Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource rightarrow ELISA Kit rightarrow CRAF rightarrow Human CRAF  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
Our Best Sellers moreseparator
 • HSP90beta Antibody
 • Tumor Necrosis Factor alpha (TNFalpha) ELISA Kit
 • Fibrinogen gamma prime (gamma' 408-427 specific), also calle... Antibody
 • Legumain (LGMN) ELISA Kit
 • M-oxotoxin-Ot1a (M-OXTX-Ot1a) Recombinant Protein
 • RBM35A (ESRP1) Antibody
 • Leukotriene C4 (LTC4) ELISA Kit
 • Surfactant Associated Protein D (SPD) ELISA Kit
 • Neurocalcin Delta (NCALD) Recombinant Protein
 • CD55 Antibody
 • Epstein Barr IgA ELISA Kit
 • Phospho-Exiracellular Signal-Regulated Kinase (PERK) ELISA Kit
 • Double-strand break repair protein MRE11A (Mre11a) Recombinant Protein
 • Galectin-3 (LGALS3) Recombinant Protein
 downarrow more ...
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CRAF elisa kit :: Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS077112 CRAF elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF), ELISA Kit

★Popular Item★
 Also Known As   

Human C-Raf Proto Oncogene Serine/Threonine Protein Kinase ELISA Kit

 Product Gene Name   

CRAF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q99N57
 Species Reactivity    Human
Section Bar
 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
Section Bar
 Samples    Undiluted original Human body fluids, tissue homogenates, secretions or feces samples.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.25ng/ml-8ng/ml.
 Sensitivity    0.1ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CRAF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CRAF purchase    MBS077112 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25ng/ml-8ng/ml in biological research samples containing CRAF, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS077112 kit is based on CRAF antibody-CRAF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRAF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRAF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar

Related Product Information for CRAF elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CRAF (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS077112. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CRAF. It may not necessarily be applicable to this product.
 NCBI GI #    18150196
 NCBI GeneID    110157
 NCBI Accession #    BAB83675.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q99N57 [Other Products]
 UniProt Secondary Accession #    Q3UR68; Q58E75; Q91WH1; Q99N58; Q9QUU8 [Other Products]
 UniProt Related Accession #    Q99N57 [Other Products]
 Molecular Weight    72,917 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cRAF, partial
 NCBI Official Synonym Full Names    v-raf-leukemia viral oncogene 1
 NCBI Official Symbol    Raf1 [Similar Products]
 NCBI Official Synonym Symbols   
Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
[Similar Products]
 NCBI Protein Information    RAF proto-oncogene serine/threonine-protein kinase; cRaf; Craf1 transforming; proto-oncogene c-RAF; sarcoma 3611 oncogene; murine sarcoma 3611 oncogene 1; v-raf-1 leukemia viral oncogene 1
 UniProt Protein Name    RAF proto-oncogene serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Proto-oncogene c-RAF; cRaf; Raf-1
 UniProt Gene Name    Raf1 [Similar Products]
 UniProt Synonym Gene Names    Craf; cRaf [Similar Products]
 UniProt Entry Name    RAF1_MOUSE
Section Bar
 UniProt Comments for CRAF    RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC; Kinase, protein; TKL group; RAF family

Cellular Component: Golgi apparatus; membrane; mitochondrion; cytoplasm; plasma membrane; pseudopodium; nucleus; cytosol

Molecular Function: identical protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; Ras GTPase binding; MAP kinase kinase kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; receptor signaling protein activity; kinase activity; ATP binding

Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; MAPKKK cascade; intermediate filament cytoskeleton organization and biogenesis; signal transduction; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of cell proliferation; response to hypoxia; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; negative regulation of protein complex assembly; phosphorylation; negative regulation of apoptosis
Table BarTOPTable Bar
 Research Articles on CRAF    1. PHLPP is a negative regulator of RAF1, which reduces colorectal cancer cell motility and prevents tumor progression in mice.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with CRAF elisa kitDiseases associated with CRAF elisa kit
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >163 publications with CRAF and Neoplasms
 Cardiovascular Diseases Antibodies  >80 publications with CRAF and Cardiovascular Diseases
 Noonan Syndrome Antibodies  >70 publications with CRAF and Noonan Syndrome
 Heart Diseases Antibodies  >62 publications with CRAF and Heart Diseases
 Nervous System Diseases Antibodies  >32 publications with CRAF and Nervous System Diseases
 Lung Neoplasms Antibodies  >25 publications with CRAF and Lung Neoplasms
 Cardiomyopathy, Hypertrophic Antibodies  >24 publications with CRAF and Cardiomyopathy, Hypertrophic
 Cell Transformation, Neoplastic Antibodies  >23 publications with CRAF and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >23 publications with CRAF and Breast Neoplasms
 LEOPARD Syndrome Antibodies  >22 publications with CRAF and LEOPARD Syndrome
Organs/Tissues associated with CRAF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >53 publications with CRAF and Heart
 Lung Antibodies  >43 publications with CRAF and Lung
 Muscle Antibodies  >28 publications with CRAF and Muscle
 Brain Antibodies  >26 publications with CRAF and Brain
 Liver Antibodies  >23 publications with CRAF and Liver
 Skin Antibodies  >21 publications with CRAF and Skin
 Kidney Antibodies  >18 publications with CRAF and Kidney
 Prostate Antibodies  >11 publications with CRAF and Prostate
 Bone Antibodies  >10 publications with CRAF and Bone
 Ovary Antibodies  >7 publications with CRAF and Ovary
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions