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APOD elisa kit :: Human Apolipoprotein D (APOD) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020944 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein D (APOD), ELISA Kit

★Popular Item★
 Also Known As   

Apolipoprotein D (APOD) ELISA Kit

 Product Synonym Names    Apo-D
 Product Gene Name   

APOD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    107740
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Apolipoprotein D (APOD).
No significant cross-reactivity or interference between Apolipoprotein D (APOD) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 0.67ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Apolipoprotein D (APOD) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Apolipoprotein D (APOD) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APOD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APOD purchase    MBS2020944 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein D (APOD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing APOD, with an estimated sensitivity of < 0.67ng/mL. The ELISA analytical biochemical technique of the MBS2020944 kit is based on APOD antibody-APOD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APOD elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein D (APOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein D (APOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein D (APOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein D (APOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for APOD elisa kit    Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism
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 Typical Testing Data/Standard Curve (for reference only) of APOD elisa kit    APOD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020944. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APOD. It may not necessarily be applicable to this product.
 NCBI GI #    178841
 NCBI GeneID    347
 NCBI Accession #    AAB59517.1 [Other Products]
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 UniProt Secondary Accession #    Q6IBG6; B2R579; D3DNW6 [Other Products]
 UniProt Related Accession #    P05090 [Other Products]
 Molecular Weight    21,276 Da
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 NCBI Official Full Name    apolipoprotein D
 NCBI Official Synonym Full Names    apolipoprotein D
 NCBI Official Symbol    APOD [Similar Products]
 NCBI Protein Information    apolipoprotein D
 UniProt Protein Name    Apolipoprotein D
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOD [Similar Products]
 UniProt Synonym Gene Names    Apo-D; ApoD [Similar Products]
 UniProt Entry Name    APOD_HUMAN
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 NCBI Summary for APOD    This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]
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 UniProt Comments for APOD    APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; dendrite; extracellular region

Molecular Function: lipid transporter activity; protein binding; cholesterol binding

Biological Process: response to drug; response to reactive oxygen species; tissue regeneration; negative regulation of smooth muscle cell proliferation; negative regulation of focal adhesion formation; glucose metabolic process; negative regulation of protein import into nucleus; lipid metabolic process; angiogenesis; brain development; response to axon injury; lipid transport; axon regeneration in the peripheral nervous system; aging
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 Research Articles on APOD    1. ApoE is located in the nucleus and on the ApoD promoter in human hepatic and glioblastoma cells lines.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APOD elisa kitPathways associated with APOD elisa kit
 Reference Product  PubMed Publications
 LEPR elisa kit  >2 publications with APOD and LEPR
 Products by Pathway  Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Fatty Acids Pathway antibodies  Transport Of Fatty Acids Pathway Diagram
 Transport Of Vitamins, Nucleosides, And Related Molecules Pathway antibodies  Transport Of Vitamins, Nucleosides, And Related Molecules Pathway Diagram
Diseases associated with APOD elisa kitOrgans/Tissues associated with APOD elisa kit
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >191 publications with APOD and Skin Diseases
 Breast Neoplasms Antibodies  >155 publications with APOD and Breast Neoplasms
 Carcinoma Antibodies  >124 publications with APOD and Carcinoma
 Adenocarcinoma Antibodies  >67 publications with APOD and Adenocarcinoma
 Nervous System Diseases Antibodies  >50 publications with APOD and Nervous System Diseases
 Skin Neoplasms Antibodies  >44 publications with APOD and Skin Neoplasms
 Brain Diseases Antibodies  >39 publications with APOD and Brain Diseases
 Neurodegenerative Diseases Antibodies  >27 publications with APOD and Neurodegenerative Diseases
 Cardiovascular Diseases Antibodies  >15 publications with APOD and Cardiovascular Diseases
 Metaplasia Antibodies  >15 publications with APOD and Metaplasia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >78 publications with APOD and Brain
 Skin Antibodies  >70 publications with APOD and Skin
 Nerve Antibodies  >29 publications with APOD and Nerve
 Liver Antibodies  >27 publications with APOD and Liver
 Mammary Gland Antibodies  >23 publications with APOD and Mammary Gland
 Muscle Antibodies  >21 publications with APOD and Muscle
 Prostate Antibodies  >18 publications with APOD and Prostate
 Vascular Antibodies  >17 publications with APOD and Vascular
 Lung Antibodies  >16 publications with APOD and Lung
 Lymph Node Antibodies  >14 publications with APOD and Lymph Node
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