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CAD elisa kit :: Human CAD ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2513383
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CAD, ELISA Kit

★Popular Item★
 Also Known As   

Human CAD (Caspase Activated Deoxyribonuclease) ELISA Kit

 Product Gene Name   

CAD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601883
 3D Structure    ModBase 3D Structure for O76075
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human CAD. No significant cross-reactivity or interference between Human CAD and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CAD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CAD purchase    MBS2513383 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CAD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing CAD, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2513383 kit is based on CAD antibody-CAD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CAD elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CAD concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CAD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CAD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CAD. You can calculate the concentration of CAD in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CAD elisa kit    CAD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2513383. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CAD. It may not necessarily be applicable to this product.
 NCBI GI #    3410909
 NCBI GeneID    1677
 NCBI Accession #    BAA32250.1 [Other Products]
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 UniProt Primary Accession #    O76075 [Other Products]
 UniProt Secondary Accession #    O60521; Q5SR22; Q9BYI4; Q9BYI5; Q9BYI6 [Other Products]
 UniProt Related Accession #    O76075 [Other Products]
 Molecular Weight    11,482 Da
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 NCBI Official Full Name    CAD
 NCBI Official Synonym Full Names    DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
 NCBI Official Symbol    DFFB [Similar Products]
 NCBI Official Synonym Symbols   
CAD; CPAN; DFF2; DFF40; DFF-40
[Similar Products]
 NCBI Protein Information    DNA fragmentation factor subunit beta; DNA fragmentation factor 40 kDa subunit; caspase-activated DNase; caspase-activated deoxyribonuclease; caspase-activated nuclease
 UniProt Protein Name    DNA fragmentation factor subunit beta
 UniProt Synonym Protein Names   
Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40
 Protein Family    Cadherin
 UniProt Gene Name    DFFB [Similar Products]
 UniProt Synonym Gene Names    CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40 [Similar Products]
 UniProt Entry Name    DFFB_HUMAN
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 NCBI Summary for CAD    Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
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 UniProt Comments for CAD    DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; Apoptosis; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; cytosol; nucleus

Molecular Function: enzyme binding; deoxyribonuclease activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis
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 Research Articles on CAD    1. DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CAD elisa kitPathways associated with CAD elisa kit
 Reference Product  PubMed Publications
 CASP3 elisa kit  >38 publications with CAD and CASP3
 DFFA elisa kit  >6 publications with CAD and DFFA
 DNASE2 elisa kit  >3 publications with CAD and DNASE2
 CIDEA elisa kit  >3 publications with CAD and CIDEA
 CIDEB elisa kit  >2 publications with CAD and CIDEB
 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
Diseases associated with CAD elisa kitOrgans/Tissues associated with CAD elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with CAD and Necrosis
 Carcinoma Antibodies  >7 publications with CAD and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with CAD and Adenocarcinoma
 Immune System Diseases Antibodies  >4 publications with CAD and Immune System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with CAD and Neoplasms, Experimental
 Cognition Disorders Antibodies  >3 publications with CAD and Cognition Disorders
 Cardiovascular Diseases Antibodies  >3 publications with CAD and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >2 publications with CAD and Disease Models, Animal
 Inflammation Antibodies  >2 publications with CAD and Inflammation
 Lung Neoplasms Antibodies  >1 publications with CAD and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with CAD and Brain
 Lung Antibodies  >3 publications with CAD and Lung
 Liver Antibodies  >3 publications with CAD and Liver
 Bone Antibodies  >2 publications with CAD and Bone
 Vascular Antibodies  >2 publications with CAD and Vascular
 Connective Tissue Antibodies  >2 publications with CAD and Connective Tissue
 Kidney Antibodies  >2 publications with CAD and Kidney
 Skin Antibodies  >1 publications with CAD and Skin
 Testis Antibodies  >1 publications with CAD and Testis
 Bone Marrow Antibodies  >1 publications with CAD and Bone Marrow
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