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ARSA elisa kit :: Human Arylsulfatase A (ARSA) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2000254 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Arylsulfatase A (ARSA), ELISA Kit

★Popular Item★
 Also Known As   

Arylsulfatase A (ARSA) ELISA Kit

 Product Synonym Names    MLD; ASA; Metachromatic Leucodystrophy; Cerebroside-sulfatase
 Product Gene Name   

ARSA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    250100
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Arylsulfatase A (ARSA).
No significant cross-reactivity or interference between Arylsulfatase A (ARSA) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.056ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Arylsulfatase A (ARSA) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Arylsulfatase A (ARSA) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ARSA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ARSA purchase    MBS2000254 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arylsulfatase A (ARSA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ARSA, with an estimated sensitivity of < 0.056ng/mL. The ELISA analytical biochemical technique of the MBS2000254 kit is based on ARSA antibody-ARSA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARSA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARSA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ARSA elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase A (ARSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase A (ARSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase A (ARSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylsulfatase A (ARSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ARSA elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of ARSA elisa kit    ARSA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2000254. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ARSA. It may not necessarily be applicable to this product.
 NCBI GI #    1037140
 NCBI GeneID    410
 NCBI Accession #    AAB35013.1 [Other Products]
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 UniProt Secondary Accession #    Q6ICI5; Q96CJ0; B2RCA6; B7XD04; F8WCC8 [Other Products]
 UniProt Related Accession #    P15289 [Other Products]
 Molecular Weight    44,881 Da
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 NCBI Official Full Name    arylsulfatase A, partial
 NCBI Official Synonym Full Names    arylsulfatase A
 NCBI Official Symbol    ARSA [Similar Products]
 NCBI Official Synonym Symbols   
MLD
[Similar Products]
 NCBI Protein Information    arylsulfatase A
 UniProt Protein Name    Arylsulfatase A
 UniProt Synonym Protein Names   
Cerebroside-sulfatase
 Protein Family    Arylsulfatase
 UniProt Gene Name    ARSA [Similar Products]
 UniProt Synonym Gene Names    ASA [Similar Products]
 UniProt Entry Name    ARSA_HUMAN
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 NCBI Summary for ARSA    The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]
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 UniProt Comments for ARSA    ARSA: Hydrolyzes cerebroside sulfate. Defects in ARSA are a cause of leukodystrophy metachromatic (MLD). MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late- infantile, juvenile and adult. Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD). A clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Arylsulfatase A activity is impaired in multiple sulfatase deficiency due to mutations in SUMF1. SUMF1 mutations result in defective post-translational modification of ARSA at residue Cys- 69 that is not converted to 3-oxoalanine. Belongs to the sulfatase family.

Protein type: Hydrolase; Lipid Metabolism - sphingolipid; EC 3.1.6.8

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: lysosomal lumen; extracellular space; extrinsic to external side of plasma membrane; endoplasmic reticulum lumen; lysosome; acrosome; integral to membrane; endosome

Molecular Function: arylsulfatase activity; sulfuric ester hydrolase activity; calcium ion binding; cerebroside-sulfatase activity

Biological Process: cellular protein metabolic process; central nervous system development; sphingolipid metabolic process; response to ethanol; binding of sperm to zona pellucida; response to estrogen stimulus; response to methylmercury; autophagy; glycosphingolipid metabolic process; post-translational protein modification; response to nutrient; response to pH

Disease: Metachromatic Leukodystrophy
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 Research Articles on ARSA    1. Data indicate a significant correlation between the mutation of c.622delC(p.His208Metfs*46) in the arylsulfatase A (ARSA) gene and the phenotype OF metachromatic leukodystrophy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ARSA elisa kitPathways associated with ARSA elisa kit
 Reference Product  PubMed Publications
 ARSB elisa kit  >66 publications with ARSA and ARSB
 ARSD elisa kit  >24 publications with ARSA and ARSD
 ARSH elisa kit  >4 publications with ARSA and ARSH
 STS elisa kit  >3 publications with ARSA and STS
 GALC elisa kit  >3 publications with ARSA and GALC
 SUMF1 elisa kit  >3 publications with ARSA and SUMF1
 ARSI elisa kit  >1 publications with ARSA and ARSI
 SUMF2 elisa kit  >1 publications with ARSA and SUMF2
 Products by Pathway  Pathway Diagram
 Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies  Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
Diseases associated with ARSA elisa kitOrgans/Tissues associated with ARSA elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >118 publications with ARSA and Nervous System Diseases
 Leukodystrophy, Metachromatic Antibodies  >101 publications with ARSA and Leukodystrophy, Metachromatic
 Neurobehavioral Manifestations Antibodies  >24 publications with ARSA and Neurobehavioral Manifestations
 Peripheral Nervous System Diseases Antibodies  >6 publications with ARSA and Peripheral Nervous System Diseases
 Leukemia Antibodies  >4 publications with ARSA and Leukemia
 Liver Diseases Antibodies  >4 publications with ARSA and Liver Diseases
 Poisoning Antibodies  >3 publications with ARSA and Poisoning
 Breast Neoplasms Antibodies  >2 publications with ARSA and Breast Neoplasms
 Weight Loss Antibodies  >2 publications with ARSA and Weight Loss
 Anemia Antibodies  >2 publications with ARSA and Anemia
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >71 publications with ARSA and Vascular
 Blood Antibodies  >60 publications with ARSA and Blood
 Brain Antibodies  >34 publications with ARSA and Brain
 Heart Antibodies  >26 publications with ARSA and Heart
 Esophagus Antibodies  >17 publications with ARSA and Esophagus
 Nerve Antibodies  >13 publications with ARSA and Nerve
 Liver Antibodies  >13 publications with ARSA and Liver
 Muscle Antibodies  >11 publications with ARSA and Muscle
 Kidney Antibodies  >9 publications with ARSA and Kidney
 Trachea Antibodies  >8 publications with ARSA and Trachea
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