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Bid elisa kit :: Human BH3 Interacting Domain Death Agonist ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721310 Bid elisa kit
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BH3 Interacting Domain Death Agonist (Bid), ELISA Kit

★Popular Item★
 Also Known As   

Human BH3 Interacting Domain Death Agonist ELISA Kit

 Product Gene Name   

Bid elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601997
 3D Structure    ModBase 3D Structure for P55957
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BH3. No significant cross-reactivity or interference between BH3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BH3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Bid elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Bid purchase    MBS721310 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BH3 Interacting Domain Death Agonist (Bid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing Bid, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS721310 kit is based on Bid antibody-Bid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Bid antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Bid. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Bid elisa kit

   Intended Uses: This BH3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BH3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||BH3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BH3. Standards or samples are then added to the microtiter plate wells and BH3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BH3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BH3 are added to each well to "sandwich" the BH3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BH3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BH3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Bid elisa kit    Cell Biology
NCBI/Uniprot data below describe general gene information for Bid. It may not necessarily be applicable to this product.
 NCBI GI #    47678309
 NCBI GeneID    637
 NCBI Accession #    CAG30275.1 [Other Products]
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 UniProt Primary Accession #    P55957 [Other Products]
 UniProt Secondary Accession #    Q549M7; Q71T04; Q7Z4M9; Q8IY86 [Other Products]
 UniProt Related Accession #    P55957 [Other Products]
 Molecular Weight    11,263 Da
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 NCBI Official Full Name    BID
 NCBI Official Synonym Full Names    BH3 interacting domain death agonist
 NCBI Official Symbol    BID [Similar Products]
 NCBI Official Synonym Symbols   
FP497
[Similar Products]
 NCBI Protein Information    BH3-interacting domain death agonist; p22 BID; BID isoform Si6; BID isoform L(2); BID isoform ES(1b); desmocollin type 4; apoptic death agonist; Human BID coding sequence
 UniProt Protein Name    BH3-interacting domain death agonist
 UniProt Synonym Protein Names   
p22 BID; BID
 Protein Family    BH3-interacting domain death agonist
 UniProt Gene Name    BID [Similar Products]
 UniProt Synonym Gene Names    BID [Similar Products]
 UniProt Entry Name    BID_HUMAN
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 NCBI Summary for Bid    This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Bid    BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; neuron apoptosis; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization
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 Research Articles on Bid    1. Using gene reporter assays, we show that promoter variations in 11 intrinsic apoptosis genes, including ADPRT, APAF1, BCL2, BAD, BID, MCL1, BIRC4, BCL2L1, ENDOG, YWHAB, and YWHAQ, influence promoter activity in an allele-specific manner.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Bid elisa kitPathways associated with Bid elisa kit
 Reference Product  PubMed Publications
 CASP8 elisa kit  >1177 publications with Bid and CASP8
 BAX elisa kit  >1118 publications with Bid and BAX
 CASP3 elisa kit  >1026 publications with Bid and CASP3
 BCL2L1 elisa kit  >341 publications with Bid and BCL2L1
 FADD elisa kit  >206 publications with Bid and FADD
 BAK1 elisa kit  >117 publications with Bid and BAK1
 FASLG elisa kit  >60 publications with Bid and FASLG
 GZMB elisa kit  >58 publications with Bid and GZMB
 MCL1 elisa kit  >27 publications with Bid and MCL1
 Products by Pathway  Pathway Diagram
 Activation And Oligomerization Of BAK Protein Pathway antibodies  Activation And Oligomerization Of BAK Protein Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Activation, Translocation And Oligomerization Of BAX Pathway antibodies  Activation, Translocation And Oligomerization Of BAX Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Diseases associated with Bid elisa kitOrgans/Tissues associated with Bid elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >869 publications with Bid and Cardiovascular Diseases
 Lung Diseases Antibodies  >680 publications with Bid and Lung Diseases
 Necrosis Antibodies  >678 publications with Bid and Necrosis
 Gastrointestinal Diseases Antibodies  >635 publications with Bid and Gastrointestinal Diseases
 Nervous System Diseases Antibodies  >609 publications with Bid and Nervous System Diseases
 Pain Antibodies  >463 publications with Bid and Pain
 Heart Diseases Antibodies  >430 publications with Bid and Heart Diseases
 Diarrhea Antibodies  >343 publications with Bid and Diarrhea
 Nausea Antibodies  >324 publications with Bid and Nausea
 Hypertension Antibodies  >315 publications with Bid and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1853 publications with Bid and Blood
 Liver Antibodies  >572 publications with Bid and Liver
 Heart Antibodies  >523 publications with Bid and Heart
 Lung Antibodies  >517 publications with Bid and Lung
 Brain Antibodies  >374 publications with Bid and Brain
 Vascular Antibodies  >323 publications with Bid and Vascular
 Kidney Antibodies  >279 publications with Bid and Kidney
 Skin Antibodies  >245 publications with Bid and Skin
 Bone Antibodies  >210 publications with Bid and Bone
 Stomach Antibodies  >180 publications with Bid and Stomach
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