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C3b elisa kit :: Human C3b ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2507442 C3b elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C3b, ELISA Kit

 Also Known As   

Human C3b (Complement Fragment 3b) ELISA Kit

 Product Gene Name   

C3b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    120700
 3D Structure    ModBase 3D Structure for P01024
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human C3b. No significant cross-reactivity or interference between Human C3b and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human C3b and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313--20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Other Notes    Small volumes of C3b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C3b purchase    MBS2507442 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C3b, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313--20ng/mL in biological research samples containing C3b, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2507442 kit is based on C3b antibody-C3b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3b. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for C3b elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C3b in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3b. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human C3b and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C3b. You can calculate the concentration of Human C3b in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2507442
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NCBI/Uniprot data below describe general gene information for C3b. It may not necessarily be applicable to this product.
 NCBI GI #    115298678
 NCBI GeneID    718
 NCBI Accession #    NP_000055.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000064.3 [Other Products]
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 UniProt Primary Accession #    P01024 [Other Products]
 UniProt Secondary Accession #    A7E236 [Other Products]
 UniProt Related Accession #    P01024 [Other Products]
 Molecular Weight    187,148 Da
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 NCBI Official Full Name    complement C3
 NCBI Official Synonym Full Names    complement component 3
 NCBI Official Symbol    C3 [Similar Products]
 NCBI Official Synonym Symbols   
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
[Similar Products]
 NCBI Protein Information    complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; acylation-stimulating protein cleavage product; complement component C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3
 UniProt Protein Name    Complement C3
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1Cleaved into the following 12 chains:Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg
 UniProt Gene Name    C3 [Similar Products]
 UniProt Synonym Gene Names    CPAMD1; C3bc; ASP [Similar Products]
 UniProt Entry Name    CO3_HUMAN
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 NCBI Summary for C3b    Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. A peptide (C3a) derived from the encoded protein has antimicrobial activity, so people with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Nov 2014]
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 UniProt Comments for C3b    C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway

Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
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 Research Articles on C3b    1. the 301T>C polymorphism of the ASP gene that influences the serum triglycerides level in the Uygur population, is associated with the development of coronary heart disease (CHD) and the CC genotype might be a risk factor of CHD
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C3b elisa kitPathways associated with C3b elisa kit
 Reference Product  PubMed Publications
 CR1 elisa kit  >431 publications with C3b and CR1
 CR2 elisa kit  >296 publications with C3b and CR2
 CFH elisa kit  >213 publications with C3b and CFH
 CD46 elisa kit  >198 publications with C3b and CD46
 CFI elisa kit  >75 publications with C3b and CFI
 ITGAM elisa kit  >73 publications with C3b and ITGAM
 ITGAX elisa kit  >19 publications with C3b and ITGAX
 CFD elisa kit  >4 publications with C3b and CFD
 C3AR1 elisa kit  >3 publications with C3b and C3AR1
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
Diseases associated with C3b elisa kitOrgans/Tissues associated with C3b elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >3683 publications with C3b and Kidney Diseases
 Cardiovascular Diseases Antibodies  >2422 publications with C3b and Cardiovascular Diseases
 Inflammation Antibodies  >1966 publications with C3b and Inflammation
 Proteinuria Antibodies  >1287 publications with C3b and Proteinuria
 Disease Models, Animal Antibodies  >1267 publications with C3b and Disease Models, Animal
 Lung Diseases Antibodies  >1017 publications with C3b and Lung Diseases
 Pain Antibodies  >1012 publications with C3b and Pain
 Brain Diseases Antibodies  >955 publications with C3b and Brain Diseases
 Necrosis Antibodies  >935 publications with C3b and Necrosis
 Liver Diseases Antibodies  >932 publications with C3b and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11930 publications with C3b and Blood
 Kidney Antibodies  >3533 publications with C3b and Kidney
 Bone Antibodies  >2985 publications with C3b and Bone
 Skin Antibodies  >2280 publications with C3b and Skin
 Brain Antibodies  >2136 publications with C3b and Brain
 Liver Antibodies  >1820 publications with C3b and Liver
 Muscle Antibodies  >1702 publications with C3b and Muscle
 Spinal Cord Antibodies  >1604 publications with C3b and Spinal Cord
 Nerve Antibodies  >1366 publications with C3b and Nerve
 Lung Antibodies  >1105 publications with C3b and Lung
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