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BDNF elisa kit :: Human BDNF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355324
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $375 +1 FREE 8GB USB
 
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 Product Name   

BDNF, ELISA Kit

★Popular Item★
 Also Known As   

BDNF Human ELISA Kit

 Product Synonym Names    Human BDNF
 Product Gene Name   

BDNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Samples    human serum, plasma, body fluids, tissue lysates or cell culture supernatants
 Assay Type    Quantitative Sandwich
 Detection Range    31.2 pg/ml-2000 pg/ml
 Sensitivity    < 2 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BDNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BDNF purchase    MBS355324 is a ready-to-use microwell, strip-or-full plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BDNF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml-2000 pg/ml in biological research samples containing BDNF, with an estimated sensitivity of < 2 pg/ml. The ELISA analytical biochemical technique of the MBS355324 kit is based on BDNF antibody-BDNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BDNF elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BDNF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BDNF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BDNF can be calculated.

Background: Brain-derived neurotrophic factor, also known as BDNF, is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is a member of the "neurotrophin" family of growth factors, which are related to the NGF. It acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. Post mortem analysis has shown lowered levels of BDNF in the brain tissues of people with Alzheimer's disease.
 Applications Tested/Suitable for BDNF elisa kit   

Quantitative sELISA (EIA)

 Application Notes for BDNF elisa kit    For quantitative detection of BDNF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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  Typical Testing Data/Standard Curve (for reference only) of BDNF elisa kit    BDNF elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355324. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BDNF. It may not necessarily be applicable to this product.
 NCBI GI #    262342369
 NCBI Accession #    ACY54685.1 [Other Products]
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 NCBI Official Full Name    BDNF, partial
 Protein Family    BDNF/NT-3 growth factors receptor
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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