View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List BTD elisa kit :: Human Biotinidase (BTD) ELISA Kit Catalog # MBS167321 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Biotinidase (BTD), ELISA Kit Full Product Name Human Biotinidase (BTD) ELISA Kit Product Gene Name BTD elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Human Samples Serum, plasma, cell culture supernates, cell lysates, tissue homogenates Assay Type Quantitative Sandwich Detection Range 0.5ng/ml - 150ng/ml Sensitivity 0.23ng/ml Intra-Assay CV<8% Inter-Assay CV<10% Preparation and Storage Store at 2 to 8 degree C for 6 months. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of BTD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for BTD purchase MBS167321 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biotinidase (BTD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BTD. The ELISA analytical biochemical technique of the MBS167321 kit is based on BTD antibody-BTD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BTD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BTD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for BTD elisa kit Intended Uses: This sandwich kit is for the accurate quantitative detection of human Biotinidase (also known as BTD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human BTD antibody. BTD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human BTD Antibody is added and binds to BTD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BTD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human BTD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm. Typical Testing Data/Standard Curve (for reference only) of BTD elisa kit TOP Sample Manual Insert of MBS167321. NCBI/Uniprot data below describe general gene information for BTD. It may not necessarily be applicable to this product. NCBI GI # 468824 NCBI GeneID 686 NCBI Accession # AAC04318.1 [Other Products] UniProt Secondary Accession # Q96EM9; A6NHF2; B2R865; B4DFX1; B4DLJ9; B7Z7C9; F8W1Q3[Other Products] UniProt Related Accession # P43251[Other Products] Molecular Weight 61,510 Da TOP NCBI Official Full Name biotinidase NCBI Official Synonym Full Names biotinidase NCBI Official Symbol BTD [Similar Products] NCBI Protein Information biotinidase UniProt Protein Name Biotinidase Protein Family Biotinidase UniProt Gene Name BTD [Similar Products] UniProt Synonym Gene Names Biotinase [Similar Products] TOP NCBI Summary for BTD The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] TOP UniProt Comments for BTD Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.CautionIt is uncertain whether Met-1 or Met-21 is the initiator. Research Articles on BTD 1. Biotinidase deficiency is reviewed. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with BTD elisa kit Reference Product PubMed Publications HLCS elisa kit >2 publications with BTD and HLCS ACACA elisa kit >1 publications with BTD and ACACA MCCC2 elisa kit >1 publications with BTD and MCCC2 MCCC1 elisa kit >1 publications with BTD and MCCC1 Pathways associated with BTD elisa kit Products by Pathway Pathway Diagram Biotin Metabolism Pathway antibodies Biotin Metabolism Pathway Diagram Biotin Metabolism Pathway antibodies Biotin Metabolism Pathway Diagram Biotin Transport And Metabolism Pathway antibodies Biotin Transport And Metabolism Pathway Diagram Metabolic Pathways antibodies Metabolic Pathways Diagram Metabolism Pathway antibodies Metabolism Pathway Diagram Metabolism Of Vitamins And Cofactors Pathway antibodies Metabolism Of Vitamins And Cofactors Pathway Diagram Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram Vitamin Digestion And Absorption Pathway antibodies Vitamin Digestion And Absorption Pathway Diagram Vitamin Digestion And Absorption Pathway antibodies Vitamin Digestion And Absorption Pathway Diagram Diseases associated with BTD elisa kit Disease Name Pubmed Publications Biotinidase Deficiency Antibodies >25 publications with BTD and Biotinidase Deficiency Cardiovascular Diseases Antibodies >14 publications with BTD and Cardiovascular Diseases Nervous System Diseases Antibodies >11 publications with BTD and Nervous System Diseases Kidney Diseases Antibodies >6 publications with BTD and Kidney Diseases Heart Diseases Antibodies >6 publications with BTD and Heart Diseases Acute Kidney Injury Antibodies >4 publications with BTD and Acute Kidney Injury Liver Diseases Antibodies >3 publications with BTD and Liver Diseases Inflammation Antibodies >3 publications with BTD and Inflammation Disease Models, Animal Antibodies >2 publications with BTD and Disease Models, Animal Fetal Death Antibodies >2 publications with BTD and Fetal Death Organs/Tissues associated with BTD elisa kit Organ/Tissue Name Pubmed Publications Blood Antibodies >38 publications with BTD and Blood Brain Antibodies >16 publications with BTD and Brain Thyroid Antibodies >12 publications with BTD and Thyroid Heart Antibodies >10 publications with BTD and Heart Lung Antibodies >9 publications with BTD and Lung Muscle Antibodies >8 publications with BTD and Muscle Vascular Antibodies >8 publications with BTD and Vascular Liver Antibodies >8 publications with BTD and Liver Bone Antibodies >8 publications with BTD and Bone Kidney Antibodies >7 publications with BTD and Kidney TOP © 2006-24 MyBioSource.com. 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