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ATG9A elisa kit :: Human Autophagy related protein 9A (ATG9A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7221417
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Autophagy related protein 9A (ATG9A), ELISA Kit

★Popular Item★
 Also Known As   

Human Autophagy related protein 9A (ATG9A) ELISA Kit

 Product Gene Name   

ATG9A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612204
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ATG9A. No significant cross-reactivity or interference between ATG9A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG9A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ATG9A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ATG9A purchase    MBS7221417 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Autophagy related protein 9A (ATG9A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing ATG9A, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7221417 kit is based on ATG9A antibody-ATG9A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATG9A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATG9A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ATG9A elisa kit

   Intended Uses: This ATG9A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG9A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ATG9A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG9A antibody and an ATG9A-HRP conjugate. The assay sample and buffer are incubated together with ATG9A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG9A concentration since ATG9A from samples and ATG9A-HRP conjugate compete for the anti-ATG9A antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG9A from the sample, fewer sites are left to bind ATG9A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG9A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ATG9A elisa kit    Cardiovascular
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 Typical Standard Curve/Testing Data of ATG9A elisa kit    ATG9A elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7221417. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ATG9A. It may not necessarily be applicable to this product.
 NCBI GI #    116089287
 NCBI GeneID    79065
 NCBI Accession #    NP_076990.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_024085.4 [Other Products]
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 UniProt Secondary Accession #    Q3ZAQ6; Q6P0N7; Q7Z317; Q7Z320; Q8NDK6; Q8WU65; Q9BVL5; Q9H6L1; Q9HAG7 [Other Products]
 UniProt Related Accession #    Q7Z3C6 [Other Products]
 Molecular Weight    60,694 Da
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 NCBI Official Full Name    autophagy-related protein 9A
 NCBI Official Synonym Full Names    autophagy related 9A
 NCBI Official Symbol    ATG9A [Similar Products]
 NCBI Official Synonym Symbols   
mATG9; APG9L1; MGD3208
[Similar Products]
 NCBI Protein Information    autophagy-related protein 9A; APG9-like 1; APG9 autophagy 9-like 1; autophagy 9-like 1 protein; ATG9 autophagy related 9 homolog A
 UniProt Protein Name    Autophagy-related protein 9A
 UniProt Synonym Protein Names   
APG9-like 1; mATG9
 Protein Family    Autophagy-related protein
 UniProt Gene Name    ATG9A [Similar Products]
 UniProt Synonym Gene Names    APG9L1 [Similar Products]
 UniProt Entry Name    ATG9A_HUMAN
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 UniProt Comments for ATG9A    ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle; Membrane protein, multi-pass; Autophagy

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: endoplasmic reticulum membrane; recycling endosome; membrane; late endosome membrane; late endosome; integral to membrane; autophagic vacuole; trans-Golgi network; cytoplasmic vesicle; endosome

Molecular Function: protein binding

Biological Process: protein transport; protein localization in Golgi apparatus; mitochondrion degradation; innate immune response; autophagic vacuole formation
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 Research Articles on ATG9A    1. miR-29b mRNA, MAPK10 protein expression, and ATG9A protein expression are closely related to chemosensitivity of ovarian carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ATG9A elisa kitOrgans/Tissues associated with ATG9A elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >3 publications with ATG9A and Death
 Immune System Diseases Antibodies  >1 publications with ATG9A and Immune System Diseases
 Ovarian Diseases Antibodies  >1 publications with ATG9A and Ovarian Diseases
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2 publications with ATG9A and Heart
 Kidney Antibodies  >2 publications with ATG9A and Kidney
 Brain Antibodies  >2 publications with ATG9A and Brain
 Vascular Antibodies  >1 publications with ATG9A and Vascular
 Nerve Antibodies  >1 publications with ATG9A and Nerve
 Intestine Antibodies  >1 publications with ATG9A and Intestine
 Mouth Antibodies  >1 publications with ATG9A and Mouth
 Lung Antibodies  >1 publications with ATG9A and Lung
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