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CNP elisa kit :: Human C Type Natriuretic Peptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721233
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

C Type Natriuretic Peptide (CNP), ELISA Kit

★Popular Item★
 Also Known As   

Human C Type Natriuretic Peptide ELISA Kit

 Product Gene Name   

CNP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600296
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CNP. No significant cross-reactivity or interference between CNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    250- 5000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CNP purchase    MBS721233 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C Type Natriuretic Peptide (CNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250- 5000pg/mL in biological research samples containing CNP, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS721233 kit is based on CNP antibody-CNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CNP elisa kit

   Intended Uses: This CNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CNP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CNP. Standards or samples are then added to the microtiter plate wells and CNP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CNP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CNP are added to each well to "sandwich" the CNP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CNP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.
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 Typical Standard Curve/Testing Data of CNP elisa kit    CNP elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS721233. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CNP. It may not necessarily be applicable to this product.
 NCBI GI #    13249346
 NCBI GeneID    4880
 NCBI Accession #    NP_077720.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024409.2 [Other Products]
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 UniProt Secondary Accession #    Q4ZG41 [Other Products]
 UniProt Related Accession #    P23582 [Other Products]
 Molecular Weight    13,246 Da
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 NCBI Official Full Name    C-type natriuretic peptide
 NCBI Official Synonym Full Names    natriuretic peptide C
 NCBI Official Symbol    NPPC [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    C-type natriuretic peptide; natriuretic peptide precursor C
 UniProt Protein Name    C-type natriuretic peptide
 Protein Family    2',3'-cyclic-nucleotide 3'-phosphodiesterase
 UniProt Gene Name    NPPC [Similar Products]
 UniProt Synonym Gene Names    CNP2 [Similar Products]
 UniProt Entry Name    ANFC_HUMAN
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 NCBI Summary for CNP    The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]
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 UniProt Comments for CNP    NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: extracellular space; secretory granule

Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding

Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; positive regulation of vasodilation; negative regulation of DNA metabolic process; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size
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 Research Articles on CNP    1. Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CNP elisa kitDiseases associated with CNP elisa kit
 Reference Product  PubMed Publications
 NPR2 elisa kit  >20 publications with CNP and NPR2
 NPPA elisa kit  >8 publications with CNP and NPPA
 NPR3 elisa kit  >7 publications with CNP and NPR3
 NPPB elisa kit  >6 publications with CNP and NPPB
 NPR1 elisa kit  >4 publications with CNP and NPR1
 Disease Name  Pubmed Publications
 Bone Diseases, Developmental Antibodies  >7 publications with CNP and Bone Diseases, Developmental
 Nervous System Diseases Antibodies  >5 publications with CNP and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >4 publications with CNP and Cardiovascular Diseases
 Growth Disorders Antibodies  >4 publications with CNP and Growth Disorders
 Hypertension Antibodies  >3 publications with CNP and Hypertension
 Disease Models, Animal Antibodies  >3 publications with CNP and Disease Models, Animal
 Heart Diseases Antibodies  >3 publications with CNP and Heart Diseases
 Cartilage Diseases Antibodies  >3 publications with CNP and Cartilage Diseases
 Hypertrophy Antibodies  >2 publications with CNP and Hypertrophy
 Demyelinating Diseases Antibodies  >1 publications with CNP and Demyelinating Diseases
Organs/Tissues associated with CNP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with CNP and Skin
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