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MGEA5 elisa kit :: Human Bifunctional protein NCOAT (MGEA5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7213506 MGEA5 elisa kit
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Bifunctional protein NCOAT (MGEA5), ELISA Kit

 Also Known As   

Human Bifunctional protein NCOAT (MGEA5) ELISA Kit

 Product Gene Name   

MGEA5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    604039
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MGEA5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MGEA5 purchase    MBS7213506 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bifunctional protein NCOAT (MGEA5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MGEA5. The ELISA analytical biochemical technique of the MBS7213506 kit is based on MGEA5 antibody-MGEA5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MGEA5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MGEA5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for MGEA5. It may not necessarily be applicable to this product.
 NCBI GI #    215490056
 NCBI GeneID    10724
 NCBI Accession #    NP_001135906.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001142434.1 [Other Products]
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 UniProt Secondary Accession #    O75166; Q86WV0; Q8IV98; Q9BVA5; Q9HAR0; B7WPB9; D3DR79; E9PGF9 [Other Products]
 UniProt Related Accession #    O60502 [Other Products]
 Molecular Weight    96,932 Da
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 NCBI Official Full Name    bifunctional protein NCOAT isoform b
 NCBI Official Synonym Full Names    meningioma expressed antigen 5 (hyaluronidase)
 NCBI Official Symbol    MGEA5 [Similar Products]
 NCBI Official Synonym Symbols   
OGA; MEA5; NCOAT
[Similar Products]
 NCBI Protein Information    bifunctional protein NCOAT; O-GlcNAcase; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
 UniProt Protein Name    Bifunctional protein NCOAT
 UniProt Synonym Protein Names   
Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferaseIncluding the following 2 domains:Protein O-GlcNAcase (EC:3.2.1.169)Alternative name(s):Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA
 Protein Family    Protein O-GlcNAcase
 UniProt Gene Name    MGEA5 [Similar Products]
 UniProt Synonym Gene Names    HEXC; KIAA0679; MEA5; OGA; HAT [Similar Products]
 UniProt Entry Name    NCOAT_HUMAN
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 NCBI Summary for MGEA5    The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for MGEA5    OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Acetyltransferase; Hydrolase; EC 3.2.1.169; EC 3.2.1.52

Chromosomal Location of Human Ortholog: 10q24.1-q24.3

Cellular Component: membrane; cytosol; nucleus

Molecular Function: beta-N-acetylglucosaminidase activity; histone acetyltransferase activity; hyalurononglucosaminidase activity

Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; glycoprotein catabolic process; histone acetylation; protein targeting to membrane; aging
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 Research Articles on MGEA5    1. Estrogen replacement therapy and plyometric training influence muscle OGT and OGA gene expression, which may be one of the mechanisms by which HRT and PT prevent aging-related loss of muscle mass.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MGEA5 elisa kitPathways associated with MGEA5 elisa kit
 Reference Product  PubMed Publications
 OGT elisa kit  >6 publications with MGEA5 and OGT
 Products by Pathway  Pathway Diagram
 Protein O-[N-acetyl]-glucosylation Pathway antibodies  Protein O-[N-acetyl]-glucosylation Pathway Diagram
Diseases associated with MGEA5 elisa kitOrgans/Tissues associated with MGEA5 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with MGEA5 and Nervous System Diseases
 Inflammation Antibodies  >4 publications with MGEA5 and Inflammation
 Kidney Diseases Antibodies  >3 publications with MGEA5 and Kidney Diseases
 Breast Neoplasms Antibodies  >2 publications with MGEA5 and Breast Neoplasms
 Necrosis Antibodies  >1 publications with MGEA5 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with MGEA5 and Blood
 Bladder Antibodies  >3 publications with MGEA5 and Bladder
 Kidney Antibodies  >3 publications with MGEA5 and Kidney
 Muscle Antibodies  >2 publications with MGEA5 and Muscle
 Lymph Node Antibodies  >2 publications with MGEA5 and Lymph Node
 Skin Antibodies  >2 publications with MGEA5 and Skin
 Embryonic Tissue Antibodies  >2 publications with MGEA5 and Embryonic Tissue
 Lymph Antibodies  >2 publications with MGEA5 and Lymph
 Vascular Antibodies  >2 publications with MGEA5 and Vascular
 Connective Tissue Antibodies  >1 publications with MGEA5 and Connective Tissue
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