Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ASPH CT rightarrow Human ASPH CT  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • HLA-DP--DQ--DR Antibody
 • 25 OH Vitamin D3 Antibody
 • SerpinA11  Antibody
 • RANkappaL ELISA Kit
 • Glucose 6 Phosphate (G6P) ELISA Kit
 • GST Recombinant Protein
 • NMP-22 ELISA Kit
 • plasma kallikrein (KLKB1) ELISA Kit
 • Paired Immunoglobulin-Like Type 2 Receptor-Ligand (Cd99) Antibody
 • PKIa ELISA Kit
 • TFDP2 cDNA Clone
 • SMN Antibody
 • IDH1 Antibody
 • Actin (ACTA1) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ASPH CT elisa kit :: Human C Terminal Aspartate beta Hydroxylase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS748846
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C Terminal Aspartate beta Hydroxylase (ASPH CT), ELISA Kit

 Also Known As   

Human C Terminal Aspartate beta Hydroxylase ELISA Kit

 Product Synonym Names    Human C Terminal Aspartate b Hydroxylase ELISA Kit
 Product Gene Name   

ASPH CT elisa kit

[Similar Products]
 Product Synonym Gene Name    ASPH CT [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600582
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of ASPH-CT. No significant cross-reactivity or interference between ASPH-CT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASPH-CT and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    250-5000 pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This ASPH-CT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASPH-CT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ASPH CT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ASPH CT purchase    MBS748846 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C Terminal Aspartate beta Hydroxylase (ASPH CT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250-5000 pg/mL in biological research samples containing ASPH CT, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS748846 kit is based on ASPH CT antibody-ASPH CT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASPH CT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASPH CT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ASPH CT elisa kit

   Principle of the assay: ASPH-CT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASPH-CT. Standards or samples are then added to the microtiter plate wells and ASPH-CT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASPH-CT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASPH-CT are added to each well to "sandwich" the ASPH-CT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASPH-CT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASPH-CT concentration in each sample is interpolated from this standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ASPH CT elisa kit    ASPH CT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS748846. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ASPH CT. It may not necessarily be applicable to this product.
 NCBI GI #    258613955
 NCBI GeneID    444
 NCBI Accession #    NP_001158227.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001164755.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q6NXR7; Q8TB28; Q9H291; Q9H2C4; A6NDF4; A6NHI2; B4DIC9; B4E2K4; B7ZM95; E5RGP5; F5H667 [Other Products]
 UniProt Related Accession #    Q12797 [Other Products]
 Molecular Weight    28,926 Da
Table BarTOPTable Bar
 NCBI Official Full Name    aspartyl/asparaginyl beta-hydroxylase isoform k
 NCBI Official Synonym Full Names    aspartate beta-hydroxylase
 NCBI Official Symbol    ASPH [Similar Products]
 NCBI Official Synonym Symbols   
AAH; BAH; HAAH; JCTN; FDLAB; junctin; CASQ2BP1
[Similar Products]
 NCBI Protein Information    aspartyl/asparaginyl beta-hydroxylase; humbug; junctate; A beta H-J-J; cardiac junctin; ASP beta-hydroxylase; peptide-aspartate beta-dioxygenase; aspartyl/asparaginyl-beta-hydroxylase
 UniProt Protein Name    Aspartyl/asparaginyl beta-hydroxylase
 UniProt Synonym Protein Names   
Aspartate beta-hydroxylase; ASP beta-hydroxylase; Peptide-aspartate beta-dioxygenase
 UniProt Gene Name    ASPH [Similar Products]
 UniProt Synonym Gene Names    ASP beta-hydroxylase [Similar Products]
 UniProt Entry Name    ASPH_HUMAN
Section Bar
 NCBI Summary for ASPH CT    This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]
Section Bar
 UniProt Comments for ASPH CT    ASPH: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Belongs to the aspartyl/asparaginyl beta-hydroxylase family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.14.11.16

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum lumen; endoplasmic reticulum; integral to membrane; plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; electron carrier activity; structural constituent of muscle; calcium ion binding; structural molecule activity; peptide-aspartate beta-dioxygenase activity

Biological Process: positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; regulation of protein stability; palate development; pattern specification process; limb morphogenesis; response to ATP; peptidyl-aspartic acid hydroxylation; negative regulation of cell proliferation; activation of store-operated calcium channel activity; muscle contraction; regulation of inositol-1,4,5-triphosphate receptor activity; detection of calcium ion
Table BarTOPTable Bar
 Research Articles on ASPH CT    1. this study provides evidence that ASPH is mutated in a distinct form of syndromic ectopia lentis.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ASPH CT elisa kitDiseases associated with ASPH CT elisa kit
 Reference Product  PubMed Publications
 RYR2 elisa kit  >23 publications with ASPH CT and RYR2
 TRDN elisa kit  >13 publications with ASPH CT and TRDN
 CASQ2 elisa kit  >8 publications with ASPH CT and CASQ2
 INS elisa kit  >1 publications with ASPH CT and INS
 CASQ1 elisa kit  >1 publications with ASPH CT and CASQ1
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >23 publications with ASPH CT and Cardiovascular Diseases
 Heart Diseases Antibodies  >21 publications with ASPH CT and Heart Diseases
 Hypertrophy Antibodies  >10 publications with ASPH CT and Hypertrophy
 Disease Models, Animal Antibodies  >7 publications with ASPH CT and Disease Models, Animal
 Cardiomegaly Antibodies  >6 publications with ASPH CT and Cardiomegaly
 Nervous System Diseases Antibodies  >5 publications with ASPH CT and Nervous System Diseases
 Liver Neoplasms Antibodies  >4 publications with ASPH CT and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >4 publications with ASPH CT and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with ASPH CT and Liver Diseases
 Fibrosis Antibodies  >2 publications with ASPH CT and Fibrosis
Organs/Tissues associated with ASPH CT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >65 publications with ASPH CT and Muscle
 Heart Antibodies  >42 publications with ASPH CT and Heart
 Liver Antibodies  >8 publications with ASPH CT and Liver
 Kidney Antibodies  >5 publications with ASPH CT and Kidney
 Embryonic Tissue Antibodies  >4 publications with ASPH CT and Embryonic Tissue
 Lung Antibodies  >4 publications with ASPH CT and Lung
 Thymus Antibodies  >4 publications with ASPH CT and Thymus
 Brain Antibodies  >4 publications with ASPH CT and Brain
 Eye Antibodies  >3 publications with ASPH CT and Eye
 Spleen Antibodies  >3 publications with ASPH CT and Spleen
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions