View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Beta-APP42 elisa kit :: Human Beta-amyloid protein 42 ELISA Kit Catalog # MBS760107 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Beta-amyloid protein 42 (Beta-APP42), ELISA Kit Popular Item Full Product Name Human Beta-amyloid protein 42 ELISA Kit Product Synonym Names Beta-APP42 (Beta-amyloid protein 42) Product Gene Name Beta-APP42 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS760107 QC Report QC Report PDF Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of Beta-APP42. No significant cross-reactivity or interference between Beta-APP42 and analogues was observed. Samples Serum, plasma, tissue homogenates and other biological fluids. Assay Type Sandwich Detection Range 15.6-1000pg/ml Sensitivity < 9.375pg/ml Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-APP42 were tested 20 times on one plate, respectively. Intra-Assay: CV<8% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-APP42 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10% Preparation and Storage Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Beta-APP42 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Beta-APP42 purchase MBS760107 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-amyloid protein 42 (Beta-APP42) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-APP42. The ELISA analytical biochemical technique of the MBS760107 kit is based on Beta-APP42 antibody-Beta-APP42 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-APP42 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Beta-APP42. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Beta-APP42 elisa kit Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Beta- APP42 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Beta- APP42 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Beta-APP42 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Beta- APP42 can be calculated. Typical Testing Data/Standard Curve (for reference only) of Beta-APP42 elisa kit TOP Sample Manual Insert of MBS760107. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP