenylate cyclase activation; positive regulation of systemic arterial blood pressure; excretion; cellular response to hormone stimulus; G-protein signaling, coupled to cAMP nucleotide second messenger; telencephalon development; regulation of systemic arterial blood pressure by vasopressin; hemostasis; positive regulation of protein ubiquitination; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of vasoconstriction; interferon-gamma production; renal water homeostasis; transmembrane transport

Disease: Nephrogenic Syndrome Of Inappropriate Antidiuresis; Diabetes Insipidus, Nephrogenic, X-linked
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 Research Articles on ADH    1. This review summarizes some of the unexpected roles of V2R signaling and suggests that vasopressin signaling itself may contribute crucially to loss of polarity and enhanced proliferation in cystic kidney epithelium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADH elisa kitPathways associated with ADH elisa kit
 Reference Product  PubMed Publications
 AVP elisa kit  >45 publications with ADH and AVP
 POMC elisa kit  >1 publications with ADH and POMC
 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
Diseases associated with ADH elisa kitOrgans/Tissues associated with ADH elisa kit
 Disease Name  Pubmed Publications
 Diabetes Insipidus, Nephrogenic Antibodies  >97 publications with ADH and Diabetes Insipidus, Nephrogenic
 Kidney Diseases Antibodies  >89 publications with ADH and Kidney Diseases
 Polyuria Antibodies  >26 publications with ADH and Polyuria
 Hyponatremia Antibodies  >14 publications with ADH and Hyponatremia
 Genetic Diseases, X-Linked Antibodies  >13 publications with ADH and Genetic Diseases, X-Linked
 Brain Diseases Antibodies  >12 publications with ADH and Brain Diseases
 Inappropriate ADH Syndrome Antibodies  >11 publications with ADH and Inappropriate ADH Syndrome
 Nephrogenic Syndrome of Inappropriate Antidiuresis Antibodies  >9 publications with ADH and Nephrogenic Syndrome of Inappropriate Antidiuresis
 Disease Models, Animal Antibodies  >4 publications with ADH and Disease Models, Animal
 Hypertension Antibodies  >2 publications with ADH and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with ADH and Lung
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