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ASPN elisa kit :: Human Asporin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS043530 ASPN elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Asporin (ASPN), ELISA Kit

★Popular Item★
 Also Known As   

Human Asporin ELISA Kit

 Product Gene Name   

ASPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 9; NC_000009.11 (95218487..95244844, complement). Location: 9q22
 OMIM    603932
 3D Structure    ModBase 3D Structure for Q9BXN1
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Body fluids, tissue homogenates, secretions or feces samples
 Assay Type    Quantitative Sandwich
 Detection Range    0.625 ng/ml - 20 ng/ml
 Sensitivity    0.1 ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ASPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ASPN purchase    MBS043530 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Asporin (ASPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml - 20 ng/ml in biological research samples containing ASPN, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS043530 kit is based on ASPN antibody-ASPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ASPN elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ASPN (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS043530. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ASPN. It may not necessarily be applicable to this product.
 NCBI GI #    301069324
 NCBI GeneID    54829
 NCBI Accession #    NP_001180264.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001193335.1 [Other Products]
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 UniProt Primary Accession #    Q9BXN1 [Other Products]
 UniProt Secondary Accession #    Q5TBF3; Q96K79; Q96LD0; Q9NXP3 [Other Products]
 UniProt Related Accession #    Q9BXN1 [Other Products]
 Molecular Weight    43,417 Da
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 NCBI Official Full Name    asporin isoform 2 preproprotein
 NCBI Official Synonym Full Names    asporin
 NCBI Official Symbol    ASPN [Similar Products]
 NCBI Official Synonym Symbols   
OS3; PLAP1; PLAP-1; SLRR1C
[Similar Products]
 NCBI Protein Information    asporin; asporin proteoglycan; asporin (LRR class 1); small leucine-rich protein 1C; periodontal ligament associated protein 1
 UniProt Protein Name    Asporin
 UniProt Synonym Protein Names   
Periodontal ligament-associated protein 1
 Protein Family    Asporin
 UniProt Gene Name    ASPN [Similar Products]
 UniProt Synonym Gene Names    PLAP1; SLRR1C; PLAP-1 [Similar Products]
 UniProt Entry Name    ASPN_HUMAN
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 NCBI Summary for ASPN    This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]
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 UniProt Comments for ASPN    ASPN: Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF- beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3); also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis. Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494). Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: collagen binding; calcium ion binding

Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway; bone mineralization

Disease: Intervertebral Disc Disease; Osteoarthritis Susceptibility 3
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 Research Articles on ASPN    1. mir-101 and mir-21 target PLAP-1 to regulate its expression during osteogenic differentiation of PDLCs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ASPN elisa kitPathways associated with ASPN elisa kit
 Reference Product  PubMed Publications
 FRZB elisa kit  >6 publications with ASPN and FRZB
 COL11A1 elisa kit  >3 publications with ASPN and COL11A1
 BMP2 elisa kit  >2 publications with ASPN and BMP2
 COL3A1 elisa kit  >2 publications with ASPN and COL3A1
 OMD elisa kit  >1 publications with ASPN and OMD
 Products by Pathway  Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with ASPN elisa kitOrgans/Tissues associated with ASPN elisa kit
 Disease Name  Pubmed Publications
 Osteoarthritis Antibodies  >45 publications with ASPN and Osteoarthritis
 Arthritis Antibodies  >40 publications with ASPN and Arthritis
 Immune System Diseases Antibodies  >4 publications with ASPN and Immune System Diseases
 Breast Neoplasms Antibodies  >3 publications with ASPN and Breast Neoplasms
 Brain Diseases Antibodies  >3 publications with ASPN and Brain Diseases
 Congenital Abnormalities Antibodies  >3 publications with ASPN and Congenital Abnormalities
 Fibrosis Antibodies  >3 publications with ASPN and Fibrosis
 Adenocarcinoma Antibodies  >2 publications with ASPN and Adenocarcinoma
 Hemorrhage Antibodies  >2 publications with ASPN and Hemorrhage
 Drug Toxicity Antibodies  >1 publications with ASPN and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >29 publications with ASPN and Bone
 Mouth Antibodies  >6 publications with ASPN and Mouth
 Muscle Antibodies  >6 publications with ASPN and Muscle
 Brain Antibodies  >5 publications with ASPN and Brain
 Embryonic Tissue Antibodies  >5 publications with ASPN and Embryonic Tissue
 Heart Antibodies  >4 publications with ASPN and Heart
 Connective Tissue Antibodies  >4 publications with ASPN and Connective Tissue
 Skin Antibodies  >3 publications with ASPN and Skin
 Liver Antibodies  >3 publications with ASPN and Liver
 Vascular Antibodies  >3 publications with ASPN and Vascular
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