Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ANPEP rightarrow Human ANPEP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • SMAD3 Antibody
 • LSM2 Antibody
 • S100 Calcium Binding Protei n A9 (S100A9) ELISA Kit
 • Cystatin A (CSTA) Antibody
 • FSTL1 cDNA Clone
 • Paralemmin-1 (PALM) ELISA Kit
 • Annexin A2 (ANXA2) Recombinant Protein
 • FLT1 - VEGFR1 Antibody
 • GRF-1 Antibody
 • Acrylamide Biochemical
 • ABHD5 Antibody
 • Treponema pallidum p41 Recombinant Protein
 • alpha-SMA (ACTA2) Antibody
 • Apolipoprotein-J (CLU) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ANPEP elisa kit :: Human CD13/Aminopeptidase N ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177132
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD13/Aminopeptidase N (ANPEP), ELISA Kit

★Popular Item★
 Also Known As   

Human CD13/Aminopeptidase N PicoKine ELISA Kit

 Product Synonym Names    Aminopeptidase N; alanyl (membrane) aminopeptidase; Alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Alanyl membrane aminopeptidase; Aminopeptidase M; Aminopeptidase N; AMPN_HUMAN; ANPEP; AP M; AP N; AP-M; AP-N; APN; CD 13; CD13; CD13 antigen; gp150; hAPN; Lap 1; Lap1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN
 Product Gene Name   

ANPEP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS177132 COA    COA PDF
 OMIM    151530
 3D Structure    ModBase 3D Structure for P15144
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    Natural and recombinant Human CD13/Aminopeptidase N
Section Bar
 Assay Type    Quantitative Sandwich
 Detection Range    312pg/ml-20000pg/ml
 Sensitivity    <10pg/ml
Section Bar
 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANPEP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANPEP purchase    MBS177132 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD13/Aminopeptidase N (ANPEP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 312pg/ml-20000pg/ml in biological research samples containing ANPEP, with an estimated sensitivity of <10pg/ml. The ELISA analytical biochemical technique of the MBS177132 kit is based on ANPEP antibody-ANPEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANPEP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANPEP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ANPEP elisa kit

   Principle of the Assay: Human CD13/Aminopeptidase N ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD13/Aminopeptidase N has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: K69-K967) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13/Aminopeptidase N is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD13/Aminopeptidase N amount of sample captured in plate.

Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.
Section Bar
  Typical Testing Data/Standard Curve (for reference only) of ANPEP elisa kit    ANPEP elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS177132. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ANPEP. It may not necessarily be applicable to this product.
 NCBI GI #    143811362
 NCBI GeneID    290
 NCBI Accession #    P15144.4 [Other Products]
Section Bar
 UniProt Primary Accession #    P15144 [Other Products]
 UniProt Secondary Accession #    Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0 [Other Products]
 UniProt Related Accession #    P15144 [Other Products]
 Molecular Weight    109,540 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Aminopeptidase N
 NCBI Official Synonym Full Names    alanyl (membrane) aminopeptidase
 NCBI Official Symbol    ANPEP [Similar Products]
 NCBI Official Synonym Symbols   
APN; CD13; LAP1; P150; PEPN; GP150
[Similar Products]
 NCBI Protein Information    aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
 UniProt Protein Name    Aminopeptidase N
 UniProt Synonym Protein Names   
Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD_antigen: CD13
 Protein Family    Aminopeptidase
 UniProt Gene Name    ANPEP [Similar Products]
 UniProt Synonym Gene Names    APN; CD13; PEPN; AP-N; hAPN; AP-M [Similar Products]
 UniProt Entry Name    AMPN_HUMAN
Section Bar
 NCBI Summary for ANPEP    Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for ANPEP    CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Other Amino Acids Metabolism - glutathione; Protease; Receptor, misc.; EC 3.4.11.2; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q25-q26

Cellular Component: extracellular space; integral to plasma membrane; lysosomal membrane; ER-Golgi intermediate compartment; cytosol; external side of plasma membrane

Molecular Function: viral receptor activity; metallopeptidase activity; zinc ion binding; receptor activity; aminopeptidase activity

Biological Process: entry of virus into host cell; cellular protein metabolic process; angiogenesis; angiotensin maturation; proteolysis; cell differentiation
Table BarTOPTable Bar
 Research Articles on ANPEP    1. Molecular mechanisms regulating CD13-mediated adhesion.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ANPEP elisa kitPathways associated with ANPEP elisa kit
 Reference Product  PubMed Publications
 ENPEP elisa kit  >1 publications with ANPEP and ENPEP
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
Diseases associated with ANPEP elisa kitOrgans/Tissues associated with ANPEP elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >4 publications with ANPEP and Carcinoma
 Hypertension Antibodies  >3 publications with ANPEP and Hypertension
 Cardiovascular Diseases Antibodies  >2 publications with ANPEP and Cardiovascular Diseases
 Immune System Diseases Antibodies  >2 publications with ANPEP and Immune System Diseases
 Vascular Diseases Antibodies  >2 publications with ANPEP and Vascular Diseases
 Ovarian Neoplasms Antibodies  >1 publications with ANPEP and Ovarian Neoplasms
 Nervous System Diseases Antibodies  >1 publications with ANPEP and Nervous System Diseases
 Fibrosis Antibodies  >1 publications with ANPEP and Fibrosis
 Breast Neoplasms Antibodies  >1 publications with ANPEP and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with ANPEP and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with ANPEP and Blood
 Prostate Antibodies  >3 publications with ANPEP and Prostate
 Embryonic Tissue Antibodies  >3 publications with ANPEP and Embryonic Tissue
 Bone Antibodies  >3 publications with ANPEP and Bone
 Kidney Antibodies  >2 publications with ANPEP and Kidney
 Uterus Antibodies  >2 publications with ANPEP and Uterus
 Connective Tissue Antibodies  >2 publications with ANPEP and Connective Tissue
 Pancreas Antibodies  >2 publications with ANPEP and Pancreas
 Bone Marrow Antibodies  >2 publications with ANPEP and Bone Marrow
 Vascular Antibodies  >2 publications with ANPEP and Vascular
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions