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ALOX15B elisa kit :: Human Arachidonate 15-lipoxygenase B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760926
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Arachidonate 15-lipoxygenase B (ALOX15B), ELISA Kit

★Popular Item★
 Also Known As   

Human Arachidonate 15-lipoxygenase B ELISA Kit

 Product Synonym Names    ALOX15B (Arachidonate 15-lipoxygenase B)/15-LOX-B/15-LOX-2/Linoleate 13-lipoxygenase 15-Lob/15-lipoxygenase 2/Arachidonate 15-lipoxygenase type II
 Product Gene Name   

ALOX15B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760926 COA    COA PDF
 Species Reactivity    Human
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.312-20ng/ml
 Sensitivity    0.188ng/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALOX15B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALOX15B purchase    MBS760926 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/ml in biological research samples containing ALOX15B, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS760926 kit is based on ALOX15B antibody-ALOX15B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALOX15B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALOX15B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALOX15B elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ALOX15B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ALOX15B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ALOX15B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ALOX15B can be calculated.
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 Typical Testing Data of ALOX15B elisa kit    ALOX15B elisa kit Typical Testing Data image
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 Typical Standard Curve of ALOX15B elisa kit    ALOX15B elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS760926. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALOX15B. It may not necessarily be applicable to this product.
 NCBI GI #    23463277
 NCBI GeneID    266604
 NCBI Accession #    NP_695213.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_153301.2 [Other Products]
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 UniProt Related Accession #    Q8K4F2 [Other Products]
 Molecular Weight    76,145 Da
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 NCBI Official Full Name    arachidonate 15-lipoxygenase B
 NCBI Official Synonym Full Names    arachidonate 15-lipoxygenase, type B
 NCBI Official Symbol    Alox15b [Similar Products]
 NCBI Protein Information    arachidonate 15-lipoxygenase B
 UniProt Protein Name    Arachidonate 15-lipoxygenase B
 UniProt Synonym Protein Names   
15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)
 Protein Family    Arachidonate 15-lipoxygenase
 UniProt Gene Name    Alox15b [Similar Products]
 UniProt Synonym Gene Names    15-LOX-B; 15-LOX-2 [Similar Products]
 UniProt Entry Name    LX15B_RAT
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 NCBI Summary for ALOX15B    human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]
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 UniProt Comments for ALOX15B    ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; nucleus; plasma membrane

Molecular Function: arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipid binding; lipoxygenase activity

Biological Process: arachidonic acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of growth; positive regulation of keratinocyte differentiation
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 Research Articles on ALOX15B    1. Positive feedback-loop of TERT and 15-lipoxygenase-2 promotes pulmonary hypertension.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALOX15B elisa kitPathways associated with ALOX15B elisa kit
 Reference Product  PubMed Publications
 Alox15 elisa kit  >56 publications with ALOX15B and Alox15
 Ptgs2 elisa kit  >2 publications with ALOX15B and Ptgs2
 Ptgs1 elisa kit  >2 publications with ALOX15B and Ptgs1
 Alox12e elisa kit  >1 publications with ALOX15B and Alox12e
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Serotonergic Synapse Pathway antibodies  Serotonergic Synapse Pathway Diagram
 Synthesis Of 15-eicosatetraenoic Acid Derivatives Pathway antibodies  Synthesis Of 15-eicosatetraenoic Acid Derivatives Pathway Diagram
Diseases associated with ALOX15B elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >19 publications with ALOX15B and Inflammation
 Cardiovascular Diseases Antibodies  >8 publications with ALOX15B and Cardiovascular Diseases
 Atherosclerosis Antibodies  >6 publications with ALOX15B and Atherosclerosis
 Adenocarcinoma Antibodies  >4 publications with ALOX15B and Adenocarcinoma
 Lung Neoplasms Antibodies  >3 publications with ALOX15B and Lung Neoplasms
 Nervous System Diseases Antibodies  >3 publications with ALOX15B and Nervous System Diseases
 Kidney Diseases Antibodies  >2 publications with ALOX15B and Kidney Diseases
 Skin Neoplasms Antibodies  >2 publications with ALOX15B and Skin Neoplasms
 Lung Diseases Antibodies  >2 publications with ALOX15B and Lung Diseases
 Prostatic Hyperplasia Antibodies  >1 publications with ALOX15B and Prostatic Hyperplasia
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