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| Product Name
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BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L), ELISA Kit ★Popular Item★
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| Also Known As
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Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BNIP3L ELISA Kit
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| Product Synonym Names
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Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) ELISA kit; BNIP3a; NIX; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19kD-interacting protein 3-like; adenovirus E1B19k-binding protein B5; BCL2/adenovirus E1B 19kDa interacting protein 3-like
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS903923 Testing Data
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| OMIM
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605368
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| 3D Structure
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ModBase 3D Structure for O60238
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of human BNIP3L. No significant cross-reactivity or interference between human BNIP3L and analogues was observed.
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| Samples
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Serum, plasma, cell culture supernates, Cell lysates
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| Assay Type
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Sandwich
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| Detection Range
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0.312 ng/ml -20 ng/ml.
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| Sensitivity
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The minimum detectable dose of human BNIP3L is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of BNIP3L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for BNIP3L purchase
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MBS903923 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing BNIP3L, with an estimated sensitivity of The minimum detectable dose of human BNIP3L is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS903923 kit is based on BNIP3L antibody-BNIP3L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BNIP3L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BNIP3L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BNIP3L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BNIP3L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BNIP3L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BNIP3L bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| Typical Testing Data/Standard Curve (for reference only) of BNIP3L elisa kit
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| Sample Manual Insert of MBS903923. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for BNIP3L. It may not necessarily be applicable to this product. |
| NCBI GI #
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4757860
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| NCBI GeneID
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665
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| NCBI Accession #
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NP_004322.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_004331.2
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[Other Products]
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| UniProt Primary Accession #
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O60238
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[Other Products]
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| UniProt Secondary Accession #
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Q5JW63; Q8NF87
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[Other Products]
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| UniProt Related Accession #
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O60238
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[Other Products]
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| Molecular Weight
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23,930 Da
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| NCBI Official Full Name
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BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
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| NCBI Official Synonym Full Names
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BCL2/adenovirus E1B 19kDa interacting protein 3-like
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| NCBI Official Symbol
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BNIP3L
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[Similar Products]
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| NCBI Official Synonym Symbols
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NIX; BNIP3a
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[Similar Products]
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| NCBI Protein Information
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BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; NIP3L; NIP3-like protein X; adenovirus E1B19k-binding protein B5; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
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| UniProt Protein Name
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BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
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| UniProt Synonym Protein Names
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Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L
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| Protein Family
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BCL2/adenovirus E1B 19 kDa protein-interacting protein
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| UniProt Gene Name
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BNIP3L
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[Similar Products]
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| UniProt Synonym Gene Names
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BNIP3A; BNIP3H; NIX; NIP3L
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[Similar Products]
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| UniProt Entry Name
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BNI3L_HUMAN
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| NCBI Summary for BNIP3L
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This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein counteracts the apoptotic inducer BNIP3 and may play a role in tumor suppression. [provided by RefSeq, Mar 2011]
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| UniProt Comments for BNIP3L
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BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.
Protein type: Endoplasmic reticulum; Nuclear envelope; Apoptosis; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; integral to membrane; intrinsic to membrane; nuclear envelope
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding
Biological Process: viral reproduction; positive regulation of apoptosis; defense response to virus; negative regulation of apoptosis
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| Research Articles on BNIP3L
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1. Nix protein positively regulates NF-kappaB activation in gliomas
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with BNIP3L elisa kit | Pathways associated with BNIP3L elisa kit |
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| Diseases associated with BNIP3L elisa kit | Organs/Tissues associated with BNIP3L elisa kit |
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ELISA Kit 
