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SLC4A1 elisa kit :: Human Band 3 anion transport protein (SLC4A1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7230564
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Band 3 anion transport protein (SLC4A1), ELISA Kit

★Popular Item★
 Also Known As   

Human Band 3 anion transport protein (SLC4A1) ELISA Kit

 Product Gene Name   

SLC4A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612653
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SLC4A1. No significant cross-reactivity or interference between SLC4A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC4A1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    25-500ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SLC4A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SLC4A1 purchase    MBS7230564 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Band 3 anion transport protein (SLC4A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500ng/mL in biological research samples containing SLC4A1, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS7230564 kit is based on SLC4A1 antibody-SLC4A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC4A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC4A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SLC4A1 elisa kit

   Intended Uses: This SLC4A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC4A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: SLC4A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLC4A1 antibody and an SLC4A1-HRP conjugate. The assay sample and buffer are incubated together with SLC4A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC4A1 concentration since SLC4A1 from samples and SLC4A1-HRP conjugate compete for the anti-SLC4A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC4A1 from the sample, fewer sites are left to bind SLC4A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC4A1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SLC4A1 elisa kit    Neurobiology
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 Typical Standard Curve/Testing Data of SLC4A1 elisa kit    SLC4A1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7230564. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SLC4A1. It may not necessarily be applicable to this product.
 NCBI GI #    4507021
 NCBI GeneID    6521
 NCBI Accession #    NP_000333.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000342.3 [Other Products]
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 UniProt Secondary Accession #    P78487; Q1ZZ45; Q4KKW9; Q4VB84; Q9UCY7; Q9UDJ1; G4V2I6 [Other Products]
 UniProt Related Accession #    P02730 [Other Products]
 Molecular Weight    101,792 Da
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 NCBI Official Full Name    band 3 anion transport protein
 NCBI Official Synonym Full Names    solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
 NCBI Official Symbol    SLC4A1 [Similar Products]
 NCBI Official Synonym Symbols   
DI; FR; SW; WD; WR; AE1; WD1; BND3; EPB3; CD233; EMPB3; RTA1A
[Similar Products]
 NCBI Protein Information    band 3 anion transport protein; Diego blood group; Swann blood group; anion exchanger 1; anion exchanger-1; Froese blood group; Wright blood group; Waldner blood group; anion exchange protein 1; erythroid anion exchange protein; erythrocyte membrane protein band 3; solute carrier family 4, anion exchanger, number 1; solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
 UniProt Protein Name    Band 3 anion transport protein
 UniProt Synonym Protein Names   
Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; CD_antigen: CD233
 Protein Family    Band 3 anion exchange protein
 UniProt Gene Name    SLC4A1 [Similar Products]
 UniProt Synonym Gene Names    AE1; DI; EPB3; AE 1; Anion exchanger 1 [Similar Products]
 UniProt Entry Name    B3AT_HUMAN
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 NCBI Summary for SLC4A1    The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SLC4A1    SLC4A1: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, autosomal dominant (AD-dRTA). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with hemolytic anemia (dRTA-HA). A disease characterized by the association of hemolytic anemia with distal renal tubular acidosis, the reduced ability to acidify urine resulting in variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with normal red cell morphology (dRTA-NRC). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Belongs to the anion exchanger (TC 2.A.31) family.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cortical cytoskeleton; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane; Z disc

Molecular Function: protein C-terminus binding; bicarbonate transmembrane transporter activity; protein binding; protein homodimerization activity; protein anchor; chloride transmembrane transporter activity; inorganic anion exchanger activity; anion:anion antiporter activity; anion transmembrane transporter activity; ankyrin binding; actin binding

Biological Process: bicarbonate transport; cellular ion homeostasis; regulation of intracellular pH; ion transport; chloride transport; transmembrane transport; anion transport

Disease: Blood Group--swann System; Blood Group--froese; Renal Tubular Acidosis, Distal, Autosomal Dominant; Blood Group--wright Antigen; Renal Tubular Acidosis, Distal, With Hemolytic Anemia; Spherocytosis, Type 4; Blood Group--diego System; Malaria, Susceptibility To; Blood Group--waldner Type
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 Research Articles on SLC4A1    1. In 12% of our thalassemia major patients, we found two bands in the agarose gel-electrophoresis of PCR products from band 3 gene with a difference of 65 +/- 10 bp, equivalent to a deletion of 20 to 25 amino acids in band 3 protein.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SLC4A1 elisa kitPathways associated with SLC4A1 elisa kit
 Reference Product  PubMed Publications
 RHAG elisa kit  >8 publications with SLC4A1 and RHAG
 ANK1 elisa kit  >7 publications with SLC4A1 and ANK1
 EPB42 elisa kit  >6 publications with SLC4A1 and EPB42
 EPB41 elisa kit  >4 publications with SLC4A1 and EPB41
 CA2 elisa kit  >3 publications with SLC4A1 and CA2
 SLC4A1AP elisa kit  >2 publications with SLC4A1 and SLC4A1AP
 SYK elisa kit  >2 publications with SLC4A1 and SYK
 Products by Pathway  Pathway Diagram
 Bicarbonate Transporters Pathway antibodies  Bicarbonate Transporters Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies  Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram
 Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies  Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 O2/CO2 Exchange In Erythrocytes Pathway antibodies  O2/CO2 Exchange In Erythrocytes Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
Diseases associated with SLC4A1 elisa kitOrgans/Tissues associated with SLC4A1 elisa kit
 Disease Name  Pubmed Publications
 Anemia Antibodies  >58 publications with SLC4A1 and Anemia
 Acidosis, Renal Tubular Antibodies  >58 publications with SLC4A1 and Acidosis, Renal Tubular
 Hematologic Diseases Antibodies  >53 publications with SLC4A1 and Hematologic Diseases
 Anemia, Hemolytic Antibodies  >50 publications with SLC4A1 and Anemia, Hemolytic
 Kidney Diseases Antibodies  >46 publications with SLC4A1 and Kidney Diseases
 Elliptocytosis, Hereditary Antibodies  >22 publications with SLC4A1 and Elliptocytosis, Hereditary
 Spherocytosis, Hereditary Antibodies  >19 publications with SLC4A1 and Spherocytosis, Hereditary
 Renal Tubular Acidosis, Distal, With Hemolytic Anemia Antibodies  >15 publications with SLC4A1 and Renal Tubular Acidosis, Distal, With Hemolytic Anemia
 Malaria Antibodies  >9 publications with SLC4A1 and Malaria
 Hemolysis Antibodies  >8 publications with SLC4A1 and Hemolysis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >99 publications with SLC4A1 and Blood
 Kidney Antibodies  >49 publications with SLC4A1 and Kidney
 Brain Antibodies  >4 publications with SLC4A1 and Brain
 Heart Antibodies  >4 publications with SLC4A1 and Heart
 Bone Antibodies  >3 publications with SLC4A1 and Bone
 Embryonic Tissue Antibodies  >2 publications with SLC4A1 and Embryonic Tissue
 Muscle Antibodies  >2 publications with SLC4A1 and Muscle
 Spleen Antibodies  >1 publications with SLC4A1 and Spleen
 Bone Marrow Antibodies  >1 publications with SLC4A1 and Bone Marrow
 Liver Antibodies  >1 publications with SLC4A1 and Liver
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