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CDK-9 elisa kit :: Human CDK9 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502653
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDK9 (CDK-9), ELISA Kit

★Popular Item★
 Also Known As   

Human CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

 Product Gene Name   

CDK-9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2502653 COA    COA PDF
 OMIM    603251
 3D Structure    ModBase 3D Structure for P50750
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and some recombinant Human CDK9. No significant crossreactivity or interference between Human CDK9 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDK-9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDK-9 purchase    MBS2502653 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CDK9 (CDK-9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing CDK-9, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2502653 kit is based on CDK-9 antibody-CDK-9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK-9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK-9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDK-9 elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CDK9 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CDK9. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CDK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CDK9, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK9. The concentration of Human CDK9 in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CDK-9 elisa kit    CDK-9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502653. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDK-9. It may not necessarily be applicable to this product.
 NCBI GI #    4502747
 NCBI GeneID    1025
 NCBI Accession #    NP_001252.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001261.3 [Other Products]
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 UniProt Primary Accession #    P50750 [Other Products]
 UniProt Secondary Accession #    Q5JU24; Q5JU25; Q5U006; Q96TF1 [Other Products]
 UniProt Related Accession #    P50750 [Other Products]
 Molecular Weight    53,365 Da
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 NCBI Official Full Name    cyclin-dependent kinase 9
 NCBI Official Synonym Full Names    cyclin-dependent kinase 9
 NCBI Official Symbol    CDK9 [Similar Products]
 NCBI Official Synonym Symbols   
TAK; C-2k; CTK1; CDC2L4; PITALRE
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase 9; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit
 UniProt Protein Name    Cyclin-dependent kinase 9
 UniProt Synonym Protein Names   
C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
 Protein Family    Probable cyclin-dependent kinase
 UniProt Gene Name    CDK9 [Similar Products]
 UniProt Synonym Gene Names    CDC2L4; TAK [Similar Products]
 UniProt Entry Name    CDK9_HUMAN
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 NCBI Summary for CDK-9    The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CDK-9    CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Cell cycle regulation; EC 2.7.11.23; Nuclear receptor co-regulator; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; CMGC group; CDK family; CDK/CDK9 subfamily; CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; positive regulation of histone phosphorylation; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest
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 Research Articles on CDK-9    1. Plk1 negatively regulates the RNA polymerase II-dependent transcription through inhibiting the activity of cyclin T1/Cdk9 complex.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDK-9 elisa kitPathways associated with CDK-9 elisa kit
 Reference Product  PubMed Publications
 CCNT1 elisa kit  >128 publications with CDK-9 and CCNT1
 HEXIM1 elisa kit  >51 publications with CDK-9 and HEXIM1
 BRD4 elisa kit  >28 publications with CDK-9 and BRD4
 CCNT2 elisa kit  >15 publications with CDK-9 and CCNT2
 CCNK elisa kit  >11 publications with CDK-9 and CCNK
 AFF4 elisa kit  >10 publications with CDK-9 and AFF4
 SUPT5H elisa kit  >8 publications with CDK-9 and SUPT5H
 LARP7 elisa kit  >2 publications with CDK-9 and LARP7
 MLLT1 elisa kit  >1 publications with CDK-9 and MLLT1
 POLR2A elisa kit  >1 publications with CDK-9 and POLR2A
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 HIV Transcription Elongation Pathway antibodies  HIV Transcription Elongation Pathway Diagram
 HIV Elongation Arrest And Recovery Pathway antibodies  HIV Elongation Arrest And Recovery Pathway Diagram
Diseases associated with CDK-9 elisa kitOrgans/Tissues associated with CDK-9 elisa kit
 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >29 publications with CDK-9 and Hypertrophy
 Inflammation Antibodies  >17 publications with CDK-9 and Inflammation
 Necrosis Antibodies  >17 publications with CDK-9 and Necrosis
 Heart Diseases Antibodies  >14 publications with CDK-9 and Heart Diseases
 Breast Neoplasms Antibodies  >10 publications with CDK-9 and Breast Neoplasms
 Lung Diseases Antibodies  >8 publications with CDK-9 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >8 publications with CDK-9 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >7 publications with CDK-9 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with CDK-9 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >3 publications with CDK-9 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >35 publications with CDK-9 and Muscle
 Heart Antibodies  >17 publications with CDK-9 and Heart
 Lung Antibodies  >11 publications with CDK-9 and Lung
 Bone Antibodies  >11 publications with CDK-9 and Bone
 Embryonic Tissue Antibodies  >10 publications with CDK-9 and Embryonic Tissue
 Brain Antibodies  >10 publications with CDK-9 and Brain
 Liver Antibodies  >9 publications with CDK-9 and Liver
 Kidney Antibodies  >8 publications with CDK-9 and Kidney
 Prostate Antibodies  >6 publications with CDK-9 and Prostate
 Bone Marrow Antibodies  >5 publications with CDK-9 and Bone Marrow
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