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BLVRB elisa kit :: Human Biliverdin Reductase B (BLVRB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019416 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
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 Product Name   

Biliverdin Reductase B (BLVRB), ELISA Kit

★Popular Item★
 Also Known As   

Biliverdin Reductase B (BLVRB) ELISA Kit

 Product Synonym Names    FLR; SDR43U1; Flavin Reductase,NADPH; Short Chain Dehydrogenase/Reductase Family 43U,Member 1; Biliverdin-IX beta-reductase; Green heme-binding protein
 Product Gene Name   

BLVRB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600941
 3D Structure    ModBase 3D Structure for P30043
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase B (BLVRB).
No significant cross-reactivity or interference between Biliverdin Reductase B (BLVRB) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.124ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Biliverdin Reductase B (BLVRB) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Biliverdin Reductase B (BLVRB) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BLVRB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BLVRB purchase    MBS2019416 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biliverdin Reductase B (BLVRB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing BLVRB, with an estimated sensitivity of < 0.124ng/mL. The ELISA analytical biochemical technique of the MBS2019416 kit is based on BLVRB antibody-BLVRB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BLVRB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BLVRB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BLVRB elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase B (BLVRB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase B (BLVRB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase B (BLVRB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biliverdin Reductase B (BLVRB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for BLVRB elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of BLVRB elisa kit    BLVRB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019416. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BLVRB. It may not necessarily be applicable to this product.
 NCBI GI #    81674202
 NCBI GeneID    645
 NCBI Accession #    AAI09372.1 [Other Products]
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 UniProt Primary Accession #    P30043 [Other Products]
 UniProt Secondary Accession #    P32078; P53005; Q32LZ2; A6NKD8; B2R5C6 [Other Products]
 UniProt Related Accession #    P30043 [Other Products]
 Molecular Weight    22,119 Da
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 NCBI Official Full Name    Biliverdin reductase B (flavin reductase (NADPH))
 NCBI Official Synonym Full Names    biliverdin reductase B
 NCBI Official Symbol    BLVRB [Similar Products]
 NCBI Official Synonym Symbols   
FLR; BVRB; SDR43U1; HEL-S-10
[Similar Products]
 NCBI Protein Information    flavin reductase (NADPH)
 UniProt Protein Name    Flavin reductase (NADPH)
 UniProt Synonym Protein Names   
Biliverdin reductase B (EC:1.3.1.24); BVR-B; Biliverdin-IX beta-reductase; Green heme-binding protein; GHBP; NADPH-dependent diaphorase; NADPH-flavin reductase; FLR
 UniProt Gene Name    BLVRB [Similar Products]
 UniProt Synonym Gene Names    FLR; FR; BVR-B; GHBP; FLR [Similar Products]
 UniProt Entry Name    BLVRB_HUMAN
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 NCBI Summary for BLVRB    The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]
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 UniProt Comments for BLVRB    BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Protein type: EC 1.5.1.30; Oxidoreductase; EC 1.3.1.24; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: flavin reductase activity; biliverdin reductase activity

Biological Process: heme catabolic process; porphyrin metabolic process
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 Research Articles on BLVRB    1. quantum mechanics/molecular mechanics potential energy surfaces show that the lowest energy pathway proceeds with a positively charged pyrrole intermediate via two transition states
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with BLVRB elisa kitDiseases associated with BLVRB elisa kit
 Products by Pathway  Pathway Diagram
 Heme Degradation Pathway antibodies  Heme Degradation Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Porphyrins Pathway antibodies  Metabolism Of Porphyrins Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Heme Degradation Pathway antibodies  Heme Degradation Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with BLVRB and Carcinoma
 Nervous System Diseases Antibodies  >1 publications with BLVRB and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with BLVRB and Liver Diseases
 Carcinoma, Squamous Cell Antibodies  >1 publications with BLVRB and Carcinoma, Squamous Cell
 Inflammation Antibodies  >1 publications with BLVRB and Inflammation
 Fatty Liver Antibodies  >1 publications with BLVRB and Fatty Liver
 Esophageal Squamous Cell Carcinoma Antibodies  >1 publications with BLVRB and Esophageal Squamous Cell Carcinoma
Organs/Tissues associated with BLVRB elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with BLVRB and Blood
 Prostate Antibodies  >2 publications with BLVRB and Prostate
 Liver Antibodies  >2 publications with BLVRB and Liver
 Bone Marrow Antibodies  >1 publications with BLVRB and Bone Marrow
 Bone Antibodies  >1 publications with BLVRB and Bone
 Ovary Antibodies  >1 publications with BLVRB and Ovary
 Lung Antibodies  >1 publications with BLVRB and Lung
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