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HSPG2 elisa kit :: Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765938
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), ELISA Kit

 Also Known As   

Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA Kit

 Product Synonym Names    HSPG2/PLC/HSPG/PRCAN/SJA/SJS/SJS1/Perlecan/endorepellin (domain V region)/heparan sulfate proteoglycan 2/perlecan proteoglycan/PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)/
 Product Gene Name   

HSPG2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    142461
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 Species Reactivity    Human
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.625-40ng/ml
 Sensitivity    0.375ng/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPG2 purchase    MBS765938 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/ml in biological research samples containing HSPG2, with an estimated sensitivity of 0.375ng/ml. The ELISA analytical biochemical technique of the MBS765938 kit is based on HSPG2 antibody-HSPG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSPG2 elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HSPG2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HSPG2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HSPG2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman HSPG2can be calculated.
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 Typical Testing Data of HSPG2 elisa kit    HSPG2 elisa kit Typical Testing Data image
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 Typical Standard Curve of HSPG2 elisa kit    HSPG2 elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS765938. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPG2. It may not necessarily be applicable to this product.
 NCBI GI #    126012571
 NCBI GeneID    3339
 NCBI Accession #    NP_005520.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_005529.6 [Other Products]
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 UniProt Secondary Accession #    Q16287; Q5SZI3; Q9H3V5 [Other Products]
 UniProt Related Accession #    P98160 [Other Products]
 Molecular Weight    468,830 Da
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 NCBI Official Full Name    basement membrane-specific heparan sulfate proteoglycan core protein isoform b
 NCBI Official Synonym Full Names    heparan sulfate proteoglycan 2
 NCBI Official Symbol    HSPG2 [Similar Products]
 NCBI Official Synonym Symbols   
PLC; SJA; SJS; HSPG; SJS1; PRCAN
[Similar Products]
 NCBI Protein Information    basement membrane-specific heparan sulfate proteoglycan core protein
 UniProt Protein Name    Basement membrane-specific heparan sulfate proteoglycan core protein
 UniProt Synonym Protein Names   
Perlecan; PLC
 Protein Family    Basement membrane-specific heparan sulfate proteoglycan core protein
 UniProt Gene Name    HSPG2 [Similar Products]
 UniProt Synonym Gene Names    HSPG; PLC [Similar Products]
 UniProt Entry Name    PGBM_HUMAN
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 NCBI Summary for HSPG2    This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
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 UniProt Comments for HSPG2    HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.1-p34

Cellular Component: basal lamina; extracellular matrix; extracellular region; extracellular space; focal adhesion; Golgi lumen; lysosomal lumen; plasma membrane

Molecular Function: metal ion binding; protein binding; protein C-terminus binding

Biological Process: angiogenesis; brain development; carbohydrate metabolic process; cardiac muscle development; cellular protein metabolic process; chondrocyte differentiation; chondroitin sulfate metabolic process; embryonic skeletal morphogenesis; endochondral ossification; extracellular matrix disassembly; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; lipoprotein metabolic process; phototransduction, visible light; protein localization; retinoid metabolic process; vitamin metabolic process

Disease: Dyssegmental Dysplasia, Silverman-handmaker Type; Schwartz-jampel Syndrome, Type 1
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 Research Articles on HSPG2    1. Rare variants in the HSPG2 gene potentially contribute to the idiopathic scoliosis phenotype in a subset of patients with idiopathic scoliosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPG2 elisa kitPathways associated with HSPG2 elisa kit
 Reference Product  PubMed Publications
 APOE elisa kit  >7 publications with HSPG2 and APOE
 FGF2 elisa kit  >7 publications with HSPG2 and FGF2
 VEGFA elisa kit  >2 publications with HSPG2 and VEGFA
 EGFR elisa kit  >1 publications with HSPG2 and EGFR
 NID2 elisa kit  >1 publications with HSPG2 and NID2
 NID1 elisa kit  >1 publications with HSPG2 and NID1
 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Amyloid Fiber Formation Pathway antibodies  Amyloid Fiber Formation Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway antibodies  Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway Diagram
 Defective B3GAT3 Causes JDSSDHD Pathway antibodies  Defective B3GAT3 Causes JDSSDHD Pathway Diagram
 Defective B4GALT7 Causes EDS, Progeroid Type Pathway antibodies  Defective B4GALT7 Causes EDS, Progeroid Type Pathway Diagram
 Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway antibodies  Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway Diagram
 Defective EXT2 Causes Exostoses 2 Pathway antibodies  Defective EXT2 Causes Exostoses 2 Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
Diseases associated with HSPG2 elisa kitOrgans/Tissues associated with HSPG2 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >21 publications with HSPG2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >9 publications with HSPG2 and Disease Models, Animal
 Osteochondrodysplasias Antibodies  >9 publications with HSPG2 and Osteochondrodysplasias
 Kidney Diseases Antibodies  >7 publications with HSPG2 and Kidney Diseases
 Neurodegenerative Diseases Antibodies  >6 publications with HSPG2 and Neurodegenerative Diseases
 Movement Disorders Antibodies  >6 publications with HSPG2 and Movement Disorders
 Dyssegmental dysplasia Antibodies  >6 publications with HSPG2 and Dyssegmental dysplasia
 Alzheimer Disease Antibodies  >5 publications with HSPG2 and Alzheimer Disease
 Hypertrophy Antibodies  >3 publications with HSPG2 and Hypertrophy
 Inflammation Antibodies  >3 publications with HSPG2 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >18 publications with HSPG2 and Bone
 Blood Antibodies  >16 publications with HSPG2 and Blood
 Muscle Antibodies  >14 publications with HSPG2 and Muscle
 Vascular Antibodies  >14 publications with HSPG2 and Vascular
 Connective Tissue Antibodies  >10 publications with HSPG2 and Connective Tissue
 Brain Antibodies  >6 publications with HSPG2 and Brain
 Kidney Antibodies  >6 publications with HSPG2 and Kidney
 Embryonic Tissue Antibodies  >6 publications with HSPG2 and Embryonic Tissue
 Mouth Antibodies  >5 publications with HSPG2 and Mouth
 Ovary Antibodies  >5 publications with HSPG2 and Ovary
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