Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow c Jun rightarrow Human c Jun  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ATF2 Antibody
 • Abeta1-42 ELISA Kit
 • Immunoglobulin E (IgE) ELISA Kit
 • Alpha Fetoprotein (AFP) Antibody
 • STAPHYLOCOCCUS AUREUS Antibody
 • ATPase, H+ transporting, lysosomal 56-58kDa, V1 subunit B1 (... ELISA Kit
 • KLHL3 cDNA Clone
 • RAB32 Antibody
 • Inactive tyrosine-protein kinase 7 (PTK7) (PTK7 CCK4) Recombinant Protein
 • Ig Antibody
 • SMAD3 Antibody
 • DYNLT1 Recombinant Protein
 • Neurotrypsin  (PRSS12) Recombinant Protein
 • complexin 3 (CPLX3) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

c Jun elisa kit :: Human C Jun ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725273
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C Jun, ELISA Kit

 Also Known As   

Human C Jun ELISA Kit

 Product Gene Name   

c Jun elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    1.0ng/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of c Jun elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for c Jun purchase    MBS725273 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C Jun, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing c Jun, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS725273 kit is based on c Jun antibody-c Jun antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect c Jun antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, c Jun. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for c Jun elisa kit

   Intended Uses: This C-JUN ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofC-JUN in the sample, thisC-JUN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusC-JUN concentration. The concentration ofC-JUN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This C-JUN enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forC-JUN. Standards or samples are then added to the microtiter plate wells andC-JUN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofC-JUN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forC-JUN are added to each well to "sandwich" theC-JUN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containC-JUN and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for c Jun elisa kit    Cardiovascular
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of c Jun elisa kit    c Jun elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS725273. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for c Jun. It may not necessarily be applicable to this product.
 NCBI GI #    1911701
 NCBI GeneID    396913
 NCBI Accession #    AAB50808.1 [Other Products]
Section Bar
 UniProt Related Accession #    P56432 [Other Products]
 Molecular Weight    35,629 Da
Table BarTOPTable Bar
 NCBI Official Full Name    c-Jun
 NCBI Official Symbol    C-JUN [Similar Products]
 NCBI Official Synonym Symbols   
Jun
[Similar Products]
 NCBI Protein Information    transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
 UniProt Protein Name    Transcription factor AP-1
 UniProt Synonym Protein Names   
Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog
 UniProt Gene Name    JUN [Similar Products]
 UniProt Synonym Gene Names    AP1 [Similar Products]
 UniProt Entry Name    JUN_PIG
Section Bar
 UniProt Comments for c Jun    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells.
Table BarTOPTable Bar
 Research Articles on c Jun    1. The effects of prostaglandin F2alpha administration on transcription factor AP-1 expression and the expression of downstream genes involved in luteolysis are reported.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with c Jun elisa kitPathways associated with c Jun elisa kit
 Reference Product  PubMed Publications
 FOS elisa kit  >6553 publications with c Jun and FOS
 JNK1 elisa kit  >1278 publications with c Jun and JNK1
 IL6 elisa kit  >188 publications with c Jun and IL6
 IL8 elisa kit  >89 publications with c Jun and IL8
 MAPK14 elisa kit  >39 publications with c Jun and MAPK14
 FOSL1 elisa kit  >14 publications with c Jun and FOSL1
 MAPK9 elisa kit  >11 publications with c Jun and MAPK9
 MAPK10 elisa kit  >4 publications with c Jun and MAPK10
 MAPK13 elisa kit  >2 publications with c Jun and MAPK13
 Products by Pathway  Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
Organs/Tissues associated with c Jun elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6954 publications with c Jun and Brain
 Embryonic Tissue Antibodies  >1978 publications with c Jun and Embryonic Tissue
 Intestine Antibodies  >1256 publications with c Jun and Intestine
 Ovary Antibodies  >812 publications with c Jun and Ovary
 Adipose Tissue Antibodies  >670 publications with c Jun and Adipose Tissue
 Thymus Antibodies  >453 publications with c Jun and Thymus
 Uterus Antibodies  >449 publications with c Jun and Uterus
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions